2 Open in new tab The richness,...

Sample	Richness (S)	Diversity (H)	Evenness (E)
V1, V3, and V1+V2 domains
NSB 0–5 cm	20.33 (± 1.15)^a,b	2.40 (± 0.05)^c,d	0.80 (± 0.01)^f
NSB 5–15 cm	25.67 (± 3.78)^a,b	2.63 (± 0.18)^c	0.81 (± 0.04)^f
NSB 15–30 cm	24.67(± 0.57)^a,b	2.68 (± 0.12)^e	0.84 (± 0.03)^f
IMP 0–30 cm	19.33 (± 1.52)^a	2.26 (± 0.11)^c,d	0.76 (± 0.02)^f,g
ANOVA	F=6.42, P<0.02	F= 7.52, P<0.01	F= 4.63, P<0.04
V1, V3 domains
NSB 0–5 cm	12.89 (± 1.76)^h	2.03 (± 0.14)^i,j	0.80 (± 0.04)^l
NSB 5–15 cm	14.11 (± 2.31)^h	2.16 (± 0.12)^i,j	0.82 (± 0.03)^l
NSB 15–30 cm	14.78 (± 0.83)^h	2.25 (± 0.10)^j	0.83 (± 0.03)^l
IMP 0–30 cm	14.00 (± 0.50)^h	1.85 (± 0.07)^k	0.70 (± 0.03)^m
ANOVA	F=2. 34, P<0.09	F=21.52, P<0.01	F=28.58, P<0.01
V1, V1+V2 domains
NSB 0–5 cm	17.67 (± 1.80)ⁿ	2.32 (± 0.08)^p	0.81(± 0.02)^s
NSB 5–15 cm	21.89 (± 2.14)^o	2.52 (± 0.15)^q	0.81(± 0.03)^s
NSB 15–30 cm	18.33 (± 1.11)ⁿ	2.33 (± 0.08)^p	0.80 (± 0.02)^s
IMP 0–30 cm	18.89 (± 0.92)ⁿ	2.13 (± 0.09)^r	0.73 (± 0.03)^t
ANOVA	F= 12.55, P<0.01	F= 19.11, P<0.01	F= 22.25, P<0.01
V1+V2, V3 domains
NSB 0–5 cm	9.33 (± 0.57)^u	2.10 (± 0.03)^w	0.94 (± 0.01)^y
NSB 5–15 cm	15.67 (± 3.05)^v	2.51 (± 0.27)^w,x	0.91 (± 0.05)^y
NSB 15–30 cm	12.67 (± 0.57)^u,v	2.44 (± 0.08)^w,x	0.96 (± 0.02)^y
IMP 0–30 cm	15.00 (± 0.00)^v	2.56 (± 0.01)^x	0.95 (± 0.01)^y
ANOVA	F=9.82, P<0.01	F=6.21, P<0.02	F=1.79, P<0.23

Sample

Richness (S)

Diversity (H)

Evenness (E)

V1, V3, and V1+V2 domains

NSB 0–5 cm

20.33 (± 1.15)^a,b

2.40 (± 0.05)^c,d

0.80 (± 0.01)^f

NSB 5–15 cm

25.67 (± 3.78)^a,b

2.63 (± 0.18)^c

0.81 (± 0.04)^f

NSB 15–30 cm

24.67(± 0.57)^a,b

2.68 (± 0.12)^e

0.84 (± 0.03)^f

IMP 0–30 cm

19.33 (± 1.52)^a

2.26 (± 0.11)^c,d

0.76 (± 0.02)^f,g

ANOVA

F=6.42, P<0.02

F= 7.52, P<0.01

F= 4.63, P<0.04

V1, V3 domains

NSB 0–5 cm

12.89 (± 1.76)^h

2.03 (± 0.14)^i,j

0.80 (± 0.04)^l

NSB 5–15 cm

14.11 (± 2.31)^h

2.16 (± 0.12)^i,j

0.82 (± 0.03)^l

NSB 15–30 cm

14.78 (± 0.83)^h

2.25 (± 0.10)^j

0.83 (± 0.03)^l

IMP 0–30 cm

14.00 (± 0.50)^h

1.85 (± 0.07)^k

0.70 (± 0.03)^m

ANOVA

F=2. 34, P<0.09

F=21.52, P<0.01

F=28.58, P<0.01

V1, V1+V2 domains

NSB 0–5 cm

17.67 (± 1.80)ⁿ

2.32 (± 0.08)^p

0.81(± 0.02)^s

NSB 5–15 cm

21.89 (± 2.14)^o

2.52 (± 0.15)^q

0.81(± 0.03)^s

NSB 15–30 cm

18.33 (± 1.11)ⁿ

2.33 (± 0.08)^p

0.80 (± 0.02)^s

IMP 0–30 cm

18.89 (± 0.92)ⁿ

2.13 (± 0.09)^r

0.73 (± 0.03)^t

ANOVA

F= 12.55, P<0.01

F= 19.11, P<0.01

F= 22.25, P<0.01

V1+V2, V3 domains

NSB 0–5 cm

9.33 (± 0.57)^u

2.10 (± 0.03)^w

0.94 (± 0.01)^y

NSB 5–15 cm

15.67 (± 3.05)^v

2.51 (± 0.27)^w,x

0.91 (± 0.05)^y

NSB 15–30 cm

12.67 (± 0.57)^u,v

2.44 (± 0.08)^w,x

0.96 (± 0.02)^y

IMP 0–30 cm

15.00 (± 0.00)^v

2.56 (± 0.01)^x

0.95 (± 0.01)^y

ANOVA

F=9.82, P<0.01

F=6.21, P<0.02

F=1.79, P<0.23

Sample	Richness (S)	Diversity (H)	Evenness (E)
V1, V3, and V1+V2 domains
NSB 0–5 cm	20.33 (± 1.15)^a,b	2.40 (± 0.05)^c,d	0.80 (± 0.01)^f
NSB 5–15 cm	25.67 (± 3.78)^a,b	2.63 (± 0.18)^c	0.81 (± 0.04)^f
NSB 15–30 cm	24.67(± 0.57)^a,b	2.68 (± 0.12)^e	0.84 (± 0.03)^f
IMP 0–30 cm	19.33 (± 1.52)^a	2.26 (± 0.11)^c,d	0.76 (± 0.02)^f,g
ANOVA	F=6.42, P<0.02	F= 7.52, P<0.01	F= 4.63, P<0.04
V1, V3 domains
NSB 0–5 cm	12.89 (± 1.76)^h	2.03 (± 0.14)^i,j	0.80 (± 0.04)^l
NSB 5–15 cm	14.11 (± 2.31)^h	2.16 (± 0.12)^i,j	0.82 (± 0.03)^l
NSB 15–30 cm	14.78 (± 0.83)^h	2.25 (± 0.10)^j	0.83 (± 0.03)^l
IMP 0–30 cm	14.00 (± 0.50)^h	1.85 (± 0.07)^k	0.70 (± 0.03)^m
ANOVA	F=2. 34, P<0.09	F=21.52, P<0.01	F=28.58, P<0.01
V1, V1+V2 domains
NSB 0–5 cm	17.67 (± 1.80)ⁿ	2.32 (± 0.08)^p	0.81(± 0.02)^s
NSB 5–15 cm	21.89 (± 2.14)^o	2.52 (± 0.15)^q	0.81(± 0.03)^s
NSB 15–30 cm	18.33 (± 1.11)ⁿ	2.33 (± 0.08)^p	0.80 (± 0.02)^s
IMP 0–30 cm	18.89 (± 0.92)ⁿ	2.13 (± 0.09)^r	0.73 (± 0.03)^t
ANOVA	F= 12.55, P<0.01	F= 19.11, P<0.01	F= 22.25, P<0.01
V1+V2, V3 domains
NSB 0–5 cm	9.33 (± 0.57)^u	2.10 (± 0.03)^w	0.94 (± 0.01)^y
NSB 5–15 cm	15.67 (± 3.05)^v	2.51 (± 0.27)^w,x	0.91 (± 0.05)^y
NSB 15–30 cm	12.67 (± 0.57)^u,v	2.44 (± 0.08)^w,x	0.96 (± 0.02)^y
IMP 0–30 cm	15.00 (± 0.00)^v	2.56 (± 0.01)^x	0.95 (± 0.01)^y
ANOVA	F=9.82, P<0.01	F=6.21, P<0.02	F=1.79, P<0.23

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The richness, diversity, and evenness mean values for various combinations of the domain data sets produced using amplicon length heterogeneity PCR profiling

Sample	Richness (S)	Diversity (H)	Evenness (E)
V1, V3, and V1+V2 domains
NSB 0–5 cm	20.33 (± 1.15)^a,b	2.40 (± 0.05)^c,d	0.80 (± 0.01)^f
NSB 5–15 cm	25.67 (± 3.78)^a,b	2.63 (± 0.18)^c	0.81 (± 0.04)^f
NSB 15–30 cm	24.67(± 0.57)^a,b	2.68 (± 0.12)^e	0.84 (± 0.03)^f
IMP 0–30 cm	19.33 (± 1.52)^a	2.26 (± 0.11)^c,d	0.76 (± 0.02)^f,g
ANOVA	F=6.42, P<0.02	F= 7.52, P<0.01	F= 4.63, P<0.04
V1, V3 domains
NSB 0–5 cm	12.89 (± 1.76)^h	2.03 (± 0.14)^i,j	0.80 (± 0.04)^l
NSB 5–15 cm	14.11 (± 2.31)^h	2.16 (± 0.12)^i,j	0.82 (± 0.03)^l
NSB 15–30 cm	14.78 (± 0.83)^h	2.25 (± 0.10)^j	0.83 (± 0.03)^l
IMP 0–30 cm	14.00 (± 0.50)^h	1.85 (± 0.07)^k	0.70 (± 0.03)^m
ANOVA	F=2. 34, P<0.09	F=21.52, P<0.01	F=28.58, P<0.01
V1, V1+V2 domains
NSB 0–5 cm	17.67 (± 1.80)ⁿ	2.32 (± 0.08)^p	0.81(± 0.02)^s
NSB 5–15 cm	21.89 (± 2.14)^o	2.52 (± 0.15)^q	0.81(± 0.03)^s
NSB 15–30 cm	18.33 (± 1.11)ⁿ	2.33 (± 0.08)^p	0.80 (± 0.02)^s
IMP 0–30 cm	18.89 (± 0.92)ⁿ	2.13 (± 0.09)^r	0.73 (± 0.03)^t
ANOVA	F= 12.55, P<0.01	F= 19.11, P<0.01	F= 22.25, P<0.01
V1+V2, V3 domains
NSB 0–5 cm	9.33 (± 0.57)^u	2.10 (± 0.03)^w	0.94 (± 0.01)^y
NSB 5–15 cm	15.67 (± 3.05)^v	2.51 (± 0.27)^w,x	0.91 (± 0.05)^y
NSB 15–30 cm	12.67 (± 0.57)^u,v	2.44 (± 0.08)^w,x	0.96 (± 0.02)^y
IMP 0–30 cm	15.00 (± 0.00)^v	2.56 (± 0.01)^x	0.95 (± 0.01)^y
ANOVA	F=9.82, P<0.01	F=6.21, P<0.02	F=1.79, P<0.23

Sample	Richness (S)	Diversity (H)	Evenness (E)
V1, V3, and V1+V2 domains
NSB 0–5 cm	20.33 (± 1.15)^a,b	2.40 (± 0.05)^c,d	0.80 (± 0.01)^f
NSB 5–15 cm	25.67 (± 3.78)^a,b	2.63 (± 0.18)^c	0.81 (± 0.04)^f
NSB 15–30 cm	24.67(± 0.57)^a,b	2.68 (± 0.12)^e	0.84 (± 0.03)^f
IMP 0–30 cm	19.33 (± 1.52)^a	2.26 (± 0.11)^c,d	0.76 (± 0.02)^f,g
ANOVA	F=6.42, P<0.02	F= 7.52, P<0.01	F= 4.63, P<0.04
V1, V3 domains
NSB 0–5 cm	12.89 (± 1.76)^h	2.03 (± 0.14)^i,j	0.80 (± 0.04)^l
NSB 5–15 cm	14.11 (± 2.31)^h	2.16 (± 0.12)^i,j	0.82 (± 0.03)^l
NSB 15–30 cm	14.78 (± 0.83)^h	2.25 (± 0.10)^j	0.83 (± 0.03)^l
IMP 0–30 cm	14.00 (± 0.50)^h	1.85 (± 0.07)^k	0.70 (± 0.03)^m
ANOVA	F=2. 34, P<0.09	F=21.52, P<0.01	F=28.58, P<0.01
V1, V1+V2 domains
NSB 0–5 cm	17.67 (± 1.80)ⁿ	2.32 (± 0.08)^p	0.81(± 0.02)^s
NSB 5–15 cm	21.89 (± 2.14)^o	2.52 (± 0.15)^q	0.81(± 0.03)^s
NSB 15–30 cm	18.33 (± 1.11)ⁿ	2.33 (± 0.08)^p	0.80 (± 0.02)^s
IMP 0–30 cm	18.89 (± 0.92)ⁿ	2.13 (± 0.09)^r	0.73 (± 0.03)^t
ANOVA	F= 12.55, P<0.01	F= 19.11, P<0.01	F= 22.25, P<0.01
V1+V2, V3 domains
NSB 0–5 cm	9.33 (± 0.57)^u	2.10 (± 0.03)^w	0.94 (± 0.01)^y
NSB 5–15 cm	15.67 (± 3.05)^v	2.51 (± 0.27)^w,x	0.91 (± 0.05)^y
NSB 15–30 cm	12.67 (± 0.57)^u,v	2.44 (± 0.08)^w,x	0.96 (± 0.02)^y
IMP 0–30 cm	15.00 (± 0.00)^v	2.56 (± 0.01)^x	0.95 (± 0.01)^y
ANOVA	F=9.82, P<0.01	F=6.21, P<0.02	F=1.79, P<0.23

Richness (S)=no. of peaks in each sample; diversity (H)=−Σ(p_i)ln(p_i), where p_iis the relative ratio of individual peak heights; evenness (E)=H/H_max, where H_max=ln(S); numbers in parentheses are±SD of the calculated means (n=9). Within each domain set, indices followed by the same letter are not significantly different from each other using Bonferroni post-hoc comparisons, P<0.05.

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