| . | Hrq1 . | RecQ4 . | ||
|---|---|---|---|---|
| Substrate . | Binding Kd (nM) . | Unwinding KM (nM) . | Binding Kd (nM) . | Unwinding KM (nM) . |
| Poly(dT) ssDNA1 | 3.45 ± 0.19 | N/A | 2.38 ± 0.15 | N/A |
| Random Fork | N/D | 113.9 ± 19.5 | 107.7 ± 32.5 | 14.7 ± 1.1 |
| 3΄ tail | 140.7 ± 59.4 | 111.4 ± 43.4 | 21.8 ± 4.2 | 98.1 ± 17.8 |
| 5΄ tail | 154.5 ± 44.6 | - | 101.6 ± 29.4 | - |
| Blunt dsDNA | - | - | - | - |
| Bubble | 36.1 ± 4.9 | 23.6 ± 2.2 | 16.8 ± 2.3 | 143 ± 56.1 |
| HJ | 79.2 ± 22.7 | - | 90.1 ± 23.4 | - |
| D-loop | 9.9 ± 0.7 | 1.6 ± 0.1 | 12.6 ± 1.8 | 5.7 ± 0.8 |
| G4 | 17.6 ± 2.2 | 14.1 ± 0.9 | 12.3 ± 2.5 | 38.4 ± 8.7 |
| Poly(dT) Fork | 6.5 ± 0.5 | 12.6 ± 1.7 | 50.9 ± 10.4 | 21.7 ± 1.1 |
| Yeast Tel Fork | 3.5 ± 0.4 | 2.0 ± 0.2 | 18.5 ± 3.1 | 9.2 ± 1.6 |
| Human Tel Fork | 15.7 ± 1.7 | 23.0 ± 1.5 | 39.7 ± 8.3 | 35.4 ± 5.4 |
| . | Hrq1 . | RecQ4 . | ||
|---|---|---|---|---|
| Substrate . | Binding Kd (nM) . | Unwinding KM (nM) . | Binding Kd (nM) . | Unwinding KM (nM) . |
| Poly(dT) ssDNA1 | 3.45 ± 0.19 | N/A | 2.38 ± 0.15 | N/A |
| Random Fork | N/D | 113.9 ± 19.5 | 107.7 ± 32.5 | 14.7 ± 1.1 |
| 3΄ tail | 140.7 ± 59.4 | 111.4 ± 43.4 | 21.8 ± 4.2 | 98.1 ± 17.8 |
| 5΄ tail | 154.5 ± 44.6 | - | 101.6 ± 29.4 | - |
| Blunt dsDNA | - | - | - | - |
| Bubble | 36.1 ± 4.9 | 23.6 ± 2.2 | 16.8 ± 2.3 | 143 ± 56.1 |
| HJ | 79.2 ± 22.7 | - | 90.1 ± 23.4 | - |
| D-loop | 9.9 ± 0.7 | 1.6 ± 0.1 | 12.6 ± 1.8 | 5.7 ± 0.8 |
| G4 | 17.6 ± 2.2 | 14.1 ± 0.9 | 12.3 ± 2.5 | 38.4 ± 8.7 |
| Poly(dT) Fork | 6.5 ± 0.5 | 12.6 ± 1.7 | 50.9 ± 10.4 | 21.7 ± 1.1 |
| Yeast Tel Fork | 3.5 ± 0.4 | 2.0 ± 0.2 | 18.5 ± 3.1 | 9.2 ± 1.6 |
| Human Tel Fork | 15.7 ± 1.7 | 23.0 ± 1.5 | 39.7 ± 8.3 | 35.4 ± 5.4 |
1Abbreviations used: ssDNA, single-stranded DNA; N/D, not determined; N/A, not applicable; dsDNA, double-stranded DNA; HJ, Holliday junction; G4, G-quadruplex; and Tel, telomere repeat sequence.
| . | Hrq1 . | RecQ4 . | ||
|---|---|---|---|---|
| Substrate . | Binding Kd (nM) . | Unwinding KM (nM) . | Binding Kd (nM) . | Unwinding KM (nM) . |
| Poly(dT) ssDNA1 | 3.45 ± 0.19 | N/A | 2.38 ± 0.15 | N/A |
| Random Fork | N/D | 113.9 ± 19.5 | 107.7 ± 32.5 | 14.7 ± 1.1 |
| 3΄ tail | 140.7 ± 59.4 | 111.4 ± 43.4 | 21.8 ± 4.2 | 98.1 ± 17.8 |
| 5΄ tail | 154.5 ± 44.6 | - | 101.6 ± 29.4 | - |
| Blunt dsDNA | - | - | - | - |
| Bubble | 36.1 ± 4.9 | 23.6 ± 2.2 | 16.8 ± 2.3 | 143 ± 56.1 |
| HJ | 79.2 ± 22.7 | - | 90.1 ± 23.4 | - |
| D-loop | 9.9 ± 0.7 | 1.6 ± 0.1 | 12.6 ± 1.8 | 5.7 ± 0.8 |
| G4 | 17.6 ± 2.2 | 14.1 ± 0.9 | 12.3 ± 2.5 | 38.4 ± 8.7 |
| Poly(dT) Fork | 6.5 ± 0.5 | 12.6 ± 1.7 | 50.9 ± 10.4 | 21.7 ± 1.1 |
| Yeast Tel Fork | 3.5 ± 0.4 | 2.0 ± 0.2 | 18.5 ± 3.1 | 9.2 ± 1.6 |
| Human Tel Fork | 15.7 ± 1.7 | 23.0 ± 1.5 | 39.7 ± 8.3 | 35.4 ± 5.4 |
| . | Hrq1 . | RecQ4 . | ||
|---|---|---|---|---|
| Substrate . | Binding Kd (nM) . | Unwinding KM (nM) . | Binding Kd (nM) . | Unwinding KM (nM) . |
| Poly(dT) ssDNA1 | 3.45 ± 0.19 | N/A | 2.38 ± 0.15 | N/A |
| Random Fork | N/D | 113.9 ± 19.5 | 107.7 ± 32.5 | 14.7 ± 1.1 |
| 3΄ tail | 140.7 ± 59.4 | 111.4 ± 43.4 | 21.8 ± 4.2 | 98.1 ± 17.8 |
| 5΄ tail | 154.5 ± 44.6 | - | 101.6 ± 29.4 | - |
| Blunt dsDNA | - | - | - | - |
| Bubble | 36.1 ± 4.9 | 23.6 ± 2.2 | 16.8 ± 2.3 | 143 ± 56.1 |
| HJ | 79.2 ± 22.7 | - | 90.1 ± 23.4 | - |
| D-loop | 9.9 ± 0.7 | 1.6 ± 0.1 | 12.6 ± 1.8 | 5.7 ± 0.8 |
| G4 | 17.6 ± 2.2 | 14.1 ± 0.9 | 12.3 ± 2.5 | 38.4 ± 8.7 |
| Poly(dT) Fork | 6.5 ± 0.5 | 12.6 ± 1.7 | 50.9 ± 10.4 | 21.7 ± 1.1 |
| Yeast Tel Fork | 3.5 ± 0.4 | 2.0 ± 0.2 | 18.5 ± 3.1 | 9.2 ± 1.6 |
| Human Tel Fork | 15.7 ± 1.7 | 23.0 ± 1.5 | 39.7 ± 8.3 | 35.4 ± 5.4 |
1Abbreviations used: ssDNA, single-stranded DNA; N/D, not determined; N/A, not applicable; dsDNA, double-stranded DNA; HJ, Holliday junction; G4, G-quadruplex; and Tel, telomere repeat sequence.
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