Functional characterization of the TBL1X p.Asn382del mutation. Western blotting, performed with use of N-1 cells, showed similar protein expression levels between WT and (A) the mutant. Cyclophilin was used as a loading control. Note that N-1 cells express endogenous mouse Tbl1x protein with a relatively low level. (B) In the luciferase assay using the Trh-luc reporter, LIA of Trh-luc was observed with coexpression of TRβ1 and NCoR. The LIA was further augmented with addition of WT-TBL1X, but was abrogated with addition of Asn382-del-TBL1X. (C) Similar results were obtained with the TSHB-luc reporter. The data of the luciferase assays are representative of three experiments showing similar results. Bars indicate SEM.