Abundance of inflammatory cells in bronchoalveolar lavage fluid (BALF) from wildtype (WT) and Cyp2abfgs-null mice after acute environmental tobacco smoke (ETS) inhalation. WT and Cyp2abfgs-null mice (adult female) were exposed daily (40 min per day) to filtered air (FA) or ETS for 1 or 2 weeks as described in Materials and Methods section. Bronchoalveolar lavage was performed 24 h after completion of the last exposure. A, Total cells were counted manually using a hemocytometer. Total numbers of macrophages (B), neutrophils (C), and lymphocytes (D) were determined using cytopreparations. Data represent means ± SD (n = 3). *p < .05, **p < .01, ***p < .001, ****p < .0001 (two-way ANOVA, followed by Tukey’s multiple comparisons test).