Metabolic activity of leiomyoma cells after PLIN2 knockdown. Leiomyoma cells were transfected with control siRNA or PLIN2 siRNA for 72 h. Metabolic activity was determined by the measurement of extracellular flux using the Seahorse XF 96 Analyzer. The OCR reflects mitochondrial respiration, whereas the ECAR reflects glycolysis. Basal activity represents metabolic activity under basal conditions. Stress response represents metabolic activity after injection of FCCP and oligomycin. (A and B) Representative kinetics of increased (A) OCR and (B) ECAR over time in control or PLIN2-depleted cells. (C and D) Quantification of data from five patient samples demonstrates that PLIN2 depletion is associated with increased (C) mitochondrial respiration (mean ± SEM, ***P < 0.0001) and (D) glycolysis (mean ± SEM, ***P < 0.0001) under both basal and stressed conditions. (E and F) Percent change in OCR and ECAR transitioning from the basal state to the stress state. PLIN2 depletion causes leiomyoma cells to rely more heavily on (F) glycolysis (73 ± 21.3% vs 64 ± 21.3%, n = 5, ***P < 0.0001) over (E) mitochondrial respiration (115% vs 117%, n = 5, P = 0.63) to meet increased energy demand.