Altered expression of SPL transcription factors in ago5 mutants. (A) RT-qPCR analysis of SPL3/4/5 gene expression in the SAM of 20-day-old Col-0, ago5-1, ago5-5 plants. (B) RT-qPCR analysis of SPL9/11/15 gene expression in the SAM of 20-day-old Col-0, ago5-1, ago5-5 plants. Values are mean ± SD of three biological replicates and are given as fold change compared to Col-0. Asterisks indicate significant differences from Col-0 in a Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Error bars indicate standard deviation.