Table 1 Open in new tab LC-MS/MS...

Protein

Accession No.

Mol. wt (kDa)

MASCOT score

No. of hit peptides

Sequence coverage (%)

TM prediction^a

Innate immunity

Phosphatidylinositol-4-phosphate 5-kinase family protein, low similarity to phosphatidylinositol 3,5-kinase

gi|115467450

175

Disease resistance protein RPM1 (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists

gi|125535287

140

NB-ARC domain, putative

gi|115484771

110

Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D

gi|108864453

105

186

Leucine-rich repeat transmembrane protein kinase, putative protein kinase Xa21

gi|125532138

103

Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D

gi|115488934

100

108

Heat shock protein 90

gi|6863054

Serine/threonine protein kinases active site signature

gi|32988727

Atypical harpin-like kinase MARK

gi|13324792

548

Heat shock cognate 70 kDa protein

gi|115452223

305

Nodulation harpin kinase-like protein

gi|115478014

129

Putative elicitor inducible beta-1,3-glucanase NtEIG-E76

gi|115439837

100

Putative disease resistance protein

gi|115434084

Putative protein kinase serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)

gi|115456539

Chitin elicitor-binding protein

gi|108860575

218

Putative Pto kinase interactor 1

gi|115461953

Receptor for activated C kinase 1A (RACK1A)

gi|115439261

115

Putative hypersensitive-induced response protein

gi|115465785

477

Band 7 family protein strong similarity to hypersensitive-induced response protein

gi|115482396

212

Hypersensitive-induced response protein

gi|14150732

106

Hypersensitive-induced response protein

gi|115476296

337

Small GTP-binding protein OsRac2

gi|115464861

Harpin-induced family protein (YLS9)/HIN1 family protein

gi|115461434

179

Harpin-induced gene 1 homolog

gi|2801538

Harpin-induced protein 1-containing protein

gi|115487442

154

Harpin-induced family protein/NDR1/HIN1-like protein 3 similar to harpin-induced protein hin1

gi|32993161

Harpin-induced protein-related/HIN1-related/harpin-responsive protein-related weak similarity to hin1

gi|115472507

Receptor

Putative receptor-like protein kinase

gi|115467902

Leucine-rich repeat family protein, expressed

gi|108863916

Leucine-rich repeat transmembrane protein kinase, putative

gi|116012935

211

Somatic embryogenesis protein kinase 1

gi|52854318

Brassinosteroid-insensitive 1-associated harpin kinase 1 precursor (somatic embryogenesis harpin-like kinase 3)

gi|115458750

Leucine-rich repeat transmembrane protein kinase, putative similar to CLV1 harpin kinase

gi|115459088

Protein kinase family protein, putative, expressed

gi|115450539

131

Putative kinase-like protein TMKL1 precursor

gi|115438420

Leucine-rich repeat family protein similar to leucine-rich repeat protein SHOC-2 (Ras-binding protein Sur-8)

gi|32975440

Peptidoglycan-binding LysM domain-containing protein contains Pfam profile PF01476

gi|115480519

164

SNARE

Syntaxin 121, putative, expressed

gi|115455787

Putative syntaxin-related protein (SYP132)

gi|115470721

189

Synaptobrevin family protein similar to vesicle-associated membrane protein 7

gi|125557559

156

Novel plant SNARE 11

gi|115453189

Syntaxin 71 (SYP71) identified as syntaxin of plants 71

gi|115465329

199

Vesicle-associated membrane protein 724

gi|115456011

Small G-protein and others

WD-40 repeat family protein contains three WD-40 repeats (PF00400); some similarity to s-tomosyn isoform

gi|156765316

168

343

WD-40 repeat family protein contains three Pfam PF00400: WD domain, G-beta repeats

gi|37988349

148

582

Probable GTP-binding protein

gi|115437816

Transducin family protein/WD-40 repeat family protein

gi|108706574

606

Transducin family protein/WD-40 repeat family protein contains six WD-40 repeats; similar to cell cycle control protein cwf8

gi|115482422

GTP-binding protein GTP1

gi|115439941

ADP-ribosylation factor, putative, expressed

gi|108711707

Protein

Accession No.

Mol. wt (kDa)

MASCOT score

No. of hit peptides

Sequence coverage (%)

TM prediction^a

Innate immunity

Phosphatidylinositol-4-phosphate 5-kinase family protein, low similarity to phosphatidylinositol 3,5-kinase

gi|115467450

175

Disease resistance protein RPM1 (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists

gi|125535287

140

NB-ARC domain, putative

gi|115484771

110

Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D

gi|108864453

105

186

Leucine-rich repeat transmembrane protein kinase, putative protein kinase Xa21

gi|125532138

103

Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D

gi|115488934

100

108

Heat shock protein 90

gi|6863054

Serine/threonine protein kinases active site signature

gi|32988727

Atypical harpin-like kinase MARK

gi|13324792

548

Heat shock cognate 70 kDa protein

gi|115452223

305

Nodulation harpin kinase-like protein

gi|115478014

129

Putative elicitor inducible beta-1,3-glucanase NtEIG-E76

gi|115439837

100

Putative disease resistance protein

gi|115434084

Putative protein kinase serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)

gi|115456539

Chitin elicitor-binding protein

gi|108860575

218

Putative Pto kinase interactor 1

gi|115461953

Receptor for activated C kinase 1A (RACK1A)

gi|115439261

115

Putative hypersensitive-induced response protein

gi|115465785

477

Band 7 family protein strong similarity to hypersensitive-induced response protein

gi|115482396

212

Hypersensitive-induced response protein

gi|14150732

106

Hypersensitive-induced response protein

gi|115476296

337

Small GTP-binding protein OsRac2

gi|115464861

Harpin-induced family protein (YLS9)/HIN1 family protein

gi|115461434

179

Harpin-induced gene 1 homolog

gi|2801538

Harpin-induced protein 1-containing protein

gi|115487442

154

Harpin-induced family protein/NDR1/HIN1-like protein 3 similar to harpin-induced protein hin1

gi|32993161

Harpin-induced protein-related/HIN1-related/harpin-responsive protein-related weak similarity to hin1

gi|115472507

Receptor

Putative receptor-like protein kinase

gi|115467902

Leucine-rich repeat family protein, expressed

gi|108863916

Leucine-rich repeat transmembrane protein kinase, putative

gi|116012935

211

Somatic embryogenesis protein kinase 1

gi|52854318

Brassinosteroid-insensitive 1-associated harpin kinase 1 precursor (somatic embryogenesis harpin-like kinase 3)

gi|115458750

Leucine-rich repeat transmembrane protein kinase, putative similar to CLV1 harpin kinase

gi|115459088

Protein kinase family protein, putative, expressed

gi|115450539

131

Putative kinase-like protein TMKL1 precursor

gi|115438420

Leucine-rich repeat family protein similar to leucine-rich repeat protein SHOC-2 (Ras-binding protein Sur-8)

gi|32975440

Peptidoglycan-binding LysM domain-containing protein contains Pfam profile PF01476

gi|115480519

164

SNARE

Syntaxin 121, putative, expressed

gi|115455787

Putative syntaxin-related protein (SYP132)

gi|115470721

189

Synaptobrevin family protein similar to vesicle-associated membrane protein 7

gi|125557559

156

Novel plant SNARE 11

gi|115453189

Syntaxin 71 (SYP71) identified as syntaxin of plants 71

gi|115465329

199

Vesicle-associated membrane protein 724

gi|115456011

Small G-protein and others

WD-40 repeat family protein contains three WD-40 repeats (PF00400); some similarity to s-tomosyn isoform

gi|156765316

168

343

WD-40 repeat family protein contains three Pfam PF00400: WD domain, G-beta repeats

gi|37988349

148

582

Probable GTP-binding protein

gi|115437816

Transducin family protein/WD-40 repeat family protein

gi|108706574

606

Transducin family protein/WD-40 repeat family protein contains six WD-40 repeats; similar to cell cycle control protein cwf8

gi|115482422

GTP-binding protein GTP1

gi|115439941

ADP-ribosylation factor, putative, expressed

gi|108711707

Table 1

Open in new tab

LC-MS/MS identification of proteins in DRMs isolated from myc-tagged CA-OsRac1-transformed rice suspension cells

	Protein	Accession No.	Mol. wt (kDa)	MASCOT score	No. of hit peptides	Sequence coverage (%)	TM prediction^a
Innate immunity	Phosphatidylinositol-4-phosphate 5-kinase family protein, low similarity to phosphatidylinositol 3,5-kinase	gi\|115467450	175	61	2	1	0
	Disease resistance protein RPM1 (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists	gi\|125535287	140	61	2	1	0
	NB-ARC domain, putative	gi\|115484771	110	58	2	2	0
	Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D	gi\|108864453	105	186	12	23	5
	Leucine-rich repeat transmembrane protein kinase, putative protein kinase Xa21	gi\|125532138	103	60	2	1	2
	Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D	gi\|115488934	100	108	9	14	5
	Heat shock protein 90	gi\|6863054	93	80	4	6	0
	Serine/threonine protein kinases active site signature	gi\|32988727	76	62	2	3	1
	Atypical harpin-like kinase MARK	gi\|13324792	72	548	14	32	1
	Heat shock cognate 70 kDa protein	gi\|115452223	71	305	10	14	0
	Nodulation harpin kinase-like protein	gi\|115478014	59	129	4	9	1
	Putative elicitor inducible beta-1,3-glucanase NtEIG-E76	gi\|115439837	54	100	9	18	1
	Putative disease resistance protein	gi\|115434084	48	75	2	5	0
	Putative protein kinase serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)	gi\|115456539	41	54	2	9	0
	Chitin elicitor-binding protein	gi\|108860575	40	218	8	27	2
	Putative Pto kinase interactor 1	gi\|115461953	39	75	6	19	0
	Receptor for activated C kinase 1A (RACK1A)	gi\|115439261	36	115	3	13	0
	Putative hypersensitive-induced response protein	gi\|115465785	32	477	25	48	0
	Band 7 family protein strong similarity to hypersensitive-induced response protein	gi\|115482396	32	212	11	32	0
	Hypersensitive-induced response protein	gi\|14150732	31	106	4	9	0
	Hypersensitive-induced response protein	gi\|115476296	31	337	21	40	0
	Small GTP-binding protein OsRac2	gi\|115464861	24	59	2	16	0
	Harpin-induced family protein (YLS9)/HIN1 family protein	gi\|115461434	22	179	3	25	2
	Harpin-induced gene 1 homolog	gi\|2801538	22	92	6	30	2
	Harpin-induced protein 1-containing protein	gi\|115487442	21	154	6	27	2
	Harpin-induced family protein/NDR1/HIN1-like protein 3 similar to harpin-induced protein hin1	gi\|32993161	21	93	3	23	2
	Harpin-induced protein-related/HIN1-related/harpin-responsive protein-related weak similarity to hin1	gi\|115472507	19	76	2	11	0
Receptor	Putative receptor-like protein kinase	gi\|115467902	94	61	5	6	2
	Leucine-rich repeat family protein, expressed	gi\|108863916	84	81	9	12	2
	Leucine-rich repeat transmembrane protein kinase, putative	gi\|116012935	74	211	4	11	1
	Somatic embryogenesis protein kinase 1	gi\|52854318	70	59	5	8	2
	Brassinosteroid-insensitive 1-associated harpin kinase 1 precursor (somatic embryogenesis harpin-like kinase 3)	gi\|115458750	70	59	4	5	1
	Leucine-rich repeat transmembrane protein kinase, putative similar to CLV1 harpin kinase	gi\|115459088	66	59	3	7	1
	Protein kinase family protein, putative, expressed	gi\|115450539	59	131	5	13	0
	Putative kinase-like protein TMKL1 precursor	gi\|115438420	54	81	2	4	2
	Leucine-rich repeat family protein similar to leucine-rich repeat protein SHOC-2 (Ras-binding protein Sur-8)	gi\|32975440	51	79	2	5	0
	Peptidoglycan-binding LysM domain-containing protein contains Pfam profile PF01476	gi\|115480519	39	164	4	14	2
SNARE	Syntaxin 121, putative, expressed	gi\|115455787	36	56	2	5	1
	Putative syntaxin-related protein (SYP132)	gi\|115470721	34	189	6	20	1
	Synaptobrevin family protein similar to vesicle-associated membrane protein 7	gi\|125557559	32	156	9	25	2
	Novel plant SNARE 11	gi\|115453189	31	65	12	30	1
	Syntaxin 71 (SYP71) identified as syntaxin of plants 71	gi\|115465329	30	199	14	47	1
	Vesicle-associated membrane protein 724	gi\|115456011	25	53	2	11	1
Small G-protein and others	WD-40 repeat family protein contains three WD-40 repeats (PF00400); some similarity to s-tomosyn isoform	gi\|156765316	168	343	21	20	0
	WD-40 repeat family protein contains three Pfam PF00400: WD domain, G-beta repeats	gi\|37988349	148	582	29	25	0
	Probable GTP-binding protein	gi\|115437816	90	89	2	5	0
	Transducin family protein/WD-40 repeat family protein	gi\|108706574	82	606	33	34	0
	Transducin family protein/WD-40 repeat family protein contains six WD-40 repeats; similar to cell cycle control protein cwf8	gi\|115482422	58	64	8	21	0
	GTP-binding protein GTP1	gi\|115439941	24	59	3	8	0
	ADP-ribosylation factor, putative, expressed	gi\|108711707	22	87	3	18	0

	Protein	Accession No.	Mol. wt (kDa)	MASCOT score	No. of hit peptides	Sequence coverage (%)	TM prediction^a
Innate immunity	Phosphatidylinositol-4-phosphate 5-kinase family protein, low similarity to phosphatidylinositol 3,5-kinase	gi\|115467450	175	61	2	1	0
	Disease resistance protein RPM1 (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists	gi\|125535287	140	61	2	1	0
	NB-ARC domain, putative	gi\|115484771	110	58	2	2	0
	Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D	gi\|108864453	105	186	12	23	5
	Leucine-rich repeat transmembrane protein kinase, putative protein kinase Xa21	gi\|125532138	103	60	2	1	2
	Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D	gi\|115488934	100	108	9	14	5
	Heat shock protein 90	gi\|6863054	93	80	4	6	0
	Serine/threonine protein kinases active site signature	gi\|32988727	76	62	2	3	1
	Atypical harpin-like kinase MARK	gi\|13324792	72	548	14	32	1
	Heat shock cognate 70 kDa protein	gi\|115452223	71	305	10	14	0
	Nodulation harpin kinase-like protein	gi\|115478014	59	129	4	9	1
	Putative elicitor inducible beta-1,3-glucanase NtEIG-E76	gi\|115439837	54	100	9	18	1
	Putative disease resistance protein	gi\|115434084	48	75	2	5	0
	Putative protein kinase serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)	gi\|115456539	41	54	2	9	0
	Chitin elicitor-binding protein	gi\|108860575	40	218	8	27	2
	Putative Pto kinase interactor 1	gi\|115461953	39	75	6	19	0
	Receptor for activated C kinase 1A (RACK1A)	gi\|115439261	36	115	3	13	0
	Putative hypersensitive-induced response protein	gi\|115465785	32	477	25	48	0
	Band 7 family protein strong similarity to hypersensitive-induced response protein	gi\|115482396	32	212	11	32	0
	Hypersensitive-induced response protein	gi\|14150732	31	106	4	9	0
	Hypersensitive-induced response protein	gi\|115476296	31	337	21	40	0
	Small GTP-binding protein OsRac2	gi\|115464861	24	59	2	16	0
	Harpin-induced family protein (YLS9)/HIN1 family protein	gi\|115461434	22	179	3	25	2
	Harpin-induced gene 1 homolog	gi\|2801538	22	92	6	30	2
	Harpin-induced protein 1-containing protein	gi\|115487442	21	154	6	27	2
	Harpin-induced family protein/NDR1/HIN1-like protein 3 similar to harpin-induced protein hin1	gi\|32993161	21	93	3	23	2
	Harpin-induced protein-related/HIN1-related/harpin-responsive protein-related weak similarity to hin1	gi\|115472507	19	76	2	11	0
Receptor	Putative receptor-like protein kinase	gi\|115467902	94	61	5	6	2
	Leucine-rich repeat family protein, expressed	gi\|108863916	84	81	9	12	2
	Leucine-rich repeat transmembrane protein kinase, putative	gi\|116012935	74	211	4	11	1
	Somatic embryogenesis protein kinase 1	gi\|52854318	70	59	5	8	2
	Brassinosteroid-insensitive 1-associated harpin kinase 1 precursor (somatic embryogenesis harpin-like kinase 3)	gi\|115458750	70	59	4	5	1
	Leucine-rich repeat transmembrane protein kinase, putative similar to CLV1 harpin kinase	gi\|115459088	66	59	3	7	1
	Protein kinase family protein, putative, expressed	gi\|115450539	59	131	5	13	0
	Putative kinase-like protein TMKL1 precursor	gi\|115438420	54	81	2	4	2
	Leucine-rich repeat family protein similar to leucine-rich repeat protein SHOC-2 (Ras-binding protein Sur-8)	gi\|32975440	51	79	2	5	0
	Peptidoglycan-binding LysM domain-containing protein contains Pfam profile PF01476	gi\|115480519	39	164	4	14	2
SNARE	Syntaxin 121, putative, expressed	gi\|115455787	36	56	2	5	1
	Putative syntaxin-related protein (SYP132)	gi\|115470721	34	189	6	20	1
	Synaptobrevin family protein similar to vesicle-associated membrane protein 7	gi\|125557559	32	156	9	25	2
	Novel plant SNARE 11	gi\|115453189	31	65	12	30	1
	Syntaxin 71 (SYP71) identified as syntaxin of plants 71	gi\|115465329	30	199	14	47	1
	Vesicle-associated membrane protein 724	gi\|115456011	25	53	2	11	1
Small G-protein and others	WD-40 repeat family protein contains three WD-40 repeats (PF00400); some similarity to s-tomosyn isoform	gi\|156765316	168	343	21	20	0
	WD-40 repeat family protein contains three Pfam PF00400: WD domain, G-beta repeats	gi\|37988349	148	582	29	25	0
	Probable GTP-binding protein	gi\|115437816	90	89	2	5	0
	Transducin family protein/WD-40 repeat family protein	gi\|108706574	82	606	33	34	0
	Transducin family protein/WD-40 repeat family protein contains six WD-40 repeats; similar to cell cycle control protein cwf8	gi\|115482422	58	64	8	21	0
	GTP-binding protein GTP1	gi\|115439941	24	59	3	8	0
	ADP-ribosylation factor, putative, expressed	gi\|108711707	22	87	3	18	0

^aThe numbers of putative transmembrane domains were predicted by SOSUI.

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