MtEPP1 expression is induced in the root hairs in response to NF. Transgenic roots from Jemalong A17 (A–D) and the nfp-1 (E–H) mutant plant expressing the pMtEPP1::GUS construct were treated for 1 h with water (mock) or 10 nM NF. (A, B) and (E, F) The pMtEPP1::GUS activity in whole roots from Jemalong A17 and nfp-1 mutant plants, respectively. (C–D) and (G–H) The pMtEPP1::GUS activity in root hairs of roots from Jemalong A17 and nfp-1 mutant plants, respectively. Both mock- and NF-treated transgenic roots were stained for 1 h at 37°C. Scale bars represent 500 μm for (A), (B), (E) and (F), and 200 μm for (C), (D), (G) and (H). Images shown are representative of five biological replicates, each one containing 10 transgenic roots.