Increased PAX6 mediated TRPC6 upregulation in DRG neurons following chemotherapeutics treatment. (A) The double immunofluorescence staining indicated colocalization of PAX6 with TRPC6 in DRG neurons of rats (n = 4 in each group), scale bar = 100 μm. (B–D) Intrathecal injection of PAX6 siRNA significantly prevented the chemotherapeutics-induced TRPC6 upregulation (n = 4 in each group, **P < .01 vs the vehicle group, ##P < .01 vs the corresponding chemotherapeutics group). (E–G) Following PAX6 siRNA application (i.t.), ChIP assay was performed with PAX6 antibody on day 15 after chemotherapeutics treatment in rats (n = 3 in each group, **P < .01 vs the vehicle group, ##P < .01 vs the corresponding chemotherapeutic group). BTZ, Bortezomib; Oxal, Oxaliplatin; Pacl, Paclitaxel; Veh, Vehicle.