Additions used in final culturing media for each of the two mixed cultures and for strain TR1 (final concentrations)
PM1 contains the following final concentrations of minerals (in mg L−1): 1.5 KCl, 13.6 KH2PO4, 26.8 NH4Cl, 0.024 CoCl2·6H2O, 0.075 ZnCl2, 0.019 H3BO3, 0.024 NiCl2× 6H2O, 0.024 Na2Mo4·2H2O, 1.344 FeCl2·4H2O, 0.026 MnSO4·4H2O, 1.556 MgSO4, 2.336 CaCl2·2H2O, 0.009 CuSO4·5H2O, 3.446 AlK(SO4)2·12H2O.
83 mM Ti (III) nitrilotriacetate (NTA) or citrate stock solutions were made by combining the following anaerobic solutions and filter sterilizing in an anaerobic glove box: 4.8 mL of 0.5 M sodium citrate or sodium nitrilotriacetate, 0.55 mL of Ti (III) chloride (15% solution in HCl; Riedel-de Haen, Seelze, Germany), and 7.2 mL of one of the following: 1 M Tris buffer (pH 8) for a pH 7 solution, 0.4 M solution for a pH 5 solution of Ti (III) nitrilotriacetate, or 0.12 M NaOH for a pH 5 solution of Ti (III) citrate.
The stock solution of vitamins contains the following (in mg L−1): 20 biotin, 20 folic acid, 100 pyridoxine hydrochloride, 50 thiamine hydrochloride, 50 nicotinic acid, 50 dl-calcium pantothenate, 1 vitamin B12, 50 p-aminobenzoic acid, 50 lipoic acid. Note: this is a 10-fold increase from that used by Balch et al., (1979).
The stock solution of FAM contains 7.5 mM NaOH and 2.5 mM each of isobutyric, valeric, isovaleric and dl-2(a)-methylbutyric acid.
Additions used in final culturing media for each of the two mixed cultures and for strain TR1 (final concentrations)
PM1 contains the following final concentrations of minerals (in mg L−1): 1.5 KCl, 13.6 KH2PO4, 26.8 NH4Cl, 0.024 CoCl2·6H2O, 0.075 ZnCl2, 0.019 H3BO3, 0.024 NiCl2× 6H2O, 0.024 Na2Mo4·2H2O, 1.344 FeCl2·4H2O, 0.026 MnSO4·4H2O, 1.556 MgSO4, 2.336 CaCl2·2H2O, 0.009 CuSO4·5H2O, 3.446 AlK(SO4)2·12H2O.
83 mM Ti (III) nitrilotriacetate (NTA) or citrate stock solutions were made by combining the following anaerobic solutions and filter sterilizing in an anaerobic glove box: 4.8 mL of 0.5 M sodium citrate or sodium nitrilotriacetate, 0.55 mL of Ti (III) chloride (15% solution in HCl; Riedel-de Haen, Seelze, Germany), and 7.2 mL of one of the following: 1 M Tris buffer (pH 8) for a pH 7 solution, 0.4 M solution for a pH 5 solution of Ti (III) nitrilotriacetate, or 0.12 M NaOH for a pH 5 solution of Ti (III) citrate.
The stock solution of vitamins contains the following (in mg L−1): 20 biotin, 20 folic acid, 100 pyridoxine hydrochloride, 50 thiamine hydrochloride, 50 nicotinic acid, 50 dl-calcium pantothenate, 1 vitamin B12, 50 p-aminobenzoic acid, 50 lipoic acid. Note: this is a 10-fold increase from that used by Balch et al., (1979).
The stock solution of FAM contains 7.5 mM NaOH and 2.5 mM each of isobutyric, valeric, isovaleric and dl-2(a)-methylbutyric acid.
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