Sulfation and sialylation changes in uterine glycocalyx after the GP3ST+FucT-III transfection. Alcian blue (A–D) and Maackia amurensis mitogen (MAM) lectin (E–G) staining in uterine tissues 24 h after gene transfection of GP3ST+FucT-III (βGal 3-O-sulfotransferase (GP3ST)-alpha (1,3/1,4)fucosyltransferase) (B-D, F-G) or LacZ (β-galactosidase, as a control group; A, E). Methylation (G) or combined methylation-saponification (H) pretreatment before alcian blue staining, or neuraminidase pretreatment before MAM lectin staining (I) in uterine tissues from the GP3ST+FucT-III group. Lu, lumen; Gl, gland. Scale bars: 100 μm.