In situ hybridization of D. miranda for worf and spock. Arrows point to hybridization signals. The star indicates the neo-X chromosome. (A) Female nucleus of D. miranda (strain 0101.4). Hybridization of worf; 24 insertions were detected. (B) Neo-Y chromosome hybridized with worf; about 30 insertions were observed. (C) D. miranda female nucleus (strain MSH38). Hybridization of spock; 16 insertions were detected. (D) Neo-Y chromosome hybridized with spock; about 25 insertions were observed