Values for single mutants constructed by point mutagenesis and compared to multiple mutants found during the screening
| . | . | UAA . | UAG . | UGA . | |||
|---|---|---|---|---|---|---|---|
| . | . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . |
| D96E | 7.3 | ×2 | 10.7 | ×2 | 12.3 | ×2 | |
| A2 | E360G | 4.8 | ×3 | 11.9 | ×3 | 6.9 | ×3 |
| A8 | E104K | 3.6 | ×5 | 10.4 | ×3 | 3.9 | ×6 |
| EE/KG | E104K, E360G | 3.2 | ×5 | 12.9 | ×3 | 5 | ×4 |
| B1 | Q76R | 3.7 | ×4 | 9.5 | ×4 | 5.9 | ×4 |
| QE/RG | Q76R, E360G | 2.4 | ×7 | 9.2 | ×4 | 2.2 | ×10 |
| T50K | 2.9 | ×6 | 5.3 | ×6 | 3.8 | ×6 | |
| C11 | N58K | 2.8 | ×6 | 7.9 | ×4 | 4.7 | ×5 |
| NE/KG | N58K, E360G | 2.2 | ×7 | 5.6 | ×6 | 2.7 | ×8 |
| . | . | UAA . | UAG . | UGA . | |||
|---|---|---|---|---|---|---|---|
| . | . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . |
| D96E | 7.3 | ×2 | 10.7 | ×2 | 12.3 | ×2 | |
| A2 | E360G | 4.8 | ×3 | 11.9 | ×3 | 6.9 | ×3 |
| A8 | E104K | 3.6 | ×5 | 10.4 | ×3 | 3.9 | ×6 |
| EE/KG | E104K, E360G | 3.2 | ×5 | 12.9 | ×3 | 5 | ×4 |
| B1 | Q76R | 3.7 | ×4 | 9.5 | ×4 | 5.9 | ×4 |
| QE/RG | Q76R, E360G | 2.4 | ×7 | 9.2 | ×4 | 2.2 | ×10 |
| T50K | 2.9 | ×6 | 5.3 | ×6 | 3.8 | ×6 | |
| C11 | N58K | 2.8 | ×6 | 7.9 | ×4 | 4.7 | ×5 |
| NE/KG | N58K, E360G | 2.2 | ×7 | 5.6 | ×6 | 2.7 | ×8 |
Same legend as for Table 2. The name of the allele is indicated in the left column for the mutants identified during the screening, and for the double mutants.
Values for single mutants constructed by point mutagenesis and compared to multiple mutants found during the screening
| . | . | UAA . | UAG . | UGA . | |||
|---|---|---|---|---|---|---|---|
| . | . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . |
| D96E | 7.3 | ×2 | 10.7 | ×2 | 12.3 | ×2 | |
| A2 | E360G | 4.8 | ×3 | 11.9 | ×3 | 6.9 | ×3 |
| A8 | E104K | 3.6 | ×5 | 10.4 | ×3 | 3.9 | ×6 |
| EE/KG | E104K, E360G | 3.2 | ×5 | 12.9 | ×3 | 5 | ×4 |
| B1 | Q76R | 3.7 | ×4 | 9.5 | ×4 | 5.9 | ×4 |
| QE/RG | Q76R, E360G | 2.4 | ×7 | 9.2 | ×4 | 2.2 | ×10 |
| T50K | 2.9 | ×6 | 5.3 | ×6 | 3.8 | ×6 | |
| C11 | N58K | 2.8 | ×6 | 7.9 | ×4 | 4.7 | ×5 |
| NE/KG | N58K, E360G | 2.2 | ×7 | 5.6 | ×6 | 2.7 | ×8 |
| . | . | UAA . | UAG . | UGA . | |||
|---|---|---|---|---|---|---|---|
| . | . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . | Readthrough efficiency (%) . | Fold WT . |
| D96E | 7.3 | ×2 | 10.7 | ×2 | 12.3 | ×2 | |
| A2 | E360G | 4.8 | ×3 | 11.9 | ×3 | 6.9 | ×3 |
| A8 | E104K | 3.6 | ×5 | 10.4 | ×3 | 3.9 | ×6 |
| EE/KG | E104K, E360G | 3.2 | ×5 | 12.9 | ×3 | 5 | ×4 |
| B1 | Q76R | 3.7 | ×4 | 9.5 | ×4 | 5.9 | ×4 |
| QE/RG | Q76R, E360G | 2.4 | ×7 | 9.2 | ×4 | 2.2 | ×10 |
| T50K | 2.9 | ×6 | 5.3 | ×6 | 3.8 | ×6 | |
| C11 | N58K | 2.8 | ×6 | 7.9 | ×4 | 4.7 | ×5 |
| NE/KG | N58K, E360G | 2.2 | ×7 | 5.6 | ×6 | 2.7 | ×8 |
Same legend as for Table 2. The name of the allele is indicated in the left column for the mutants identified during the screening, and for the double mutants.
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