Creatine levels and creatine kinase activity in cells cultured with and without creatine
| . | 3T3-CrT cells . | HL-1 cells . | ||
|---|---|---|---|---|
| Control . | +5 mM Cr . | Control . | +5 mM Cr . | |
| Creatine (nmol/mg protein) | 33.8 ± 0.5 | 54.7 ± 0.5 | 20.0 ± 0.4 | 52.0 ± 0.8 |
| Phosphocreatine (nmol/mg Pr) | 2.4 ± 0.1 | 1.8 ± 0.1 | 5.2 ± 0.2 | 16.5 ± 0.4 |
| Total CK activity (IU CK/mg Pr) | ND | ND | 0.32 ± 0.01 | 0.31 ± 0.01 |
| Mito-CK | ND | ND | 0.21 ± 0.01 | 0.19 ± 0.00 |
| MM-CK | ND | ND | 0.12 ± 0.02 | 0.07 ± 0.01 |
| MB-CK | ND | ND | ND | 0.02 ± 0.00 |
| BB-CK | ND | ND | ND | 0.04 ± 0.00 |
| . | 3T3-CrT cells . | HL-1 cells . | ||
|---|---|---|---|---|
| Control . | +5 mM Cr . | Control . | +5 mM Cr . | |
| Creatine (nmol/mg protein) | 33.8 ± 0.5 | 54.7 ± 0.5 | 20.0 ± 0.4 | 52.0 ± 0.8 |
| Phosphocreatine (nmol/mg Pr) | 2.4 ± 0.1 | 1.8 ± 0.1 | 5.2 ± 0.2 | 16.5 ± 0.4 |
| Total CK activity (IU CK/mg Pr) | ND | ND | 0.32 ± 0.01 | 0.31 ± 0.01 |
| Mito-CK | ND | ND | 0.21 ± 0.01 | 0.19 ± 0.00 |
| MM-CK | ND | ND | 0.12 ± 0.02 | 0.07 ± 0.01 |
| MB-CK | ND | ND | ND | 0.02 ± 0.00 |
| BB-CK | ND | ND | ND | 0.04 ± 0.00 |
Data are mean ± SD. CK, creatine kinase; ND denotes below limits of detection. Cells incubated in normal culture media (control) or supplemented with 5 mM creatine monohydrate for 24 h.
Creatine levels and creatine kinase activity in cells cultured with and without creatine
| . | 3T3-CrT cells . | HL-1 cells . | ||
|---|---|---|---|---|
| Control . | +5 mM Cr . | Control . | +5 mM Cr . | |
| Creatine (nmol/mg protein) | 33.8 ± 0.5 | 54.7 ± 0.5 | 20.0 ± 0.4 | 52.0 ± 0.8 |
| Phosphocreatine (nmol/mg Pr) | 2.4 ± 0.1 | 1.8 ± 0.1 | 5.2 ± 0.2 | 16.5 ± 0.4 |
| Total CK activity (IU CK/mg Pr) | ND | ND | 0.32 ± 0.01 | 0.31 ± 0.01 |
| Mito-CK | ND | ND | 0.21 ± 0.01 | 0.19 ± 0.00 |
| MM-CK | ND | ND | 0.12 ± 0.02 | 0.07 ± 0.01 |
| MB-CK | ND | ND | ND | 0.02 ± 0.00 |
| BB-CK | ND | ND | ND | 0.04 ± 0.00 |
| . | 3T3-CrT cells . | HL-1 cells . | ||
|---|---|---|---|---|
| Control . | +5 mM Cr . | Control . | +5 mM Cr . | |
| Creatine (nmol/mg protein) | 33.8 ± 0.5 | 54.7 ± 0.5 | 20.0 ± 0.4 | 52.0 ± 0.8 |
| Phosphocreatine (nmol/mg Pr) | 2.4 ± 0.1 | 1.8 ± 0.1 | 5.2 ± 0.2 | 16.5 ± 0.4 |
| Total CK activity (IU CK/mg Pr) | ND | ND | 0.32 ± 0.01 | 0.31 ± 0.01 |
| Mito-CK | ND | ND | 0.21 ± 0.01 | 0.19 ± 0.00 |
| MM-CK | ND | ND | 0.12 ± 0.02 | 0.07 ± 0.01 |
| MB-CK | ND | ND | ND | 0.02 ± 0.00 |
| BB-CK | ND | ND | ND | 0.04 ± 0.00 |
Data are mean ± SD. CK, creatine kinase; ND denotes below limits of detection. Cells incubated in normal culture media (control) or supplemented with 5 mM creatine monohydrate for 24 h.
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