Cross-talk between G protein–coupled receptors AT1 and GPER-1. (A) Aldosterone in addition to Ang II increased the expression of CYP11B2 gene in APA strips. (B) CYP11B2 gene expression in APA strips after pretreatment with aldosterone and Ang II in addition to irbesartan, G36, or both. (C) Aldosterone in addition to Ang II increased the expression of CYP11B2 gene in HAC15 cells. (D) CYP11B2 gene expression in HAC15 cells after pretreatment with aldosterone and Ang II on top of irbesartan or G36, or both. For (A) to (D) data represent mean ± SD of four different experiments performed in duplicate. (E) Representative results of coimmunoprecipitation experiments performed in HAC15 cells; after AT1R immunoprecipitation, GPER protein expression was detected by immunoblot; the presence of heterodimers in GPER-immunoprecipitated protein was also confirmed (n = 3). Rabbit IgG control: negative control. ALDO, aldosterone. ^#P = 0.04; *P = 0.03; **P < 0.01; ***P < 0.001.