| Rubber biosynthesis pathway genes | GenBank accession no. | EST frequency | Relative latex transcript level based on Pfaffl ratio | Mean CP±SD |
| REF | X56535 | 1150 | 426 | 9.10±0.80 |
| SRPP | AF051317 | 375 | 2932 | 5.51±0.28 |
| Cis-prenyl-transferase | AB061234 | 31 | 41 | 13.71±2.62 |
| Patatin-like inhibitor | AF113546 | 31 | 11 | 14.38±0.72 |
| HMG CoA synthase | AY534617 | 15 | 7 | 14.79±0.10 |
| HMG CoA reductase | X54659 | 10 | 4 | 16.21±1.12 |
| Rubber biosynthesis stimulator protein | AF516353 | 3 | 4 | 16.96±1.99 |
| GGPP synthase | AB055496 | 3 | 1 | 16.71±0.01 |
| DXPS | U27099 | 1 | 13 | 13.64±0.28 |
| Mevalonate kinase | AF429384 | 0 | 1 | 18.63±0.42 |
| Phosphomevalonate kinase | AF429385 | 0 | 17 | 13.17±0.03 |
| FPP synthase | Z49786 | 0 | 10 | 13.86±0.32 |
| IDP isomerase | BD301623 | 0 | 1 | 19.13±0.60 |
| 18S rRNA | AY496880 | NA | 10 000 | 4.14±0.45 |
| Rubber biosynthesis pathway genes | GenBank accession no. | EST frequency | Relative latex transcript level based on Pfaffl ratio | Mean CP±SD |
| REF | X56535 | 1150 | 426 | 9.10±0.80 |
| SRPP | AF051317 | 375 | 2932 | 5.51±0.28 |
| Cis-prenyl-transferase | AB061234 | 31 | 41 | 13.71±2.62 |
| Patatin-like inhibitor | AF113546 | 31 | 11 | 14.38±0.72 |
| HMG CoA synthase | AY534617 | 15 | 7 | 14.79±0.10 |
| HMG CoA reductase | X54659 | 10 | 4 | 16.21±1.12 |
| Rubber biosynthesis stimulator protein | AF516353 | 3 | 4 | 16.96±1.99 |
| GGPP synthase | AB055496 | 3 | 1 | 16.71±0.01 |
| DXPS | U27099 | 1 | 13 | 13.64±0.28 |
| Mevalonate kinase | AF429384 | 0 | 1 | 18.63±0.42 |
| Phosphomevalonate kinase | AF429385 | 0 | 17 | 13.17±0.03 |
| FPP synthase | Z49786 | 0 | 10 | 13.86±0.32 |
| IDP isomerase | BD301623 | 0 | 1 | 19.13±0.60 |
| 18S rRNA | AY496880 | NA | 10 000 | 4.14±0.45 |
Expression levels are based on EST frequency by BLASTN search and relative transcript level by QRT-PCR analysis of latex total RNA. BLASTN searches were performed using rubber biosynthesis-related cDNAs available for Hevea in all cases, except for DXPS where the Arabidopsis cDNA was used. Primer pairs for QRT-PCRs were designed for Hevea rubber biosynthesis-related cDNA targets including Hevea DXPS that was detected by the Arabidopsis DXPS. Transcript levels were calculated relative to Hevea 18S according to the Pfaffl mathematical model (2001) where CP is the cycle threshold. NA, not applicable; SD, standard deviation.
| Rubber biosynthesis pathway genes | GenBank accession no. | EST frequency | Relative latex transcript level based on Pfaffl ratio | Mean CP±SD |
| REF | X56535 | 1150 | 426 | 9.10±0.80 |
| SRPP | AF051317 | 375 | 2932 | 5.51±0.28 |
| Cis-prenyl-transferase | AB061234 | 31 | 41 | 13.71±2.62 |
| Patatin-like inhibitor | AF113546 | 31 | 11 | 14.38±0.72 |
| HMG CoA synthase | AY534617 | 15 | 7 | 14.79±0.10 |
| HMG CoA reductase | X54659 | 10 | 4 | 16.21±1.12 |
| Rubber biosynthesis stimulator protein | AF516353 | 3 | 4 | 16.96±1.99 |
| GGPP synthase | AB055496 | 3 | 1 | 16.71±0.01 |
| DXPS | U27099 | 1 | 13 | 13.64±0.28 |
| Mevalonate kinase | AF429384 | 0 | 1 | 18.63±0.42 |
| Phosphomevalonate kinase | AF429385 | 0 | 17 | 13.17±0.03 |
| FPP synthase | Z49786 | 0 | 10 | 13.86±0.32 |
| IDP isomerase | BD301623 | 0 | 1 | 19.13±0.60 |
| 18S rRNA | AY496880 | NA | 10 000 | 4.14±0.45 |
| Rubber biosynthesis pathway genes | GenBank accession no. | EST frequency | Relative latex transcript level based on Pfaffl ratio | Mean CP±SD |
| REF | X56535 | 1150 | 426 | 9.10±0.80 |
| SRPP | AF051317 | 375 | 2932 | 5.51±0.28 |
| Cis-prenyl-transferase | AB061234 | 31 | 41 | 13.71±2.62 |
| Patatin-like inhibitor | AF113546 | 31 | 11 | 14.38±0.72 |
| HMG CoA synthase | AY534617 | 15 | 7 | 14.79±0.10 |
| HMG CoA reductase | X54659 | 10 | 4 | 16.21±1.12 |
| Rubber biosynthesis stimulator protein | AF516353 | 3 | 4 | 16.96±1.99 |
| GGPP synthase | AB055496 | 3 | 1 | 16.71±0.01 |
| DXPS | U27099 | 1 | 13 | 13.64±0.28 |
| Mevalonate kinase | AF429384 | 0 | 1 | 18.63±0.42 |
| Phosphomevalonate kinase | AF429385 | 0 | 17 | 13.17±0.03 |
| FPP synthase | Z49786 | 0 | 10 | 13.86±0.32 |
| IDP isomerase | BD301623 | 0 | 1 | 19.13±0.60 |
| 18S rRNA | AY496880 | NA | 10 000 | 4.14±0.45 |
Expression levels are based on EST frequency by BLASTN search and relative transcript level by QRT-PCR analysis of latex total RNA. BLASTN searches were performed using rubber biosynthesis-related cDNAs available for Hevea in all cases, except for DXPS where the Arabidopsis cDNA was used. Primer pairs for QRT-PCRs were designed for Hevea rubber biosynthesis-related cDNA targets including Hevea DXPS that was detected by the Arabidopsis DXPS. Transcript levels were calculated relative to Hevea 18S according to the Pfaffl mathematical model (2001) where CP is the cycle threshold. NA, not applicable; SD, standard deviation.
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