H19 overexpression inhibits in vivo growth of the contralateral GH3 tumor. (A) Normal GH3 cells were injected into the left flank and lentivirus-transfected GH3 cells were injected into the right flank of nude mice in group A, whereas normal GH3 cells were injected into the left flank and H19-overexpressing GH3 cells were injected into the right flank of nude mice in group B, both at the concentration of 1 × 106 cells (n = 8); the mice were euthanized after a 2-wk culture. (B) The tumors were removed, photographed and weighed. (C) The mean weight of the tumors on the contralateral H19 side was significantly lower than that on the normal GH3 side. ***P < 0.0001. (D and E) The volume and growth rate of the subcutaneous tumor formation were measured daily using the following equation: volume = a × b2/2, where a represents the length and b represents the width. ***P < 0.0001. (F) The H19 expression level in the four groups was detected and H19 RNA was measured by qRT-PCR and normalized to β-actin.
