Surface plasmon resonance dissociation curves for the interaction of clotrimazole and the PxrAB/Tü variant. The interaction analysis between the MBP-Pxr LBD and ligand was performed with both multi and single cycle kinetics at the same conditions (25°C). Surface plasmon resonance sensorgram for the receptor-ligand interaction was fitted globally using a 1:1 Langmuir interaction model. Surface plasmon resonance dissociation curves for the interaction between CLO and PxrTL, PxrSWT, and PxrUNK are shown in Supplementary Figure 3.