Optogenetic inhibition of the mPFC during trace interval also impairs the TEC acquisition. (E) Scheme for optical fiber implant site and 590-nm LED illumination pattern to bilateral caudal mPFC during trace interval period. (B,C) Effects of optogenetic inhibition of the caudal mPFC during the trace interval period on CR% and CR peak amplitude of TEC acquisition (*P < 0.05, **P < 0.05,***P < 0.001; 2-way ANOVA with repeated measures followed by Tukey post hoc test). Data are represented as mean ± s.e.m.