3

Microbial community composition (percentages of total amount of clones for each sample) as determined by 16S rRNA gene clone libraries of biofilm formed in Mediterranean seawater at the different sampling times during the year. W1 and W2 represent biofilm age of 1 and 2 weeks, respectively, for each sampling experiment. (n.d., not detected). Numbers in parentheses represents number of clones analyzed for each library. Percent coverage was calculated for the 95% similarity level according to 1–(n/N) where n is the number of singeltons and N is the number of clones

Month, year and biofilm age
May 2008August 2008September 2008January 2009March 2009May 2009
PhylumClassW1 (67)W2 (63)W1 (83)W2 (90)W1 (62)W2 (71)W1 (63)W2 (30)W1 (82)W2 (76)W1 (83)W2 (57)
ProteobacteriaAlpha644878547544327372587576
Gamma121146320351041172
Delta13162n.d.675444
Bacteroidetes53511041713n.d.132455
Planctomycetes738915148n.d.232n.d.
Other bacteria9n.d.6164671031714
% coverage645663756873556076727069
Month, year and biofilm age
May 2008August 2008September 2008January 2009March 2009May 2009
PhylumClassW1 (67)W2 (63)W1 (83)W2 (90)W1 (62)W2 (71)W1 (63)W2 (30)W1 (82)W2 (76)W1 (83)W2 (57)
ProteobacteriaAlpha644878547544327372587576
Gamma121146320351041172
Delta13162n.d.675444
Bacteroidetes53511041713n.d.132455
Planctomycetes738915148n.d.232n.d.
Other bacteria9n.d.6164671031714
% coverage645663756873556076727069

Other bacteria = clones that were affiliated with various other bacterial phyla in which their overall percentage in the community was below 4%. Some of these groups appeared only once in the entire year. These bacterial groups included Betaproteobacteria, Acidobacteria, Chloroflexi, Fusobacteria, Verrucomicrobia, Firmicutes and bacteria affiliated with candidate divisions TM6, OD1 and OP3.

3

Microbial community composition (percentages of total amount of clones for each sample) as determined by 16S rRNA gene clone libraries of biofilm formed in Mediterranean seawater at the different sampling times during the year. W1 and W2 represent biofilm age of 1 and 2 weeks, respectively, for each sampling experiment. (n.d., not detected). Numbers in parentheses represents number of clones analyzed for each library. Percent coverage was calculated for the 95% similarity level according to 1–(n/N) where n is the number of singeltons and N is the number of clones

Month, year and biofilm age
May 2008August 2008September 2008January 2009March 2009May 2009
PhylumClassW1 (67)W2 (63)W1 (83)W2 (90)W1 (62)W2 (71)W1 (63)W2 (30)W1 (82)W2 (76)W1 (83)W2 (57)
ProteobacteriaAlpha644878547544327372587576
Gamma121146320351041172
Delta13162n.d.675444
Bacteroidetes53511041713n.d.132455
Planctomycetes738915148n.d.232n.d.
Other bacteria9n.d.6164671031714
% coverage645663756873556076727069
Month, year and biofilm age
May 2008August 2008September 2008January 2009March 2009May 2009
PhylumClassW1 (67)W2 (63)W1 (83)W2 (90)W1 (62)W2 (71)W1 (63)W2 (30)W1 (82)W2 (76)W1 (83)W2 (57)
ProteobacteriaAlpha644878547544327372587576
Gamma121146320351041172
Delta13162n.d.675444
Bacteroidetes53511041713n.d.132455
Planctomycetes738915148n.d.232n.d.
Other bacteria9n.d.6164671031714
% coverage645663756873556076727069

Other bacteria = clones that were affiliated with various other bacterial phyla in which their overall percentage in the community was below 4%. Some of these groups appeared only once in the entire year. These bacterial groups included Betaproteobacteria, Acidobacteria, Chloroflexi, Fusobacteria, Verrucomicrobia, Firmicutes and bacteria affiliated with candidate divisions TM6, OD1 and OP3.

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