Everolimus treatment influences 24-h urine steroid metabolome. Steroid hormone metabolites were normalized to 24-h creatinine to account for possible incomplete urine collection. The 24-h excretion of (A) aldosterone (P = 0.629), (B) TH-Aldo (P = 0.732), (C) F (P = 0.100), (D) total glucocorticoid metabolites (THF, α-THF, THE, α- and β-cortolone, β-cortol, cortexolone, F, and E; P = 0.976), (E) testosterone (P = 0.470), (F) 11β-hydroxyandrosterone (P = 0.470), and (G) progesterone (P = 0.117) was not significantly different after a 2-wk everolimus treatment (V5, d15) and a 2-wk washout (V6, d28) compared with baseline (V3, d0). Subjects who showed a marked decrease of plasma aldosterone after everolimus treatment are marked as responders in red; nonresponders are marked in gray. Data are shown with median and IQR. Data analysis with nonparametric one-way ANOVA (Friedman) and Dunnett post hoc test. n = 11 as baseline 24-h urine for steroid metabolome from subject one was not collected.