The change of PAX6 gene methylation level followed chemotherapeutics treatment. (A) RRBS results showed the change of methylation level in TRPC6 loci before and after oxliplatin treatment. (B) Circos diagram showed DMRs distributed globally across all chromosomes. (C) The Gene Ontology (GO) pathway analysis showed the enrichment degree of biological process with differential DMR mapped genes. (D) Venn diagram showed the overlap of genes between GO analysis and Genomatix database. (E-G) MeDIP assay was performed to detect the change of DNA methylation level in specific regions of PAX6 and IRX5 loci after chemotherapeutics treatment for 15 days in DRG (n = 3 in each group; *P < .05, **P < .01 vs the correspondence vehicle group). BTZ, Bortezomib; IR, IRX5; Oxal, Oxaliplatin; Pacl, Paclitaxel; PA, PAX6; TFs, Transcription factors; Veh, Vehicle.