Southern blot hybridization of worf (A) and spock (B), using D. miranda strain MSH22. (A) Hybridization to the worf probe (lanes A–H). Lane A: D. miranda female DNA, HinfI; lane B: D. miranda male DNA, HinfI; lane C: D. pseudoobscura female DNA, HinfI; lane D: D. pseudoobscura male DNA, HinfI; lane E: D. miranda female DNA, PstI; lane F: D. miranda male DNA, PstI; lane G: D. pseudoobscura female DNA, PstI; lane H: D. pseudoobscura male DNA, PstI. (B) Hybridization to the spock probe (lanes I–P). Lane I: D. miranda female DNA, MscI; lane J: D. miranda male DNA, MscI; lane K: D. pseudoobscura female DNA, MscI; lane L: D. pseudoobscura male DNA, MscI; lane M: D. miranda female DNA, EcoRV; lane N: D. miranda male DNA, EcoRV; lane O: D. pseudoobscura female DNA, EcoRV; lane P: D. pseudoobscura male DNA, EcoRV. (C) Hybridization with the pic gene from D. melanogaster (as an external control for monitoring equal amounts of genomic DNA, lanes Q–T). Lane Q: D. miranda female DNA, EcoRI; lane R: D. miranda male DNA, EcoRI; lane S: D. pseudoobscura female DNA, EcoRI; lane T: D. pseudoobscura male DNA, EcoRI