Single-nucleotide polymorphisms (SNPs) and indels in the Crimean-Congo hemorrhagic fever virus (CCHFV) population during and after ribavirin treatment. A, SNP frequencies (left y-axis) across each segment (x-axis) display intrahost viral diversity. Each data point is colored according to each sample in which the SNP frequency was ≥2% at each position. The boxes above the graphs represent open-reading frames corresponding to the nucleoprotein (NP), mucin, GP38 (38), Gn, Nsm (N), Gc, and RdRP domains. B, Rates of transitions (trs), transversions (trv), and indels per site per genome (left y-axis) were measured for each sample (x-axis). The indels were significantly accumulated in the L segment (P = .0202, by 2-way analysis of variance). Viral load was mapped on the right y-axis as a reference. Closed circles indicate samples collected during ribavirin treatment. Open circles indicate samples collected after the cessation of ribavirin treatment. C, The frequency of base changes in samples, by day after treatment initiation. The total number of positions analyzed involve 854 nucleotides distributed across the L segment. Values are the sum of base changes per site per genome for positions where the SNP frequency was ≥2%.