Figure 6.
![(A) The NER dual incision activity of cancer cells extracts. Model DNAs were incubated for 30 min at 30°C with cell extracts prepared from HeLa, C33A or SiHa cells (20 nМ DNA, 1.6 mg/ml of extract proteins and 500 nM template 1). The excision products were detected by annealing to template followed by end-labelling using α-[32P]-dCTP and Taq DNA polymerase. The reaction products were resolved on a 10% denaturing polyacrylamide gel. Non-modified 137-bp DNA was used as a negative control; 32P-labelled oligonucleotides were used as size marker. (B) Densitometric analysis of the lanes 5 (HeLa, left), 8 (C33A, middle) and 11 (SiHa, right).](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/41/12/10.1093_nar_gkt301/4/gkt301f6p.jpeg?Expires=1750372438&Signature=NoopoxYOnhhAZJiflIVPxTk1D4yP3wDcgF-EHHGpINyGHf5S5fzQZH8DF4pPiPKF6psoqyUJ3tTlNlh-5RAIKKEfkSFTQ05X3XxN5nKBRtt8-IlULpPjXY9pv~MmPD8BOm9q7ZCmEkQ0SElADYthOjaXneY-m30G-a2mt6QqJkNv9Ny-CUyLgab4pg1JnjI5IztFpsFQiZlQIQkrnJWk~-zxmet3iM1rDZV0tKtzXdDvI2HjKo-PrWew~LuYbyQtnWDH590QYjkOK0U7n7pS5BGJoMDNht15TUZ5~4A4P7w0X5X~NqQT6PkAmCJ51mVCKt-U-AyPrKXvZ5356ep-IA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
(A) The NER dual incision activity of cancer cells extracts. Model DNAs were incubated for 30 min at 30°C with cell extracts prepared from HeLa, C33A or SiHa cells (20 nМ DNA, 1.6 mg/ml of extract proteins and 500 nM template 1). The excision products were detected by annealing to template followed by end-labelling using α-[32P]-dCTP and Taq DNA polymerase. The reaction products were resolved on a 10% denaturing polyacrylamide gel. Non-modified 137-bp DNA was used as a negative control; 32P-labelled oligonucleotides were used as size marker. (B) Densitometric analysis of the lanes 5 (HeLa, left), 8 (C33A, middle) and 11 (SiHa, right).
