Translocation of AHR to the nucleus and target gene expression upon exposure to different classes of ligands. Upon 16 h of treatment with TCDD (10 nM), BNF (5 μM), SGA360 (10 μM), or SGA315 (10 μM), (A) protein levels of AHR were examined in subcellular fractions by immunoblot analyses, or (B) CYP1A1 expression was assessed by qRT-PCR. Data are presented as mean ± SD, n = 3; significance was determined by one-way ANOVA.