Table 1. Open in new tab Most abundant...

Contig	No. of clones	Source library	Putative function	Nearest match	e-value
Developmental libraries
Contig 175	102	S0S1	Metallothionein-like protein	AY833008	7E-24
Contig 172	49	S0S1	Large subunit rRNA	DQ008809	1E-73
Contig 180	40	S2S4	Metallothionein type II	AF039003	9E-27
Contig 19	30	S0S1	Metallothionein type II	AF039003	9E-27
Contig 239	19	S0S1	Metallothionein type II	AF147786	4E-23
Contig 252	19	S2S4	Metallothionein type II	AF039003	2E-28
Contig 35	19	S2S4	Metallothionein-like protein	AF279655	3E-30
Contig 158	17	SSH0-2	Putative cell wall P8 protein	AC146631	0.0009
Contig 203	16	S0S1	Cytochrome b6/f complex subunit VIII	BAF64867	0.001
Contig 198	15	S0S1	Ubiquitin carrier-like protein	DQ294271	1E-101
Contig 150	13	SSH0-2	GSDL-motif lipase	AY491975	6E-62
Contig 242	13	S2S4	Metallothionein type I	AF039002	1E-22
Contig 214	11	S0S1	40S ribosomal protein S17 (RPS17D)	AT5G04800	3E-64
Contig 128	10	SSH2-45	Dihydroflavonol-4-reductase	AY374471	1E-54
Contig 221	10	S2S4	Large subunit rRNA gene	DQ008808	0
Contig 45	10	SSH2-0	Aldehyde decarbonylase (epicuticular wax associated)	ATHCER1	2E-56
Stress libraries
Contig 175	48	COLD	Metallothionein-like protein	AY833008	7E-24
Contig 172	22	COLD	Large subunit rRNA	DQ008809	1E-73
Contig 19	15	COLD	Metallothionein type II	AF039003	9E-27
Contig 242	12	COLD	Metallothionein type I	AF039002	1E-22
Contig 263	12	COLD	Arabidopsis unknown protein/CXE carboxylesterase	AT1G47480	0
Contig 180	9	COLD	Metallothionein type II	AF039003	9E-27
Contig 186	5	COLD	No significant BLAST match
Contig 187	4	COLD	Elongation factor-1 alpha 2
Contig 239	4	COLD	Metallothionein-like protein (ML2)	AF147786	4E-23
Contig 252	4	COLD	Metallothionein type II	AF039003	2E-28
Contig 154	3	COLD	Ubiquitin precursor-like	EU249995	E-116
Contig 193	3	COLD	Glutathione transferase	AJ441055	4E-72
Contig 35	3	COLD	Metallothionein-like protein	AF279655	3E-30

Contig

No. of clones

Source library

Putative function

Nearest match

e-value

Developmental libraries

Contig 175

102

S0S1

Metallothionein-like protein

AY833008

7E-24

Contig 172

S0S1

Large subunit rRNA

DQ008809

1E-73

Contig 180

S2S4

Metallothionein type II

AF039003

9E-27

Contig 19

S0S1

Metallothionein type II

AF039003

9E-27

Contig 239

S0S1

Metallothionein type II

AF147786

4E-23

Contig 252

S2S4

Metallothionein type II

AF039003

2E-28

Contig 35

S2S4

Metallothionein-like protein

AF279655

3E-30

Contig 158

SSH0-2

Putative cell wall P8 protein

AC146631

0.0009

Contig 203

S0S1

Cytochrome b6/f complex subunit VIII

BAF64867

0.001

Contig 198

S0S1

Ubiquitin carrier-like protein

DQ294271

1E-101

Contig 150

SSH0-2

GSDL-motif lipase

AY491975

6E-62

Contig 242

S2S4

Metallothionein type I

AF039002

1E-22

Contig 214

S0S1

40S ribosomal protein S17 (RPS17D)

AT5G04800

3E-64

Contig 128

SSH2-45

Dihydroflavonol-4-reductase

AY374471

1E-54

Contig 221

S2S4

Large subunit rRNA gene

DQ008808

Contig 45

SSH2-0

Aldehyde decarbonylase (epicuticular wax associated)

ATHCER1

2E-56

Stress libraries

Contig 175

COLD

Metallothionein-like protein

AY833008

7E-24

Contig 172

COLD

Large subunit rRNA

DQ008809

1E-73

Contig 19

COLD

Metallothionein type II

AF039003

9E-27

Contig 242

COLD

Metallothionein type I

AF039002

1E-22

Contig 263

COLD

Arabidopsis unknown protein/CXE carboxylesterase

AT1G47480

Contig 180

COLD

Metallothionein type II

AF039003

9E-27

Contig 186

COLD

No significant BLAST match

Contig 187

COLD

Elongation factor-1 alpha 2

Contig 239

COLD

Metallothionein-like protein (ML2)

AF147786

4E-23

Contig 252

COLD

Metallothionein type II

AF039003

2E-28

Contig 154

COLD

Ubiquitin precursor-like

EU249995

E-116

Contig 193

COLD

Glutathione transferase

AJ441055

4E-72

Contig 35

COLD

Metallothionein-like protein

AF279655

3E-30

Contig	No. of clones	Source library	Putative function	Nearest match	e-value
Developmental libraries
Contig 175	102	S0S1	Metallothionein-like protein	AY833008	7E-24
Contig 172	49	S0S1	Large subunit rRNA	DQ008809	1E-73
Contig 180	40	S2S4	Metallothionein type II	AF039003	9E-27
Contig 19	30	S0S1	Metallothionein type II	AF039003	9E-27
Contig 239	19	S0S1	Metallothionein type II	AF147786	4E-23
Contig 252	19	S2S4	Metallothionein type II	AF039003	2E-28
Contig 35	19	S2S4	Metallothionein-like protein	AF279655	3E-30
Contig 158	17	SSH0-2	Putative cell wall P8 protein	AC146631	0.0009
Contig 203	16	S0S1	Cytochrome b6/f complex subunit VIII	BAF64867	0.001
Contig 198	15	S0S1	Ubiquitin carrier-like protein	DQ294271	1E-101
Contig 150	13	SSH0-2	GSDL-motif lipase	AY491975	6E-62
Contig 242	13	S2S4	Metallothionein type I	AF039002	1E-22
Contig 214	11	S0S1	40S ribosomal protein S17 (RPS17D)	AT5G04800	3E-64
Contig 128	10	SSH2-45	Dihydroflavonol-4-reductase	AY374471	1E-54
Contig 221	10	S2S4	Large subunit rRNA gene	DQ008808	0
Contig 45	10	SSH2-0	Aldehyde decarbonylase (epicuticular wax associated)	ATHCER1	2E-56
Stress libraries
Contig 175	48	COLD	Metallothionein-like protein	AY833008	7E-24
Contig 172	22	COLD	Large subunit rRNA	DQ008809	1E-73
Contig 19	15	COLD	Metallothionein type II	AF039003	9E-27
Contig 242	12	COLD	Metallothionein type I	AF039002	1E-22
Contig 263	12	COLD	Arabidopsis unknown protein/CXE carboxylesterase	AT1G47480	0
Contig 180	9	COLD	Metallothionein type II	AF039003	9E-27
Contig 186	5	COLD	No significant BLAST match
Contig 187	4	COLD	Elongation factor-1 alpha 2
Contig 239	4	COLD	Metallothionein-like protein (ML2)	AF147786	4E-23
Contig 252	4	COLD	Metallothionein type II	AF039003	2E-28
Contig 154	3	COLD	Ubiquitin precursor-like	EU249995	E-116
Contig 193	3	COLD	Glutathione transferase	AJ441055	4E-72
Contig 35	3	COLD	Metallothionein-like protein	AF279655	3E-30

Table 1.

Open in new tab

Most abundant classes of ESTs from developmental senescence and stress-treated libraries

Contig	No. of clones	Source library	Putative function	Nearest match	e-value
Developmental libraries
Contig 175	102	S0S1	Metallothionein-like protein	AY833008	7E-24
Contig 172	49	S0S1	Large subunit rRNA	DQ008809	1E-73
Contig 180	40	S2S4	Metallothionein type II	AF039003	9E-27
Contig 19	30	S0S1	Metallothionein type II	AF039003	9E-27
Contig 239	19	S0S1	Metallothionein type II	AF147786	4E-23
Contig 252	19	S2S4	Metallothionein type II	AF039003	2E-28
Contig 35	19	S2S4	Metallothionein-like protein	AF279655	3E-30
Contig 158	17	SSH0-2	Putative cell wall P8 protein	AC146631	0.0009
Contig 203	16	S0S1	Cytochrome b6/f complex subunit VIII	BAF64867	0.001
Contig 198	15	S0S1	Ubiquitin carrier-like protein	DQ294271	1E-101
Contig 150	13	SSH0-2	GSDL-motif lipase	AY491975	6E-62
Contig 242	13	S2S4	Metallothionein type I	AF039002	1E-22
Contig 214	11	S0S1	40S ribosomal protein S17 (RPS17D)	AT5G04800	3E-64
Contig 128	10	SSH2-45	Dihydroflavonol-4-reductase	AY374471	1E-54
Contig 221	10	S2S4	Large subunit rRNA gene	DQ008808	0
Contig 45	10	SSH2-0	Aldehyde decarbonylase (epicuticular wax associated)	ATHCER1	2E-56
Stress libraries
Contig 175	48	COLD	Metallothionein-like protein	AY833008	7E-24
Contig 172	22	COLD	Large subunit rRNA	DQ008809	1E-73
Contig 19	15	COLD	Metallothionein type II	AF039003	9E-27
Contig 242	12	COLD	Metallothionein type I	AF039002	1E-22
Contig 263	12	COLD	Arabidopsis unknown protein/CXE carboxylesterase	AT1G47480	0
Contig 180	9	COLD	Metallothionein type II	AF039003	9E-27
Contig 186	5	COLD	No significant BLAST match
Contig 187	4	COLD	Elongation factor-1 alpha 2
Contig 239	4	COLD	Metallothionein-like protein (ML2)	AF147786	4E-23
Contig 252	4	COLD	Metallothionein type II	AF039003	2E-28
Contig 154	3	COLD	Ubiquitin precursor-like	EU249995	E-116
Contig 193	3	COLD	Glutathione transferase	AJ441055	4E-72
Contig 35	3	COLD	Metallothionein-like protein	AF279655	3E-30

Contig	No. of clones	Source library	Putative function	Nearest match	e-value
Developmental libraries
Contig 175	102	S0S1	Metallothionein-like protein	AY833008	7E-24
Contig 172	49	S0S1	Large subunit rRNA	DQ008809	1E-73
Contig 180	40	S2S4	Metallothionein type II	AF039003	9E-27
Contig 19	30	S0S1	Metallothionein type II	AF039003	9E-27
Contig 239	19	S0S1	Metallothionein type II	AF147786	4E-23
Contig 252	19	S2S4	Metallothionein type II	AF039003	2E-28
Contig 35	19	S2S4	Metallothionein-like protein	AF279655	3E-30
Contig 158	17	SSH0-2	Putative cell wall P8 protein	AC146631	0.0009
Contig 203	16	S0S1	Cytochrome b6/f complex subunit VIII	BAF64867	0.001
Contig 198	15	S0S1	Ubiquitin carrier-like protein	DQ294271	1E-101
Contig 150	13	SSH0-2	GSDL-motif lipase	AY491975	6E-62
Contig 242	13	S2S4	Metallothionein type I	AF039002	1E-22
Contig 214	11	S0S1	40S ribosomal protein S17 (RPS17D)	AT5G04800	3E-64
Contig 128	10	SSH2-45	Dihydroflavonol-4-reductase	AY374471	1E-54
Contig 221	10	S2S4	Large subunit rRNA gene	DQ008808	0
Contig 45	10	SSH2-0	Aldehyde decarbonylase (epicuticular wax associated)	ATHCER1	2E-56
Stress libraries
Contig 175	48	COLD	Metallothionein-like protein	AY833008	7E-24
Contig 172	22	COLD	Large subunit rRNA	DQ008809	1E-73
Contig 19	15	COLD	Metallothionein type II	AF039003	9E-27
Contig 242	12	COLD	Metallothionein type I	AF039002	1E-22
Contig 263	12	COLD	Arabidopsis unknown protein/CXE carboxylesterase	AT1G47480	0
Contig 180	9	COLD	Metallothionein type II	AF039003	9E-27
Contig 186	5	COLD	No significant BLAST match
Contig 187	4	COLD	Elongation factor-1 alpha 2
Contig 239	4	COLD	Metallothionein-like protein (ML2)	AF147786	4E-23
Contig 252	4	COLD	Metallothionein type II	AF039003	2E-28
Contig 154	3	COLD	Ubiquitin precursor-like	EU249995	E-116
Contig 193	3	COLD	Glutathione transferase	AJ441055	4E-72
Contig 35	3	COLD	Metallothionein-like protein	AF279655	3E-30

Petal libraries: S0S1, S2S4 are non-subtracted libraries from combined stages S0 and S2, and S2 and S4 respectively; SSH0-2 and SSH2-45 are subtracted libraries of stage S2 from S0 and combined stages S4 and S5 from S2, respectively; COLD library combined a 3 d cold treatment followed by a 3 h or 24 h refreshment period (see Materials and methods for further details).

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