Table 1 Open in new tab Primer and...

Gene

Forward Primer Sequence

Reverse Primer Sequence

Reporter Sequence

Reporter Dye

Leptin

CAAGACGATTGTCACCAGGATCAA

CCAGTGACCCTCTGTTTGGA

TCACACACGCAGTCCGT

FAM

Ob-Rb (long form)

GGAGACAGCCCTCTGTTAAATATGC

TGAGCTGTTTATAAGCCCTTGCT

CCTCCTCGGCTTCACC

FAM

Ob-R (all isoforms)

GAGCGCCCTTCTTACCTTTACTA

CCAACCGCTGTCAGAATTTTAGGT

CACAAGATGTCATATATTTTC

FAM

SP-A

GAGCCTGGCGAGAGAGG

CTTGATGTCTGATCTCATGGAGTGT

TCCTCCAGGGTTTCCAG

FAM

SP-B

AGCCTGGGCCTCAGACA

CGGCAGAGCCAGCAGAA

CAGCAGCGGTTCCCCA

FAM

SP-C

GCAGCAAAGAGGTCTTGATGGA

GATGAGAAGGCGTTTGATGTTCAC

CCGCCGGACTACTC

FAM

SP-D (bovine)

TaqMan(R) gene expression assays (assay ID, Bt03217554_mH; part number, 4 331 182)

FAM

Elastin

GCTGCAGCTGCTAAAGCA

GGCTCCTAGGACACCTCCAA

CCAGCGGCACCAAAT

FAM

VEGF-A

GCTCTCTTGGGTGCATTGGA

GCCTGGGACCACTTGGC

CCTTGCCTTGCTGCTCTACCTTCACCA

FAM

VEGF-R2

GAACTAGAATGAGAGCCCCTGATT

CCCCATGCCAGCAGTCAA

ATGGTCTGGTACATTTC

FAM

ACE

CCTTCCCGCTACAACTATGACT

GGACAACCGGAGGACAGATC

ATACTTGGTTCGAAGATACC

FAM

PPARγ

CGATTCCAGAAGTGCCTTGCT

GAGATCTCTGCTAACAGCTTTTCCT

CCAAACCTGATGGCATTAT

FAM

PTHrP (bovine)

TaqMan(R) gene expression assays (assay ID, Bt03224326_m1; part number, 4 351 372)

FAM

GAPDH

GCTACACTGAGGACCAGGTT

AGCATCGAAGGTAGAAGAGTGAGT

CTCCTGCGACTTCAAC

FAM

Cyclophilin A

GGTTCCTGCTTTCACAGAATAATTCC

GTACCATTATGGCGTGTGAAGTCA

CACCCTGGCACATAAA

FAM

Table 1

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Primer and Reporter Sequences Used for TaqMan qRT-PCR in the Sheep

Gene	Forward Primer Sequence	Reverse Primer Sequence	Reporter Sequence	Reporter Dye
Leptin	CAAGACGATTGTCACCAGGATCAA	CCAGTGACCCTCTGTTTGGA	TCACACACGCAGTCCGT	FAM
Ob-Rb (long form)	GGAGACAGCCCTCTGTTAAATATGC	TGAGCTGTTTATAAGCCCTTGCT	CCTCCTCGGCTTCACC	FAM
Ob-R (all isoforms)	GAGCGCCCTTCTTACCTTTACTA	CCAACCGCTGTCAGAATTTTAGGT	CACAAGATGTCATATATTTTC	FAM
SP-A	GAGCCTGGCGAGAGAGG	CTTGATGTCTGATCTCATGGAGTGT	TCCTCCAGGGTTTCCAG	FAM
SP-B	AGCCTGGGCCTCAGACA	CGGCAGAGCCAGCAGAA	CAGCAGCGGTTCCCCA	FAM
SP-C	GCAGCAAAGAGGTCTTGATGGA	GATGAGAAGGCGTTTGATGTTCAC	CCGCCGGACTACTC	FAM
SP-D (bovine)	TaqMan(R) gene expression assays (assay ID, Bt03217554_mH; part number, 4 331 182)			FAM
Elastin	GCTGCAGCTGCTAAAGCA	GGCTCCTAGGACACCTCCAA	CCAGCGGCACCAAAT	FAM
VEGF-A	GCTCTCTTGGGTGCATTGGA	GCCTGGGACCACTTGGC	CCTTGCCTTGCTGCTCTACCTTCACCA	FAM
VEGF-R2	GAACTAGAATGAGAGCCCCTGATT	CCCCATGCCAGCAGTCAA	ATGGTCTGGTACATTTC	FAM
ACE	CCTTCCCGCTACAACTATGACT	GGACAACCGGAGGACAGATC	ATACTTGGTTCGAAGATACC	FAM
PPARγ	CGATTCCAGAAGTGCCTTGCT	GAGATCTCTGCTAACAGCTTTTCCT	CCAAACCTGATGGCATTAT	FAM
PTHrP (bovine)	TaqMan(R) gene expression assays (assay ID, Bt03224326_m1; part number, 4 351 372)			FAM
GAPDH	GCTACACTGAGGACCAGGTT	AGCATCGAAGGTAGAAGAGTGAGT	CTCCTGCGACTTCAAC	FAM
Cyclophilin A	GGTTCCTGCTTTCACAGAATAATTCC	GTACCATTATGGCGTGTGAAGTCA	CACCCTGGCACATAAA	FAM

Gene	Forward Primer Sequence	Reverse Primer Sequence	Reporter Sequence	Reporter Dye
Leptin	CAAGACGATTGTCACCAGGATCAA	CCAGTGACCCTCTGTTTGGA	TCACACACGCAGTCCGT	FAM
Ob-Rb (long form)	GGAGACAGCCCTCTGTTAAATATGC	TGAGCTGTTTATAAGCCCTTGCT	CCTCCTCGGCTTCACC	FAM
Ob-R (all isoforms)	GAGCGCCCTTCTTACCTTTACTA	CCAACCGCTGTCAGAATTTTAGGT	CACAAGATGTCATATATTTTC	FAM
SP-A	GAGCCTGGCGAGAGAGG	CTTGATGTCTGATCTCATGGAGTGT	TCCTCCAGGGTTTCCAG	FAM
SP-B	AGCCTGGGCCTCAGACA	CGGCAGAGCCAGCAGAA	CAGCAGCGGTTCCCCA	FAM
SP-C	GCAGCAAAGAGGTCTTGATGGA	GATGAGAAGGCGTTTGATGTTCAC	CCGCCGGACTACTC	FAM
SP-D (bovine)	TaqMan(R) gene expression assays (assay ID, Bt03217554_mH; part number, 4 331 182)			FAM
Elastin	GCTGCAGCTGCTAAAGCA	GGCTCCTAGGACACCTCCAA	CCAGCGGCACCAAAT	FAM
VEGF-A	GCTCTCTTGGGTGCATTGGA	GCCTGGGACCACTTGGC	CCTTGCCTTGCTGCTCTACCTTCACCA	FAM
VEGF-R2	GAACTAGAATGAGAGCCCCTGATT	CCCCATGCCAGCAGTCAA	ATGGTCTGGTACATTTC	FAM
ACE	CCTTCCCGCTACAACTATGACT	GGACAACCGGAGGACAGATC	ATACTTGGTTCGAAGATACC	FAM
PPARγ	CGATTCCAGAAGTGCCTTGCT	GAGATCTCTGCTAACAGCTTTTCCT	CCAAACCTGATGGCATTAT	FAM
PTHrP (bovine)	TaqMan(R) gene expression assays (assay ID, Bt03224326_m1; part number, 4 351 372)			FAM
GAPDH	GCTACACTGAGGACCAGGTT	AGCATCGAAGGTAGAAGAGTGAGT	CTCCTGCGACTTCAAC	FAM
Cyclophilin A	GGTTCCTGCTTTCACAGAATAATTCC	GTACCATTATGGCGTGTGAAGTCA	CACCCTGGCACATAAA	FAM

Abbreviation: GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

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