Open in new tab Comparison of growth...

Culture	Growth rate (h gen⁻¹)	Concentrations produced (mM)	O/R index	% C recovery
WC1	1.86	8.30	3.80	7.12	6.60	13.95	3.92	1.04	99.14
WC2	2.50	2.05	17.00	3.03	1.73	6.59	3.82	0.97	102.64
WC3	2.32	3.10	16.23	4.49	2.10	8.22	3.78	0.98	108.95
WC4	6.74	7.11	8.22	7.96	4.82	13.38	3.96	1.00	107.14
WC5	2.16	4.12	13.06	3.64	1.75	7.02	3.79	0.95	109.43
WC6	2.28	3.62	15.10	2.82	1.56	6.09	3.81	0.94	110.88
WC7	2.40	2.01	14.89	3.10	1.78	6.42	3.81	0.94	107.11
WC8	2.65	6.80	13.53	4.94	2.88	9.98	3.77	1.08	115.39
WC9	3.01	2.85	14.17	2.84	2.10	6.11	3.66	0.92	111.59
WC10	2.62	2.84	12.83	2.78	2.52	6.83	3.82	0.99	97.34
WC11	1.88	2.46	11.89	2.21	1.39	5.40	3.85	0.93	101.17
WC12	2.45	0.98	16.52	3.94	2.23	7.23	3.84	0.91	109.49
T. brockii ssp. brockii	2.68	2.26	11.92	10.83	0.66	11.84	3.68	0.98	107.05
T. pseudethanolicus	2.14	6.12	4.74	12.23	0.98	15.62	3.77	1.00	107.95
T. thermohydrosulfuricus	5.57	9.33	4.03	7.63	6.88	13.09	3.96	0.93	96.67

Culture	Growth rate (h gen⁻¹)	Concentrations produced (mM)	O/R index	% C recovery
WC1	1.86	8.30	3.80	7.12	6.60	13.95	3.92	1.04	99.14
WC2	2.50	2.05	17.00	3.03	1.73	6.59	3.82	0.97	102.64
WC3	2.32	3.10	16.23	4.49	2.10	8.22	3.78	0.98	108.95
WC4	6.74	7.11	8.22	7.96	4.82	13.38	3.96	1.00	107.14
WC5	2.16	4.12	13.06	3.64	1.75	7.02	3.79	0.95	109.43
WC6	2.28	3.62	15.10	2.82	1.56	6.09	3.81	0.94	110.88
WC7	2.40	2.01	14.89	3.10	1.78	6.42	3.81	0.94	107.11
WC8	2.65	6.80	13.53	4.94	2.88	9.98	3.77	1.08	115.39
WC9	3.01	2.85	14.17	2.84	2.10	6.11	3.66	0.92	111.59
WC10	2.62	2.84	12.83	2.78	2.52	6.83	3.82	0.99	97.34
WC11	1.88	2.46	11.89	2.21	1.39	5.40	3.85	0.93	101.17
WC12	2.45	0.98	16.52	3.94	2.23	7.23	3.84	0.91	109.49
T. brockii ssp. brockii	2.68	2.26	11.92	10.83	0.66	11.84	3.68	0.98	107.05
T. pseudethanolicus	2.14	6.12	4.74	12.23	0.98	15.62	3.77	1.00	107.95
T. thermohydrosulfuricus	5.57	9.33	4.03	7.63	6.88	13.09	3.96	0.93	96.67

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Comparison of growth rates and fermentation end product averages on 2 g L⁻¹ cellobiose at 60 °C among isolates WC1–WC12, Thermoanaerobacter brockii ssp. brockii DSM 1457, Thermoanaerobacter pseudethanolicus DSM 2355, and Thermoanaerobacter thermohydrosulfuricus DSM 567

Culture	Growth rate (h gen⁻¹)	Concentrations produced (mM)						O/R index	% C recovery
Acetate	Lactate	Ethanol	H₂	CO₂	Biomass
WC1	1.86	8.30	3.80	7.12	6.60	13.95	3.92	1.04	99.14
WC2	2.50	2.05	17.00	3.03	1.73	6.59	3.82	0.97	102.64
WC3	2.32	3.10	16.23	4.49	2.10	8.22	3.78	0.98	108.95
WC4	6.74	7.11	8.22	7.96	4.82	13.38	3.96	1.00	107.14
WC5	2.16	4.12	13.06	3.64	1.75	7.02	3.79	0.95	109.43
WC6	2.28	3.62	15.10	2.82	1.56	6.09	3.81	0.94	110.88
WC7	2.40	2.01	14.89	3.10	1.78	6.42	3.81	0.94	107.11
WC8	2.65	6.80	13.53	4.94	2.88	9.98	3.77	1.08	115.39
WC9	3.01	2.85	14.17	2.84	2.10	6.11	3.66	0.92	111.59
WC10	2.62	2.84	12.83	2.78	2.52	6.83	3.82	0.99	97.34
WC11	1.88	2.46	11.89	2.21	1.39	5.40	3.85	0.93	101.17
WC12	2.45	0.98	16.52	3.94	2.23	7.23	3.84	0.91	109.49
T. brockii ssp. brockii	2.68	2.26	11.92	10.83	0.66	11.84	3.68	0.98	107.05
T. pseudethanolicus	2.14	6.12	4.74	12.23	0.98	15.62	3.77	1.00	107.95
T. thermohydrosulfuricus	5.57	9.33	4.03	7.63	6.88	13.09	3.96	0.93	96.67

Culture	Growth rate (h gen⁻¹)	Concentrations produced (mM)						O/R index	% C recovery
Acetate	Lactate	Ethanol	H₂	CO₂	Biomass
WC1	1.86	8.30	3.80	7.12	6.60	13.95	3.92	1.04	99.14
WC2	2.50	2.05	17.00	3.03	1.73	6.59	3.82	0.97	102.64
WC3	2.32	3.10	16.23	4.49	2.10	8.22	3.78	0.98	108.95
WC4	6.74	7.11	8.22	7.96	4.82	13.38	3.96	1.00	107.14
WC5	2.16	4.12	13.06	3.64	1.75	7.02	3.79	0.95	109.43
WC6	2.28	3.62	15.10	2.82	1.56	6.09	3.81	0.94	110.88
WC7	2.40	2.01	14.89	3.10	1.78	6.42	3.81	0.94	107.11
WC8	2.65	6.80	13.53	4.94	2.88	9.98	3.77	1.08	115.39
WC9	3.01	2.85	14.17	2.84	2.10	6.11	3.66	0.92	111.59
WC10	2.62	2.84	12.83	2.78	2.52	6.83	3.82	0.99	97.34
WC11	1.88	2.46	11.89	2.21	1.39	5.40	3.85	0.93	101.17
WC12	2.45	0.98	16.52	3.94	2.23	7.23	3.84	0.91	109.49
T. brockii ssp. brockii	2.68	2.26	11.92	10.83	0.66	11.84	3.68	0.98	107.05
T. pseudethanolicus	2.14	6.12	4.74	12.23	0.98	15.62	3.77	1.00	107.95
T. thermohydrosulfuricus	5.57	9.33	4.03	7.63	6.88	13.09	3.96	0.93	96.67

End product data correspond to measurements taken at an average OD₆₀₀ = 0.75 ± 0.03 and corrected for carryover from inoculation. Values represent averages from three independent experiments using three biologic replicates/experiment.

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