Table 1.

General statistics of latex EST sequence analysis

No. of sequences(%)
Raw sequences10 193
Quality trimmed sequences (phred20)10 040
Clustering analysis:
    Clusters1127
    Contigs1321
    Consensus sequences1380
    Singletons2061
Unique transcripts (UTs)3441a(34.3)b
Redundant ESTs6599(65.7)b
Functional analysis:
    UTs with GO match902(26.2)c
        Molecular function736
        Biological process543
        Cellular component349
    UTs without GO match2539(73.8)c
No. of sequences(%)
Raw sequences10 193
Quality trimmed sequences (phred20)10 040
Clustering analysis:
    Clusters1127
    Contigs1321
    Consensus sequences1380
    Singletons2061
Unique transcripts (UTs)3441a(34.3)b
Redundant ESTs6599(65.7)b
Functional analysis:
    UTs with GO match902(26.2)c
        Molecular function736
        Biological process543
        Cellular component349
    UTs without GO match2539(73.8)c

EST sequences were assembled into clusters and contigs followed by generation of consensus sequences (StackPack version 2.2). UTs were classified according to the Gene Ontology (GO) functional categories: molecular function, biological process, and cellular component, where each included UTs classified in more than one category.

a

Number of unique transcripts is the total of consensus sequences and singletons.

b

Percentage calculated from a total of 10 040 ESTs.

c

Percentage calculated from a total of 3441 UTs.

Table 1.

General statistics of latex EST sequence analysis

No. of sequences(%)
Raw sequences10 193
Quality trimmed sequences (phred20)10 040
Clustering analysis:
    Clusters1127
    Contigs1321
    Consensus sequences1380
    Singletons2061
Unique transcripts (UTs)3441a(34.3)b
Redundant ESTs6599(65.7)b
Functional analysis:
    UTs with GO match902(26.2)c
        Molecular function736
        Biological process543
        Cellular component349
    UTs without GO match2539(73.8)c
No. of sequences(%)
Raw sequences10 193
Quality trimmed sequences (phred20)10 040
Clustering analysis:
    Clusters1127
    Contigs1321
    Consensus sequences1380
    Singletons2061
Unique transcripts (UTs)3441a(34.3)b
Redundant ESTs6599(65.7)b
Functional analysis:
    UTs with GO match902(26.2)c
        Molecular function736
        Biological process543
        Cellular component349
    UTs without GO match2539(73.8)c

EST sequences were assembled into clusters and contigs followed by generation of consensus sequences (StackPack version 2.2). UTs were classified according to the Gene Ontology (GO) functional categories: molecular function, biological process, and cellular component, where each included UTs classified in more than one category.

a

Number of unique transcripts is the total of consensus sequences and singletons.

b

Percentage calculated from a total of 10 040 ESTs.

c

Percentage calculated from a total of 3441 UTs.

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