(A) Real-time monitoring of the fluorescence of stGFP synthesized in the reconstituted T. thermophilus system at 37°C (grey solid line: with stGFP mRNA and grey dashed line: without mRNA) and 50°C (black solid line: with stGFP mRNA and black dashed line: without mRNA). Inset: western blot analysis of aliquots taken from the 50°C reaction containing stGFP mRNA at different time points using an antibody specific to stGFP. (B) SDS–PAGE analysis of aliquots of the translation reactions at 4 h in the presence (lane 6) or absence (lane 5) of stGFP mRNA. Different amounts of purified recombinant stGFP are loaded on the same gel in lane 1 (200 μg/ml), lane 2 (100 μg/ml) and lane 3 (50 μg/ml). The MW standards (run in lane 4) are indicated on the left.