4

Antagonistic and plant growth promoting mechanisms of Pseudomonas putida isolates in vitro

GroupaStrainOriginAntagonistic activity towardcLytic enzymesdMetabolites
HabitatStagebV.d.R.s.CellulasesGlucanasesProteasesDAPGeIAAf
G12Re4–1Endorhizaf++++++
2Re4–4Endorhizaf++++++
G22Re4–9Endorhizaf+++++
2Re4-10Endorhizaf+++++
2Re4-11Endorhizaf++++++
2Re4–6Endorhizaf++++++
2Re4–7Endorhizaf+++++
G35Re4-27Endorhizaf++++
5R2–4Rhizospheref+++++++
5Re2-21Endorhizaf+++++
5Re4–2Endorhizaf+++++
5R2-27Rhizospheref+++++++
5Re4-19Endorhizaf++++++
5Re2-27Endorhizaf++++++
5Re4–3Endorhizaf+++++
5Re1-25Endorhizaf+++++++
6Re2–8Endorhizas++++
6Re2-20Endorhizas+++++
6Re3–9Endorhizas+++++
6Re4-27Endorhizas+++++
G46R5-19Rhizospheres+++++
6R5–4Rhizospheres++++++
6R5–2Rhizospheres+++++
6R5-24Rhizospheres++++++++
G56Re2-14Endorhizas+++++
6Re2-16Endorhizas+++++
6Re2–5Endorhizas+++++
G66Re2-22Endorhizas+++++++
G74R2-10Rhizospherey++
G83R1-19Rhizospheres++++++++
3R2-12Rhizospheres+++++++
G92Re4-27Endorhizaf++++
2Re4-32Endorhizaf++++++
2Re4-30Endorhizaf++++
2Re4-23Endorhizaf++++++
2Re4-24Endorhizaf+++++
2Re4-26Endorhizaf+++++
G102Re4-28Endorhizaf+++++
G112Re4-31Endorhizaf+++++
2Re4-33Endorhizaf++++
2Re4-35Endorhizaf+++++
2Re4-36Endorhizaf++++
G122Re4-16Endorhizaf++++
2Re4-18Endorhizaf++++
2Re4-19Endorhizaf++++
G136Re2–4Endorhizas++++++
G146R2–2Rhizospheres+++++
G153R4-17Rhizospheres+++
G163Re4–3Rhizospheres+++
G173Re4-21Endorhizas+++++++++
G185P2-29Phyllospheref++++
5P2-15Phyllospheref++++
5P2-24Phyllospheref+++
5P2-20Phyllospheref++++
5P2-21Phyllospheref++++
G195P2-16Phyllospheref+++
5P2-22Phyllospheref+++
G205P2-12Phyllospheref+++
G215P2-28Phyllospheref+++++
G225Re5-12Endorhizaf+++
G235Re2–6Endorhizaf++++++
5Re4-23Endorhizaf+++++++
5Re2-10Endorhizaf+++++++
5Re2-17Endorhizaf+++++++
5Re2-28Endorhizaf++++++++
G245Re4-16Endorhizaf+++
G256Re4–2Endorhizas+++++++++
G262Re2-31Endorhizaf++++++
GroupaStrainOriginAntagonistic activity towardcLytic enzymesdMetabolites
HabitatStagebV.d.R.s.CellulasesGlucanasesProteasesDAPGeIAAf
G12Re4–1Endorhizaf++++++
2Re4–4Endorhizaf++++++
G22Re4–9Endorhizaf+++++
2Re4-10Endorhizaf+++++
2Re4-11Endorhizaf++++++
2Re4–6Endorhizaf++++++
2Re4–7Endorhizaf+++++
G35Re4-27Endorhizaf++++
5R2–4Rhizospheref+++++++
5Re2-21Endorhizaf+++++
5Re4–2Endorhizaf+++++
5R2-27Rhizospheref+++++++
5Re4-19Endorhizaf++++++
5Re2-27Endorhizaf++++++
5Re4–3Endorhizaf+++++
5Re1-25Endorhizaf+++++++
6Re2–8Endorhizas++++
6Re2-20Endorhizas+++++
6Re3–9Endorhizas+++++
6Re4-27Endorhizas+++++
G46R5-19Rhizospheres+++++
6R5–4Rhizospheres++++++
6R5–2Rhizospheres+++++
6R5-24Rhizospheres++++++++
G56Re2-14Endorhizas+++++
6Re2-16Endorhizas+++++
6Re2–5Endorhizas+++++
G66Re2-22Endorhizas+++++++
G74R2-10Rhizospherey++
G83R1-19Rhizospheres++++++++
3R2-12Rhizospheres+++++++
G92Re4-27Endorhizaf++++
2Re4-32Endorhizaf++++++
2Re4-30Endorhizaf++++
2Re4-23Endorhizaf++++++
2Re4-24Endorhizaf+++++
2Re4-26Endorhizaf+++++
G102Re4-28Endorhizaf+++++
G112Re4-31Endorhizaf+++++
2Re4-33Endorhizaf++++
2Re4-35Endorhizaf+++++
2Re4-36Endorhizaf++++
G122Re4-16Endorhizaf++++
2Re4-18Endorhizaf++++
2Re4-19Endorhizaf++++
G136Re2–4Endorhizas++++++
G146R2–2Rhizospheres+++++
G153R4-17Rhizospheres+++
G163Re4–3Rhizospheres+++
G173Re4-21Endorhizas+++++++++
G185P2-29Phyllospheref++++
5P2-15Phyllospheref++++
5P2-24Phyllospheref+++
5P2-20Phyllospheref++++
5P2-21Phyllospheref++++
G195P2-16Phyllospheref+++
5P2-22Phyllospheref+++
G205P2-12Phyllospheref+++
G215P2-28Phyllospheref+++++
G225Re5-12Endorhizaf+++
G235Re2–6Endorhizaf++++++
5Re4-23Endorhizaf+++++++
5Re2-10Endorhizaf+++++++
5Re2-17Endorhizaf+++++++
5Re2-28Endorhizaf++++++++
G245Re4-16Endorhizaf+++
G256Re4–2Endorhizas+++++++++
G262Re2-31Endorhizaf++++++

aGrouping according to BOX-PCR fingerprinting and clustering at a similarity level of 90%.

by, Young plants; f, flowering plants; s, senescent plants.

cAntagonism toward V.d.=Verticillium dahliae, R.s.=Rhizoctonia solani, dual culture assay: + represents 0–5 mm wide zone, ++ represents 5–10 mm wide zone, +++ represents >10 mm wide zone of inhibition.

d+ represents production on plate assay.

eDetection of the phl gene by PCR.

fIAA = indole-3-acetic acid production in vitro.

4

Antagonistic and plant growth promoting mechanisms of Pseudomonas putida isolates in vitro

GroupaStrainOriginAntagonistic activity towardcLytic enzymesdMetabolites
HabitatStagebV.d.R.s.CellulasesGlucanasesProteasesDAPGeIAAf
G12Re4–1Endorhizaf++++++
2Re4–4Endorhizaf++++++
G22Re4–9Endorhizaf+++++
2Re4-10Endorhizaf+++++
2Re4-11Endorhizaf++++++
2Re4–6Endorhizaf++++++
2Re4–7Endorhizaf+++++
G35Re4-27Endorhizaf++++
5R2–4Rhizospheref+++++++
5Re2-21Endorhizaf+++++
5Re4–2Endorhizaf+++++
5R2-27Rhizospheref+++++++
5Re4-19Endorhizaf++++++
5Re2-27Endorhizaf++++++
5Re4–3Endorhizaf+++++
5Re1-25Endorhizaf+++++++
6Re2–8Endorhizas++++
6Re2-20Endorhizas+++++
6Re3–9Endorhizas+++++
6Re4-27Endorhizas+++++
G46R5-19Rhizospheres+++++
6R5–4Rhizospheres++++++
6R5–2Rhizospheres+++++
6R5-24Rhizospheres++++++++
G56Re2-14Endorhizas+++++
6Re2-16Endorhizas+++++
6Re2–5Endorhizas+++++
G66Re2-22Endorhizas+++++++
G74R2-10Rhizospherey++
G83R1-19Rhizospheres++++++++
3R2-12Rhizospheres+++++++
G92Re4-27Endorhizaf++++
2Re4-32Endorhizaf++++++
2Re4-30Endorhizaf++++
2Re4-23Endorhizaf++++++
2Re4-24Endorhizaf+++++
2Re4-26Endorhizaf+++++
G102Re4-28Endorhizaf+++++
G112Re4-31Endorhizaf+++++
2Re4-33Endorhizaf++++
2Re4-35Endorhizaf+++++
2Re4-36Endorhizaf++++
G122Re4-16Endorhizaf++++
2Re4-18Endorhizaf++++
2Re4-19Endorhizaf++++
G136Re2–4Endorhizas++++++
G146R2–2Rhizospheres+++++
G153R4-17Rhizospheres+++
G163Re4–3Rhizospheres+++
G173Re4-21Endorhizas+++++++++
G185P2-29Phyllospheref++++
5P2-15Phyllospheref++++
5P2-24Phyllospheref+++
5P2-20Phyllospheref++++
5P2-21Phyllospheref++++
G195P2-16Phyllospheref+++
5P2-22Phyllospheref+++
G205P2-12Phyllospheref+++
G215P2-28Phyllospheref+++++
G225Re5-12Endorhizaf+++
G235Re2–6Endorhizaf++++++
5Re4-23Endorhizaf+++++++
5Re2-10Endorhizaf+++++++
5Re2-17Endorhizaf+++++++
5Re2-28Endorhizaf++++++++
G245Re4-16Endorhizaf+++
G256Re4–2Endorhizas+++++++++
G262Re2-31Endorhizaf++++++
GroupaStrainOriginAntagonistic activity towardcLytic enzymesdMetabolites
HabitatStagebV.d.R.s.CellulasesGlucanasesProteasesDAPGeIAAf
G12Re4–1Endorhizaf++++++
2Re4–4Endorhizaf++++++
G22Re4–9Endorhizaf+++++
2Re4-10Endorhizaf+++++
2Re4-11Endorhizaf++++++
2Re4–6Endorhizaf++++++
2Re4–7Endorhizaf+++++
G35Re4-27Endorhizaf++++
5R2–4Rhizospheref+++++++
5Re2-21Endorhizaf+++++
5Re4–2Endorhizaf+++++
5R2-27Rhizospheref+++++++
5Re4-19Endorhizaf++++++
5Re2-27Endorhizaf++++++
5Re4–3Endorhizaf+++++
5Re1-25Endorhizaf+++++++
6Re2–8Endorhizas++++
6Re2-20Endorhizas+++++
6Re3–9Endorhizas+++++
6Re4-27Endorhizas+++++
G46R5-19Rhizospheres+++++
6R5–4Rhizospheres++++++
6R5–2Rhizospheres+++++
6R5-24Rhizospheres++++++++
G56Re2-14Endorhizas+++++
6Re2-16Endorhizas+++++
6Re2–5Endorhizas+++++
G66Re2-22Endorhizas+++++++
G74R2-10Rhizospherey++
G83R1-19Rhizospheres++++++++
3R2-12Rhizospheres+++++++
G92Re4-27Endorhizaf++++
2Re4-32Endorhizaf++++++
2Re4-30Endorhizaf++++
2Re4-23Endorhizaf++++++
2Re4-24Endorhizaf+++++
2Re4-26Endorhizaf+++++
G102Re4-28Endorhizaf+++++
G112Re4-31Endorhizaf+++++
2Re4-33Endorhizaf++++
2Re4-35Endorhizaf+++++
2Re4-36Endorhizaf++++
G122Re4-16Endorhizaf++++
2Re4-18Endorhizaf++++
2Re4-19Endorhizaf++++
G136Re2–4Endorhizas++++++
G146R2–2Rhizospheres+++++
G153R4-17Rhizospheres+++
G163Re4–3Rhizospheres+++
G173Re4-21Endorhizas+++++++++
G185P2-29Phyllospheref++++
5P2-15Phyllospheref++++
5P2-24Phyllospheref+++
5P2-20Phyllospheref++++
5P2-21Phyllospheref++++
G195P2-16Phyllospheref+++
5P2-22Phyllospheref+++
G205P2-12Phyllospheref+++
G215P2-28Phyllospheref+++++
G225Re5-12Endorhizaf+++
G235Re2–6Endorhizaf++++++
5Re4-23Endorhizaf+++++++
5Re2-10Endorhizaf+++++++
5Re2-17Endorhizaf+++++++
5Re2-28Endorhizaf++++++++
G245Re4-16Endorhizaf+++
G256Re4–2Endorhizas+++++++++
G262Re2-31Endorhizaf++++++

aGrouping according to BOX-PCR fingerprinting and clustering at a similarity level of 90%.

by, Young plants; f, flowering plants; s, senescent plants.

cAntagonism toward V.d.=Verticillium dahliae, R.s.=Rhizoctonia solani, dual culture assay: + represents 0–5 mm wide zone, ++ represents 5–10 mm wide zone, +++ represents >10 mm wide zone of inhibition.

d+ represents production on plate assay.

eDetection of the phl gene by PCR.

fIAA = indole-3-acetic acid production in vitro.

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