Effect of kinase inhibitors and their combination with vorinostat on cell dispersion. (A and B) Experimental results of A549 dispersion in spheroids before and after treatment with 1 µM dasatinib as a SRC kinase inhibitor for 84 h cocultured with HUVECs, respectively. (C). Measurement of dispersion distance of A549 cells from collagen spheroids in dasatinib and no treatment control normalized by dividing to distance at 0 time-point. (D and E) Treatment of A549 spheroids and monitoring cell dispersion with 1 µM aspirin or sulfasalazine for 84 h as IKBK inhibitors. (F). Normalized dispersion distance following treatment with aspirin or sulfasalazine for 84 h. (G and H) Significant (t-test, P-value < 0.05) inhibition of A549 dispersion distance normalized to 0 h time-point from the spheroids following co-treatment with dasatinib and sulfasalazine at 84 h. (I and J) Assessment of cell dispersion following combination treatment with 1 µM of each drug for 84 h. Significant (t-test, P-value < 0.05) dispersion distance between null control versus combinatorial triple treatment at 84 h was observed. Data represent mean± SEM, and the error bars indicate dispersion measurements in two independent spheroids. Scale bar indicates 50 µm. (K) Distance between dissociated A549 and HUVEC cells normalized to 0 h as a measure of endothelial cell migration toward carcinoma cells.