Heterochromatin state of the chromosomal region chrX:66,763,273-66,763,602. The difference between digested and undigested DNA was calculated on the cycle thresholds (Ct) of the quantitative PCR run and reflects the level of nuclease accessibility. GF-183 and GF-32 are control GFs, GF-72 and GF-70 are AR mutation–positive GFs from patients with CAIS, and GF-89 is an AIS type II–derived GF. All experiments were performed in triplicate. Error bars indicate the SD.