DEA-C3D potassium salt causes a reduction in P. aeruginosa clinical CF isolate PA68 biofilms alone and in combination with tobramycin. (a) Total biofilm biomass, representing live and dead biomass combined. (b) Biofilm thickness distribution. (c) Total biofilm surface area coverage. Values for all parameters were obtained from COMSTAT analysis of CLSM images. Scatter plots show all data points from n=4, with the mean and SD. One-way ANOVA with Tukey’s multiple comparisons tests were used for statistical analyses. TOB, tobramycin.