Fig. 4
Stage-dependent accumulation of arabinogalactan proteins in hyphal cell walls. Cuscuta campestris haustorium tips at 48 hours after attachment (haa) (A–D) and 72 haa (E–H) stained with β-glucosyl Yariv (βGlcY) (A and E), α-galactosyl Yariv (αGalY) (B and F), LM6 antibody (C and G) or LM2 antibody (D and H). Cc, C. campestris; At, Arabidopsis thaliana; pha, pre haustorium; ha, haustorium. Asterisks indicate searching hyphae. Arrows indicate cells in the holdfast. Scale bar = 50 µm. LM6 and LM2 antibodies were detected using Alexa Flour® 488-conjugated secondary antibody (green), and the section was counterstained with calcofluor white (blue).

Stage-dependent accumulation of arabinogalactan proteins in hyphal cell walls. Cuscuta campestris haustorium tips at 48 hours after attachment (haa) (A–D) and 72 haa (E–H) stained with β-glucosyl Yariv (βGlcY) (A and E), α-galactosyl Yariv (αGalY) (B and F), LM6 antibody (C and G) or LM2 antibody (D and H). Cc, C. campestris; At, Arabidopsis thaliana; pha, pre haustorium; ha, haustorium. Asterisks indicate searching hyphae. Arrows indicate cells in the holdfast. Scale bar = 50 µm. LM6 and LM2 antibodies were detected using Alexa Flour® 488-conjugated secondary antibody (green), and the section was counterstained with calcofluor white (blue).

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