Changes of action potential in hiPSC-CMs from the BrS patient. APs were evoked by pulses of 1 nA for 3 ms at 1 Hz in current clamp mode. When APs reached steady state, 10 sequential APs were recorded and mean values were calculated for each cell. The AP parameters were analysed by computer using an ISO-3 multitasking patch-clamp programme (MFK M. Friedrich, Niedernhausen, Germany). (A) Representative traces of APs in donor (D1, D2, and D3) and BrS cells. (B) Mean values of APA. (C) Mean values of maximal depolarization velocity (V_max) of APs. (D) Mean values of RPs. (E) Mean values of AP duration at 50% repolarization (APD50). (F) Mean values of AP duration at 90% repolarization (APD90). AP, action potential; APA, action potential amplitude; BrS, Brugada syndrome; hiPSC-CMs, human-induced pluripotent stem cell-derived cardiomyocytes; n, number of cell; n.s., not statistically significant; RP, resting potential.