(A) The generated iPSC lines isBrSd1.9, isBrSd1.12, and isBrSd1.23 generated from skin fibroblasts of the BrS patient display a typical morphology for human pluripotent stem cells (upper panel) and are positive for alkaline phosphatase (lower panel). (B) In comparison to donor’s fibroblasts, generated iPSC lines show expression of endogenous pluripotency markers OCT4, SOX2, NANOG, LIN28, FOXD3, and GDF3 at mRNA level proven by RT-PCR. hESCs were used as positive control, MEFs were used as negative control. (C) Generated iPSC lines express pluripotency markers OCT4, SOX2, NANOG, LIN28, SSEA4, and TRA-1-60 as shown by immunofluorescence staining. Nuclei are co-stained with DAPI. Scale bar: 100 µm. (D) Flow cytometry analysis of pluripotency markers OCT4 and TRA-1-60 reveals a homogeneous population of pluripotent cells in generated iPSC lines. (E) Spontaneous differentiation potential of generated iPSC lines was analysed by embryoid body (EB) formation. Immunocytochemical staining of spontaneously differentiated iPSC lines shows expression of endodermal marker AFP, mesodermal-specific α-SMA, and ectodermal βIII-tubulin. Nuclei are co-stained with DAPI. Scale bar: 100 µm. BrS, Brugada syndrome; hESCs, human embryonic stem cells; iPSC, induced pluripotent stem cell; MEFs, mouse embryonic fibroblasts.