ZIKV Inhibition and Cytotoxicity Characteristics of Selected Nucleoside Analogues
| Compound | Structure | EC50, µM, Mean ± SDa,b | Apparent EC50, µM, Mean ± SDa,c | CC50, µMa | SId |
|---|---|---|---|---|---|
| 2′-C-methyladenosine |  | 5.26 ± 0.12 | 26.41 ± 1.14 | >100f | >19.01 |
| 7-deaza-2′-C-methyladenosine |  | 8.92 ± 3.32 | 42.87 ± 1.88 | >100 | >11.21 |
| 2′-C-methylguanosine |  | 22.25 ± 0.03 | 71.23 ± 4.11 | >100 | >4.49 |
| 2′-C-methylcytidine |  | 10.51 ± 0.02 | 61.93 ± 3.76 | >100g | >9.51 |
| 2′-C-methyluridine |  | 45.45 ± 0.64 | >100e | >100 | >2.20 |
| Compound | Structure | EC50, µM, Mean ± SDa,b | Apparent EC50, µM, Mean ± SDa,c | CC50, µMa | SId |
|---|---|---|---|---|---|
| 2′-C-methyladenosine | | 5.26 ± 0.12 | 26.41 ± 1.14 | >100f | >19.01 |
| 7-deaza-2′-C-methyladenosine | | 8.92 ± 3.32 | 42.87 ± 1.88 | >100 | >11.21 |
| 2′-C-methylguanosine | | 22.25 ± 0.03 | 71.23 ± 4.11 | >100 | >4.49 |
| 2′-C-methylcytidine | | 10.51 ± 0.02 | 61.93 ± 3.76 | >100g | >9.51 |
| 2′-C-methyluridine | | 45.45 ± 0.64 | >100e | >100 | >2.20 |
Abbreviations: CC50, 50% cytotoxic concentration; EC50, 50% effective concentration; SD, standard deviation; ZIKV, Zika virus.
a Determined from 3 independent experiments.
b Calculated as a 50% reduction of virus titers, using the Reed-Muench method.
c Calculated as a point of inflection from dose-response curves, using log-transformed viral titers.
d The selectivity index (SI) is calculated as CC50/EC50.
e Treatment with 100 µM of 2′-CMU reduced virus titers 102-fold compared to a mock-treated culture.
f Treatment of cell culture with 2′-CMA at a concentration of 100 µM led to a reduction in cell viability to 79.1%.
g Treatment of cell culture with 2′-CMC at concentration of 100 µM led to a reduction in cell viability to 69.3%.
ZIKV Inhibition and Cytotoxicity Characteristics of Selected Nucleoside Analogues
| Compound | Structure | EC50, µM, Mean ± SDa,b | Apparent EC50, µM, Mean ± SDa,c | CC50, µMa | SId |
|---|---|---|---|---|---|
| 2′-C-methyladenosine | | 5.26 ± 0.12 | 26.41 ± 1.14 | >100f | >19.01 |
| 7-deaza-2′-C-methyladenosine | | 8.92 ± 3.32 | 42.87 ± 1.88 | >100 | >11.21 |
| 2′-C-methylguanosine | | 22.25 ± 0.03 | 71.23 ± 4.11 | >100 | >4.49 |
| 2′-C-methylcytidine | | 10.51 ± 0.02 | 61.93 ± 3.76 | >100g | >9.51 |
| 2′-C-methyluridine | | 45.45 ± 0.64 | >100e | >100 | >2.20 |
| Compound | Structure | EC50, µM, Mean ± SDa,b | Apparent EC50, µM, Mean ± SDa,c | CC50, µMa | SId |
|---|---|---|---|---|---|
| 2′-C-methyladenosine | | 5.26 ± 0.12 | 26.41 ± 1.14 | >100f | >19.01 |
| 7-deaza-2′-C-methyladenosine | | 8.92 ± 3.32 | 42.87 ± 1.88 | >100 | >11.21 |
| 2′-C-methylguanosine | | 22.25 ± 0.03 | 71.23 ± 4.11 | >100 | >4.49 |
| 2′-C-methylcytidine | | 10.51 ± 0.02 | 61.93 ± 3.76 | >100g | >9.51 |
| 2′-C-methyluridine | | 45.45 ± 0.64 | >100e | >100 | >2.20 |
Abbreviations: CC50, 50% cytotoxic concentration; EC50, 50% effective concentration; SD, standard deviation; ZIKV, Zika virus.
a Determined from 3 independent experiments.
b Calculated as a 50% reduction of virus titers, using the Reed-Muench method.
c Calculated as a point of inflection from dose-response curves, using log-transformed viral titers.
d The selectivity index (SI) is calculated as CC50/EC50.
e Treatment with 100 µM of 2′-CMU reduced virus titers 102-fold compared to a mock-treated culture.
f Treatment of cell culture with 2′-CMA at a concentration of 100 µM led to a reduction in cell viability to 79.1%.
g Treatment of cell culture with 2′-CMC at concentration of 100 µM led to a reduction in cell viability to 69.3%.
