Table 4

Origin of Agave-associated bacterial strains grown in N-free culture media

Strain IDAgave speciesCommunity typeConfirmed ARA activitySILVA Taxonomic lineage (NCBI best hit accession number)DGGE cluster
MJ02A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ03A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ04A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_104933)1
MJ05A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ06A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ07A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104934)1
MJ08A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ09A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ10A. salmianaRhizosphere (rz)NoBacteria. (NR_116033) (partial sequence)
MJ11A. salmianaRhizosphere (rz)YesBacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus (NR_115953)2
MJ13A. salmianaRhizosphere (rz)NoBacteria. (NR_117704)
MJ14A. salmianaRhizosphere (rz)YesBacteria; Actinobacteria; Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium. (NR_044937)
MJ15A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704)
MJ17A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ18A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704) (partial sequence)
MJ19A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ20A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ22A. salmianaPhyllosphere (p)YesBacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae. (NR_109297)
Strain IDAgave speciesCommunity typeConfirmed ARA activitySILVA Taxonomic lineage (NCBI best hit accession number)DGGE cluster
MJ02A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ03A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ04A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_104933)1
MJ05A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ06A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ07A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104934)1
MJ08A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ09A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ10A. salmianaRhizosphere (rz)NoBacteria. (NR_116033) (partial sequence)
MJ11A. salmianaRhizosphere (rz)YesBacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus (NR_115953)2
MJ13A. salmianaRhizosphere (rz)NoBacteria. (NR_117704)
MJ14A. salmianaRhizosphere (rz)YesBacteria; Actinobacteria; Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium. (NR_044937)
MJ15A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704)
MJ17A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ18A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704) (partial sequence)
MJ19A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ20A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ22A. salmianaPhyllosphere (p)YesBacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae. (NR_109297)

ARA, Acetylene reduction assay as described in Hardy et al. (1968).

Cluster 1: strains grouped with OTUs 60, 61, and 62; Cluster 2: strains grouped with OTU 31; Cluster 3: strains grouped with OTU 38.

Table 4

Origin of Agave-associated bacterial strains grown in N-free culture media

Strain IDAgave speciesCommunity typeConfirmed ARA activitySILVA Taxonomic lineage (NCBI best hit accession number)DGGE cluster
MJ02A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ03A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ04A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_104933)1
MJ05A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ06A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ07A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104934)1
MJ08A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ09A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ10A. salmianaRhizosphere (rz)NoBacteria. (NR_116033) (partial sequence)
MJ11A. salmianaRhizosphere (rz)YesBacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus (NR_115953)2
MJ13A. salmianaRhizosphere (rz)NoBacteria. (NR_117704)
MJ14A. salmianaRhizosphere (rz)YesBacteria; Actinobacteria; Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium. (NR_044937)
MJ15A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704)
MJ17A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ18A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704) (partial sequence)
MJ19A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ20A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ22A. salmianaPhyllosphere (p)YesBacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae. (NR_109297)
Strain IDAgave speciesCommunity typeConfirmed ARA activitySILVA Taxonomic lineage (NCBI best hit accession number)DGGE cluster
MJ02A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ03A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_117704)
MJ04A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae. (NR_104933)1
MJ05A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ06A. tequilanaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ07A. tequilanaRoot endosphere (re)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104934)1
MJ08A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ09A. tequilanaRhizosphere (rz)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ10A. salmianaRhizosphere (rz)NoBacteria. (NR_116033) (partial sequence)
MJ11A. salmianaRhizosphere (rz)YesBacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus (NR_115953)2
MJ13A. salmianaRhizosphere (rz)NoBacteria. (NR_117704)
MJ14A. salmianaRhizosphere (rz)YesBacteria; Actinobacteria; Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium. (NR_044937)
MJ15A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704)
MJ17A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas. (NR_041577)3
MJ18A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_117704) (partial sequence)
MJ19A. salmianaRoot endosphere (re)YesBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_104933)1
MJ20A. salmianaRhizosphere (rz)NoBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;Enterobacteriaceae. (NR_044977)1
MJ22A. salmianaPhyllosphere (p)YesBacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae. (NR_109297)

ARA, Acetylene reduction assay as described in Hardy et al. (1968).

Cluster 1: strains grouped with OTUs 60, 61, and 62; Cluster 2: strains grouped with OTU 31; Cluster 3: strains grouped with OTU 38.

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