—(a) Alignment of the predicted accD protein sequences from the Droseraceae species compared with that of Fagopyrum esculentum, shaded to indicate conservation. Black indicates amino acids identical to those in the F. esculentum sequence. Sequences are numbered as follows: 1, F. esculentum; 2, Aldrovanda vesiculosa; 3, Dionaea muscipula; 4, Drosera regia; 5, Drosera erythrorhiza; and 6, Drosera rotundifolia. The sequences from D. muscipula, Dr. erythrorhiza, and Dr. rotundifolia are truncated at the N-terminus, contain large indels, and in the case of D. muscipula, a long C-terminal extension due to mutation of the usual stop codon. The functionality of these sequences is doubtful. (b) Alignment of the trnG-UCC gene from the Droseraceae species compared with that of F. esculentum, shaded to indicate conservation. Black indicates nucleotides identical to those in the F. esculentum sequence. The positions of the tRNA exons are indicated by magenta arrows. Sequences are numbered as in (a). Alignment and visualization was done with Geneious 9.1.5 (http://www.geneious.com, Kearse et al. 2012). (c) Cloverleaf representation of the trnG-UCC sequence from F. esculentum, indicating nucleotide changes in the Droseraceae (magenta for D. muscipula, orange for Dr. rotundifolia, and red for Dr. sera erythrorhiza). Many of these nucleotide changes would be expected to severely impact the function of the tRNA, most obviously the alteration in the anticodon in Dr. erythrorhiza.