A proof of principle 3C-seq/r3Cseq analysis on the well-characterized β-globin locus. Gene locations are shown at the top followed below by a map of restriction fragments. The line plots show overall detected cis-interaction signals (40 kb up- and 60 kb downstream of the viewpoint) with the β-major promoter in both FL and FB cells. High signals on the viewpoint fragment or the immediately adjacent fragments were excluded. Color gradients represent the range of significant interaction signals (q-value). The bar plot represents the ratio (log2) of normalized signal between FL and FB. The light blue box highlights the LCR region with its hypersensitive sites (HS1-5), coinciding with several FL-specific significant interaction regions and binding sites of transcription factor complexes.