Sequence of PCR primers designed to amplify specific gene fragments of the parathion hydrolase-encoding gene of FB or other parathion-degrading bacteria present in the mixed continuous cultures derived from surface watera
| Name | Nucleotide sequence | Size of PCR product (bp) | ||
| with F196 | with F225 | with F450 | ||
| F196 | 5′-CGCGTCCTATCACAATCTC-3′ | X | X | X |
| F225 | 5′-TTTCACACTGACTCACGAGG-3′ | X | X | X |
| F450 | 5′-CGCCACTTTCGATGCGAT-3′ | X | X | X |
| R468 | 5′-TCGCATCGAAAGTGGCGG-3′ | 290 | 262 | X |
| R603 | 5′-ACACTAACTCCTGAAAGGGG-3′ | 426b | 398 | 153 |
| R757 | 5′-TCAGTATCATCGCTGTGACC-3′ | 580 | 552 | 308 |
| R840 | 5′-CTTCTAGACCAATCGCACTG-3′ | 663b | 635 | 391 |
| Name | Nucleotide sequence | Size of PCR product (bp) | ||
| with F196 | with F225 | with F450 | ||
| F196 | 5′-CGCGTCCTATCACAATCTC-3′ | X | X | X |
| F225 | 5′-TTTCACACTGACTCACGAGG-3′ | X | X | X |
| F450 | 5′-CGCCACTTTCGATGCGAT-3′ | X | X | X |
| R468 | 5′-TCGCATCGAAAGTGGCGG-3′ | 290 | 262 | X |
| R603 | 5′-ACACTAACTCCTGAAAGGGG-3′ | 426b | 398 | 153 |
| R757 | 5′-TCAGTATCATCGCTGTGACC-3′ | 580 | 552 | 308 |
| R840 | 5′-CTTCTAGACCAATCGCACTG-3′ | 663b | 635 | 391 |
Names consist of the letter F (forward) or R (reverse) followed by the number of base pairs in the gene sequence between the first base of the initiation codon and the nucleotide to which the 5′ nucleotide of the primer is complementary. The right three columns list the expected fragment size of the reverse primers in combination with each of the three forward primers. The combination of F450 and R468 was not investigated since these two primers are complementary to the same region of the gene.
Fragments used to screen DNA for parathion hydrolase gene.
Sequence of PCR primers designed to amplify specific gene fragments of the parathion hydrolase-encoding gene of FB or other parathion-degrading bacteria present in the mixed continuous cultures derived from surface watera
| Name | Nucleotide sequence | Size of PCR product (bp) | ||
| with F196 | with F225 | with F450 | ||
| F196 | 5′-CGCGTCCTATCACAATCTC-3′ | X | X | X |
| F225 | 5′-TTTCACACTGACTCACGAGG-3′ | X | X | X |
| F450 | 5′-CGCCACTTTCGATGCGAT-3′ | X | X | X |
| R468 | 5′-TCGCATCGAAAGTGGCGG-3′ | 290 | 262 | X |
| R603 | 5′-ACACTAACTCCTGAAAGGGG-3′ | 426b | 398 | 153 |
| R757 | 5′-TCAGTATCATCGCTGTGACC-3′ | 580 | 552 | 308 |
| R840 | 5′-CTTCTAGACCAATCGCACTG-3′ | 663b | 635 | 391 |
| Name | Nucleotide sequence | Size of PCR product (bp) | ||
| with F196 | with F225 | with F450 | ||
| F196 | 5′-CGCGTCCTATCACAATCTC-3′ | X | X | X |
| F225 | 5′-TTTCACACTGACTCACGAGG-3′ | X | X | X |
| F450 | 5′-CGCCACTTTCGATGCGAT-3′ | X | X | X |
| R468 | 5′-TCGCATCGAAAGTGGCGG-3′ | 290 | 262 | X |
| R603 | 5′-ACACTAACTCCTGAAAGGGG-3′ | 426b | 398 | 153 |
| R757 | 5′-TCAGTATCATCGCTGTGACC-3′ | 580 | 552 | 308 |
| R840 | 5′-CTTCTAGACCAATCGCACTG-3′ | 663b | 635 | 391 |
Names consist of the letter F (forward) or R (reverse) followed by the number of base pairs in the gene sequence between the first base of the initiation codon and the nucleotide to which the 5′ nucleotide of the primer is complementary. The right three columns list the expected fragment size of the reverse primers in combination with each of the three forward primers. The combination of F450 and R468 was not investigated since these two primers are complementary to the same region of the gene.
Fragments used to screen DNA for parathion hydrolase gene.
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