Mean ± SEM Values for Animal Morphometry, Fetal Plasma Cortisol, and Lung Measurements in the 3 Treatment Groups
| . | Saline (n = 13) . | 0.5 LEP (n = 10) . | 1.0 LEP (n = 7) . |
|---|---|---|---|
| Maternal body weight (kg) | 44.8 ± 2.2 | 44.1 ± 2.4 | 45.6 ± 3.8 |
| Total placentome weight (g) | 319 ± 22 | 298 ± 18 | 267 ± 35 |
| Fetal body weight (kg) | 2.76 ± 0.16 | 2.74 ± 0.12 | 2.32 ± 0.19 |
| CRL (cm) | 43.0 ± 1.0 | 43.5 ± 0.7 | 41.4 ± 1.1 |
| BMI (kg/m2) | 14.8 ± 0.4 | 14.4 ± 0.5 | 13.3 ± 0.4 |
| PI (kg/m3) | 34.4 ± 0.9 | 33.2 ± 1.2 | 32.2 ± 0.5 |
| Plasma cortisol concentration (ng/mL) | |||
| Basal | 8.4 ± 1.2 | 6.6 ± 0.8 | 6.1 ± 1.4 |
| Day 5 | 10.4 ± 2.1 | 7.1 ± 1.3 | 12.0 ± 3.7 |
| Absolute lung weight (g) | 74.4 ± 4.5 | 74.1 ± 5.2 | 64.0 ± 4.3 |
| Relative lung weight (% of body weight) | 2.71 ± 0.10 | 2.71 ± 0.16 | 2.80 ± 0.13 |
| Pulmonary water content (%) | 89.1 ± 0.2 | 89.1 ± 0.4 | 89.3 ± 0.4 |
| Pulmonary glycogen content (mg/g) | 2.50 ± 0.28 (n = 6) | 2.15 ± 0.16 | 2.54 ± 0.37 |
| Alveolar air-space (%) | 49.7 ± 2.8 | 54.8 ± 0.7 | 53.0 ± 3.5 |
| Alveolar tissue (%) | 44.4 ± 2.8 | 39.6 ± 0.8 | 38.7 ± 2.4 |
| Extra-alveolar air-space (%) | 2.7 ± 0.3 | 2.4 ± 0.2 | 3.0 ± 0.4 |
| Extra-alveolar tissue (%) | 3.2 ± 0.3 | 3.2 ± 0.3 | 5.4 ± 1.1 |
| Alveolar wall thickness (harmonic mean, μm) | 9.28 ± 0.66a | 7.15 ± 0.25b | 9.88 ± 0.43a |
| Alveolar surface area (cm2/cm3) | 707 ± 139 | 535 ± 143 | 911 ± 388 |
| Secondary septal crest density (number/mm2) | 404 ± 33 | 548 ± 65* | 527 ± 69 |
| Relative volume of elastin | |||
| Interalveolar septae | 28.9 ± 1.5a | 35.1 ± 1.8b | 31.4 ± 2.0a,b |
| Secondary septal crests | 11.2 ± 1.1 | 12.7 ± 1.4 | 11.6 ± 1.1 |
| Total | 40.2 ± 1.8a | 47.8 ± 2.6b | 43.0 ± 2.4a,b |
| . | Saline (n = 13) . | 0.5 LEP (n = 10) . | 1.0 LEP (n = 7) . |
|---|---|---|---|
| Maternal body weight (kg) | 44.8 ± 2.2 | 44.1 ± 2.4 | 45.6 ± 3.8 |
| Total placentome weight (g) | 319 ± 22 | 298 ± 18 | 267 ± 35 |
| Fetal body weight (kg) | 2.76 ± 0.16 | 2.74 ± 0.12 | 2.32 ± 0.19 |
| CRL (cm) | 43.0 ± 1.0 | 43.5 ± 0.7 | 41.4 ± 1.1 |
| BMI (kg/m2) | 14.8 ± 0.4 | 14.4 ± 0.5 | 13.3 ± 0.4 |
| PI (kg/m3) | 34.4 ± 0.9 | 33.2 ± 1.2 | 32.2 ± 0.5 |
| Plasma cortisol concentration (ng/mL) | |||
| Basal | 8.4 ± 1.2 | 6.6 ± 0.8 | 6.1 ± 1.4 |
| Day 5 | 10.4 ± 2.1 | 7.1 ± 1.3 | 12.0 ± 3.7 |
| Absolute lung weight (g) | 74.4 ± 4.5 | 74.1 ± 5.2 | 64.0 ± 4.3 |
| Relative lung weight (% of body weight) | 2.71 ± 0.10 | 2.71 ± 0.16 | 2.80 ± 0.13 |
| Pulmonary water content (%) | 89.1 ± 0.2 | 89.1 ± 0.4 | 89.3 ± 0.4 |
| Pulmonary glycogen content (mg/g) | 2.50 ± 0.28 (n = 6) | 2.15 ± 0.16 | 2.54 ± 0.37 |
| Alveolar air-space (%) | 49.7 ± 2.8 | 54.8 ± 0.7 | 53.0 ± 3.5 |
| Alveolar tissue (%) | 44.4 ± 2.8 | 39.6 ± 0.8 | 38.7 ± 2.4 |
| Extra-alveolar air-space (%) | 2.7 ± 0.3 | 2.4 ± 0.2 | 3.0 ± 0.4 |
| Extra-alveolar tissue (%) | 3.2 ± 0.3 | 3.2 ± 0.3 | 5.4 ± 1.1 |
| Alveolar wall thickness (harmonic mean, μm) | 9.28 ± 0.66a | 7.15 ± 0.25b | 9.88 ± 0.43a |
| Alveolar surface area (cm2/cm3) | 707 ± 139 | 535 ± 143 | 911 ± 388 |
| Secondary septal crest density (number/mm2) | 404 ± 33 | 548 ± 65* | 527 ± 69 |
| Relative volume of elastin | |||
| Interalveolar septae | 28.9 ± 1.5a | 35.1 ± 1.8b | 31.4 ± 2.0a,b |
| Secondary septal crests | 11.2 ± 1.1 | 12.7 ± 1.4 | 11.6 ± 1.1 |
| Total | 40.2 ± 1.8a | 47.8 ± 2.6b | 43.0 ± 2.4a,b |
For each variable, values with different letters (a, b) are significantly different from each other, one-way ANOVA, P < .05.
Significantly different from saline group, unpaired Student's t test, P < .05.
Mean ± SEM Values for Animal Morphometry, Fetal Plasma Cortisol, and Lung Measurements in the 3 Treatment Groups
| . | Saline (n = 13) . | 0.5 LEP (n = 10) . | 1.0 LEP (n = 7) . |
|---|---|---|---|
| Maternal body weight (kg) | 44.8 ± 2.2 | 44.1 ± 2.4 | 45.6 ± 3.8 |
| Total placentome weight (g) | 319 ± 22 | 298 ± 18 | 267 ± 35 |
| Fetal body weight (kg) | 2.76 ± 0.16 | 2.74 ± 0.12 | 2.32 ± 0.19 |
| CRL (cm) | 43.0 ± 1.0 | 43.5 ± 0.7 | 41.4 ± 1.1 |
| BMI (kg/m2) | 14.8 ± 0.4 | 14.4 ± 0.5 | 13.3 ± 0.4 |
| PI (kg/m3) | 34.4 ± 0.9 | 33.2 ± 1.2 | 32.2 ± 0.5 |
| Plasma cortisol concentration (ng/mL) | |||
| Basal | 8.4 ± 1.2 | 6.6 ± 0.8 | 6.1 ± 1.4 |
| Day 5 | 10.4 ± 2.1 | 7.1 ± 1.3 | 12.0 ± 3.7 |
| Absolute lung weight (g) | 74.4 ± 4.5 | 74.1 ± 5.2 | 64.0 ± 4.3 |
| Relative lung weight (% of body weight) | 2.71 ± 0.10 | 2.71 ± 0.16 | 2.80 ± 0.13 |
| Pulmonary water content (%) | 89.1 ± 0.2 | 89.1 ± 0.4 | 89.3 ± 0.4 |
| Pulmonary glycogen content (mg/g) | 2.50 ± 0.28 (n = 6) | 2.15 ± 0.16 | 2.54 ± 0.37 |
| Alveolar air-space (%) | 49.7 ± 2.8 | 54.8 ± 0.7 | 53.0 ± 3.5 |
| Alveolar tissue (%) | 44.4 ± 2.8 | 39.6 ± 0.8 | 38.7 ± 2.4 |
| Extra-alveolar air-space (%) | 2.7 ± 0.3 | 2.4 ± 0.2 | 3.0 ± 0.4 |
| Extra-alveolar tissue (%) | 3.2 ± 0.3 | 3.2 ± 0.3 | 5.4 ± 1.1 |
| Alveolar wall thickness (harmonic mean, μm) | 9.28 ± 0.66a | 7.15 ± 0.25b | 9.88 ± 0.43a |
| Alveolar surface area (cm2/cm3) | 707 ± 139 | 535 ± 143 | 911 ± 388 |
| Secondary septal crest density (number/mm2) | 404 ± 33 | 548 ± 65* | 527 ± 69 |
| Relative volume of elastin | |||
| Interalveolar septae | 28.9 ± 1.5a | 35.1 ± 1.8b | 31.4 ± 2.0a,b |
| Secondary septal crests | 11.2 ± 1.1 | 12.7 ± 1.4 | 11.6 ± 1.1 |
| Total | 40.2 ± 1.8a | 47.8 ± 2.6b | 43.0 ± 2.4a,b |
| . | Saline (n = 13) . | 0.5 LEP (n = 10) . | 1.0 LEP (n = 7) . |
|---|---|---|---|
| Maternal body weight (kg) | 44.8 ± 2.2 | 44.1 ± 2.4 | 45.6 ± 3.8 |
| Total placentome weight (g) | 319 ± 22 | 298 ± 18 | 267 ± 35 |
| Fetal body weight (kg) | 2.76 ± 0.16 | 2.74 ± 0.12 | 2.32 ± 0.19 |
| CRL (cm) | 43.0 ± 1.0 | 43.5 ± 0.7 | 41.4 ± 1.1 |
| BMI (kg/m2) | 14.8 ± 0.4 | 14.4 ± 0.5 | 13.3 ± 0.4 |
| PI (kg/m3) | 34.4 ± 0.9 | 33.2 ± 1.2 | 32.2 ± 0.5 |
| Plasma cortisol concentration (ng/mL) | |||
| Basal | 8.4 ± 1.2 | 6.6 ± 0.8 | 6.1 ± 1.4 |
| Day 5 | 10.4 ± 2.1 | 7.1 ± 1.3 | 12.0 ± 3.7 |
| Absolute lung weight (g) | 74.4 ± 4.5 | 74.1 ± 5.2 | 64.0 ± 4.3 |
| Relative lung weight (% of body weight) | 2.71 ± 0.10 | 2.71 ± 0.16 | 2.80 ± 0.13 |
| Pulmonary water content (%) | 89.1 ± 0.2 | 89.1 ± 0.4 | 89.3 ± 0.4 |
| Pulmonary glycogen content (mg/g) | 2.50 ± 0.28 (n = 6) | 2.15 ± 0.16 | 2.54 ± 0.37 |
| Alveolar air-space (%) | 49.7 ± 2.8 | 54.8 ± 0.7 | 53.0 ± 3.5 |
| Alveolar tissue (%) | 44.4 ± 2.8 | 39.6 ± 0.8 | 38.7 ± 2.4 |
| Extra-alveolar air-space (%) | 2.7 ± 0.3 | 2.4 ± 0.2 | 3.0 ± 0.4 |
| Extra-alveolar tissue (%) | 3.2 ± 0.3 | 3.2 ± 0.3 | 5.4 ± 1.1 |
| Alveolar wall thickness (harmonic mean, μm) | 9.28 ± 0.66a | 7.15 ± 0.25b | 9.88 ± 0.43a |
| Alveolar surface area (cm2/cm3) | 707 ± 139 | 535 ± 143 | 911 ± 388 |
| Secondary septal crest density (number/mm2) | 404 ± 33 | 548 ± 65* | 527 ± 69 |
| Relative volume of elastin | |||
| Interalveolar septae | 28.9 ± 1.5a | 35.1 ± 1.8b | 31.4 ± 2.0a,b |
| Secondary septal crests | 11.2 ± 1.1 | 12.7 ± 1.4 | 11.6 ± 1.1 |
| Total | 40.2 ± 1.8a | 47.8 ± 2.6b | 43.0 ± 2.4a,b |
For each variable, values with different letters (a, b) are significantly different from each other, one-way ANOVA, P < .05.
Significantly different from saline group, unpaired Student's t test, P < .05.
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