Open in new tab LexA box sequences...

Organism	LexA box consensus 5′-3′	Method of determination	References
Gram-negatives
Beta- and Gammaproteobacteria	CTGTN₈ACAG	In silico	Erill et al. (2003)
E. coli	TACTGTATATATATACAGTA	In vitro, in vivo	Fernandez De Henestrosa et al. (2000) and Wade et al. (2005)
Alphaproteobacteria
Rhodobacter sphaeroides	GTTCN₇GTTC	In vitro	Fernandez de Henestrosa et al. (1998)
Rhizobium and Agrobacterium	GAACN₇GTAC	In vitro, in vivo	Tapias & Barbe (1998)
Cyanobacteria	RGTACN₃DGTWCB	In vitro	Mazon et al. (2004a,b)
Spirocheate
Leptospira interrogans	TTTGN₅CAAA	In vitro	Fonseca et al. (2013)
Acidobacteria
Acidobacterium capsulatum	GTTCN₇GTTC	In vitro, in vivo	Mazon et al. (2006)
Gram-positives	GAACN₄GTTC
Firmicutes
Bacillus subtilis	CGAACRNRYGTTYC	In vitro, in silico	Winterling et al. (1998)
Chloroflexi
Dehalococcoides ethenogenes	GAACN₄GTTC	In vitro	Fernandez de Henestrosa et al. (2002)
Actinobacteria
Mycobacterium	TCGAACN₄GTTCGA	In vivo	Davis et al. (2002)
Deinococcus
Deinococcus radiodurans	CGAACRNRYGTTCG	Algorithm	Khan et al. (2008)
Integrons	ACTGTW₈ACAGT	In silico	Cambray et al. (2011)

Organism

LexA box consensus 5′-3′

Method of determination

References

Gram-negatives

Beta- and Gammaproteobacteria

CTGTN₈ACAG

In silico

Erill et al. (2003)

E. coli

TACTGTATATATATACAGTA

In vitro, in vivo

Fernandez De Henestrosa et al. (2000) and Wade et al. (2005)

Alphaproteobacteria

Rhodobacter sphaeroides

GTTCN₇GTTC

In vitro

Fernandez de Henestrosa et al. (1998)

Rhizobium and Agrobacterium

GAACN₇GTAC

In vitro, in vivo

Tapias & Barbe (1998)

Cyanobacteria

RGTACN₃DGTWCB

In vitro

Mazon et al. (2004a,b)

Spirocheate

Leptospira interrogans

TTTGN₅CAAA

In vitro

Fonseca et al. (2013)

Acidobacteria

Acidobacterium capsulatum

GTTCN₇GTTC

In vitro, in vivo

Mazon et al. (2006)

Gram-positives

GAACN₄GTTC

Firmicutes

Bacillus subtilis

CGAACRNRYGTTYC

In vitro, in silico

Winterling et al. (1998)

Chloroflexi

Dehalococcoides ethenogenes

GAACN₄GTTC

In vitro

Fernandez de Henestrosa et al. (2002)

Actinobacteria

Mycobacterium

TCGAACN₄GTTCGA

In vivo

Davis et al. (2002)

Deinococcus

Deinococcus radiodurans

CGAACRNRYGTTCG

Algorithm

Khan et al. (2008)

Integrons

ACTGTW₈ACAGT

In silico

Cambray et al. (2011)

Organism	LexA box consensus 5′-3′	Method of determination	References
Gram-negatives
Beta- and Gammaproteobacteria	CTGTN₈ACAG	In silico	Erill et al. (2003)
E. coli	TACTGTATATATATACAGTA	In vitro, in vivo	Fernandez De Henestrosa et al. (2000) and Wade et al. (2005)
Alphaproteobacteria
Rhodobacter sphaeroides	GTTCN₇GTTC	In vitro	Fernandez de Henestrosa et al. (1998)
Rhizobium and Agrobacterium	GAACN₇GTAC	In vitro, in vivo	Tapias & Barbe (1998)
Cyanobacteria	RGTACN₃DGTWCB	In vitro	Mazon et al. (2004a,b)
Spirocheate
Leptospira interrogans	TTTGN₅CAAA	In vitro	Fonseca et al. (2013)
Acidobacteria
Acidobacterium capsulatum	GTTCN₇GTTC	In vitro, in vivo	Mazon et al. (2006)
Gram-positives	GAACN₄GTTC
Firmicutes
Bacillus subtilis	CGAACRNRYGTTYC	In vitro, in silico	Winterling et al. (1998)
Chloroflexi
Dehalococcoides ethenogenes	GAACN₄GTTC	In vitro	Fernandez de Henestrosa et al. (2002)
Actinobacteria
Mycobacterium	TCGAACN₄GTTCGA	In vivo	Davis et al. (2002)
Deinococcus
Deinococcus radiodurans	CGAACRNRYGTTCG	Algorithm	Khan et al. (2008)
Integrons	ACTGTW₈ACAGT	In silico	Cambray et al. (2011)

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LexA box sequences identified across different bacterial species

Organism	LexA box consensus 5′-3′	Method of determination	References
Gram-negatives
Beta- and Gammaproteobacteria	CTGTN₈ACAG	In silico	Erill et al. (2003)
E. coli	TACTGTATATATATACAGTA	In vitro, in vivo	Fernandez De Henestrosa et al. (2000) and Wade et al. (2005)
Alphaproteobacteria
Rhodobacter sphaeroides	GTTCN₇GTTC	In vitro	Fernandez de Henestrosa et al. (1998)
Rhizobium and Agrobacterium	GAACN₇GTAC	In vitro, in vivo	Tapias & Barbe (1998)
Cyanobacteria	RGTACN₃DGTWCB	In vitro	Mazon et al. (2004a,b)
Spirocheate
Leptospira interrogans	TTTGN₅CAAA	In vitro	Fonseca et al. (2013)
Acidobacteria
Acidobacterium capsulatum	GTTCN₇GTTC	In vitro, in vivo	Mazon et al. (2006)
Gram-positives	GAACN₄GTTC
Firmicutes
Bacillus subtilis	CGAACRNRYGTTYC	In vitro, in silico	Winterling et al. (1998)
Chloroflexi
Dehalococcoides ethenogenes	GAACN₄GTTC	In vitro	Fernandez de Henestrosa et al. (2002)
Actinobacteria
Mycobacterium	TCGAACN₄GTTCGA	In vivo	Davis et al. (2002)
Deinococcus
Deinococcus radiodurans	CGAACRNRYGTTCG	Algorithm	Khan et al. (2008)
Integrons	ACTGTW₈ACAGT	In silico	Cambray et al. (2011)

Organism	LexA box consensus 5′-3′	Method of determination	References
Gram-negatives
Beta- and Gammaproteobacteria	CTGTN₈ACAG	In silico	Erill et al. (2003)
E. coli	TACTGTATATATATACAGTA	In vitro, in vivo	Fernandez De Henestrosa et al. (2000) and Wade et al. (2005)
Alphaproteobacteria
Rhodobacter sphaeroides	GTTCN₇GTTC	In vitro	Fernandez de Henestrosa et al. (1998)
Rhizobium and Agrobacterium	GAACN₇GTAC	In vitro, in vivo	Tapias & Barbe (1998)
Cyanobacteria	RGTACN₃DGTWCB	In vitro	Mazon et al. (2004a,b)
Spirocheate
Leptospira interrogans	TTTGN₅CAAA	In vitro	Fonseca et al. (2013)
Acidobacteria
Acidobacterium capsulatum	GTTCN₇GTTC	In vitro, in vivo	Mazon et al. (2006)
Gram-positives	GAACN₄GTTC
Firmicutes
Bacillus subtilis	CGAACRNRYGTTYC	In vitro, in silico	Winterling et al. (1998)
Chloroflexi
Dehalococcoides ethenogenes	GAACN₄GTTC	In vitro	Fernandez de Henestrosa et al. (2002)
Actinobacteria
Mycobacterium	TCGAACN₄GTTCGA	In vivo	Davis et al. (2002)
Deinococcus
Deinococcus radiodurans	CGAACRNRYGTTCG	Algorithm	Khan et al. (2008)
Integrons	ACTGTW₈ACAGT	In silico	Cambray et al. (2011)

Even though the two LexA paralogues do not appear to play a role in the radioresistance of D. radiodurans (Narumi et al., 2001; Jolivet et al., 2006), several studies point to a role of RecA recruitment at DNA lesions and intracellular LexA levels (Satoh et al., 2006, 2012). Algorithm-based studies identified a conserved sequence related to the Bacillus subtilis LexA box upstream of the known SOS-regulated genes (Khan et al., 2008). Y: C or T; R: A or G; W: A or T; D: not C; B: not A; N: any base.

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