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Microbial community composition of processed seawater as determined by 16S rRNA gene clone libraries. The average (± SD) percentages of three sampling dates in each month are presented. (n.d., not detected). Numbers in parentheses represent number of clones analyzed for each library. Percent coverage was calculated for the 95% similarity level according to 1–(n/N) where n is the number of singeltons and N is the number of clones

Sampling month and year
PhylumClassMay 2008 (217)August 2008 (418)September 2008 (215)January 2009 (145)March 2009 (199)May 2009 (198)
ProteobacteriaAlpha48 ± 426 ± 234 ± 1441 ± 1647 ± 935 ± 8
Gamma8 ± 47 ± 27 ± 46 ± 22 ± 25 ± 4
Delta1 ± 0.54 ± 23 ± 23 ± 2n.d1 ± 1
Bacteroidetes15 ± 59 ± 26 ± 218 ± 623 ± 39 ± 3
Planctomycetes3 ± 34 ± 23 ± 21 ± 12 ± 2n.d
Actinobacteria8 ± 517 ± 325 ± 1212 ± 822 ± 1135 ± 9
Cyanobacteria15 ± 727 ± 211 ± 49 ± 51 ± 111 ± 6
Other bacteria2 ± 16 ± 311 ± 410 ± 53 ± 14 ± 3
% coverage616062587772
Sampling month and year
PhylumClassMay 2008 (217)August 2008 (418)September 2008 (215)January 2009 (145)March 2009 (199)May 2009 (198)
ProteobacteriaAlpha48 ± 426 ± 234 ± 1441 ± 1647 ± 935 ± 8
Gamma8 ± 47 ± 27 ± 46 ± 22 ± 25 ± 4
Delta1 ± 0.54 ± 23 ± 23 ± 2n.d1 ± 1
Bacteroidetes15 ± 59 ± 26 ± 218 ± 623 ± 39 ± 3
Planctomycetes3 ± 34 ± 23 ± 21 ± 12 ± 2n.d
Actinobacteria8 ± 517 ± 325 ± 1212 ± 822 ± 1135 ± 9
Cyanobacteria15 ± 727 ± 211 ± 49 ± 51 ± 111 ± 6
Other bacteria2 ± 16 ± 311 ± 410 ± 53 ± 14 ± 3
% coverage616062587772

Other bacteria = clones that were affiliated with various other bacterial phyla which their overall percentage in the community was below 4%. Some of these groups appeared only once in the entire year. These bacterial groups included Betaproteobacteria, Acidobacteria, Chloroflexi, Fusobacteria, Verrucomicrobia, Firmicutes and bacteria affiliated with candidate divisions TM6, OD1 and OP3.

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Microbial community composition of processed seawater as determined by 16S rRNA gene clone libraries. The average (± SD) percentages of three sampling dates in each month are presented. (n.d., not detected). Numbers in parentheses represent number of clones analyzed for each library. Percent coverage was calculated for the 95% similarity level according to 1–(n/N) where n is the number of singeltons and N is the number of clones

Sampling month and year
PhylumClassMay 2008 (217)August 2008 (418)September 2008 (215)January 2009 (145)March 2009 (199)May 2009 (198)
ProteobacteriaAlpha48 ± 426 ± 234 ± 1441 ± 1647 ± 935 ± 8
Gamma8 ± 47 ± 27 ± 46 ± 22 ± 25 ± 4
Delta1 ± 0.54 ± 23 ± 23 ± 2n.d1 ± 1
Bacteroidetes15 ± 59 ± 26 ± 218 ± 623 ± 39 ± 3
Planctomycetes3 ± 34 ± 23 ± 21 ± 12 ± 2n.d
Actinobacteria8 ± 517 ± 325 ± 1212 ± 822 ± 1135 ± 9
Cyanobacteria15 ± 727 ± 211 ± 49 ± 51 ± 111 ± 6
Other bacteria2 ± 16 ± 311 ± 410 ± 53 ± 14 ± 3
% coverage616062587772
Sampling month and year
PhylumClassMay 2008 (217)August 2008 (418)September 2008 (215)January 2009 (145)March 2009 (199)May 2009 (198)
ProteobacteriaAlpha48 ± 426 ± 234 ± 1441 ± 1647 ± 935 ± 8
Gamma8 ± 47 ± 27 ± 46 ± 22 ± 25 ± 4
Delta1 ± 0.54 ± 23 ± 23 ± 2n.d1 ± 1
Bacteroidetes15 ± 59 ± 26 ± 218 ± 623 ± 39 ± 3
Planctomycetes3 ± 34 ± 23 ± 21 ± 12 ± 2n.d
Actinobacteria8 ± 517 ± 325 ± 1212 ± 822 ± 1135 ± 9
Cyanobacteria15 ± 727 ± 211 ± 49 ± 51 ± 111 ± 6
Other bacteria2 ± 16 ± 311 ± 410 ± 53 ± 14 ± 3
% coverage616062587772

Other bacteria = clones that were affiliated with various other bacterial phyla which their overall percentage in the community was below 4%. Some of these groups appeared only once in the entire year. These bacterial groups included Betaproteobacteria, Acidobacteria, Chloroflexi, Fusobacteria, Verrucomicrobia, Firmicutes and bacteria affiliated with candidate divisions TM6, OD1 and OP3.

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