Table 1. Open in new tab The list of OMV...

Spot	Protein names^#2	Locus tags in	Locus tags in	Mascot	Peptide	Subcellular	Localization	Presence/length of	lipoprotein	Theoretical
1	receptor antigen A, RagA	PGN_0293	PG_0185	3404	237	OM	VM	Yes/20	NO	5.59 / 112196.92
2	minor componet FimD	PGN_0184	PG_2135	1082	30	U	ND	NO	NO	6.19 / 75848.09
3	Mfa1 fimbrillin	PGN_0287	ND	1291	92	U	ND	NO	Yes/19	5.62 / 60786.40
4	TonB-linked receptor Tlr	PGN_0683	PG_1552	1731	39	OM	VM	Yes/19	NO	7.31 / 76544.35
5	TonB-linked receptor Tlr	PGN_0683	PG_1552	1315	28	OM	VM	Yes/19	NO	7.31 / 76544.35
6	minor compent FimE	PGN_0185	PG_2136	1598	43	U	ND	Yes/24	Yes/21	6.17 / 58060.40
7	hypothetical protein PGN_0477	PGN_0477	PG_1626	1734	54	OM	VM	Yes/33	NO	6.07 / 57581.94
8	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1366	38	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
9	hypothetical protein PGN_0477	PGN_0477	PG_1626	1459	22	OM	VM	Yes/33	NO	6.07 / 57581.94
10	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	5419	126	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
11	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1418	116	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
12	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1326	45	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
13	receptor antigen B, RagB	PGN_0294	PG_0186	2224	137	U	VM(L)	Yes/24	Yes/19	5.30 / 54143.04
14	Por secretion system protein porN/gldN	PGN_1673	PG_0291	186	4	U	UL	Yes/22	NO	9.39 / 39290.31
15	Omp41 precursor	PGN_0729	PG_0695	272	6	OM	VM	Yes/20	NO	7.88 / 41300.66
16	Omp40 precursor	PGN_0728	PG_0694	1251	38	OM	VM	Yes/21	Yes/14	6.44 / 40370.50
17	FimA type I fimbrillin	PGN_0180	PG_2132	1250	164	EC	VM(L)	Yes/20	Yes/18	5.40 / 39211.97
18	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1551	89	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
19	hypothetical protein PGN_1557	PGN_1557	PG_0409	779	28	U	VM	Yes/21	NO	5.94 / 27289.94
20	heme-binding protein FetB	PGN_0705	PG_0669	795	16	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
21	heme-binding protein FetB	PGN_0705	PG_0669	1449	55	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
22	hypothetical protein PGN_0290	PGN_0290	PG_0181	1361	51	U	VM(L)	NO	NO	5.32 / 37110.19
23	hypothetical protein PGN_0860	PGN_0860	PG_1382	587	11	U	VM	Yes/21	Yes/19	8.54 / 36516.32
24	Omp41 precursor	PGN_0729	PG_0695	706	16	OM	VM	Yes/20	NO	7.88 / 41300.66
25	hypothetical protein PGN_0156	PGN_0156	PG_2106	891	29	U	VM	Yes/22	NO	9.12 / 22105.45
26	hypothetical protein PGN_1998	PGN_1998	PG_2054	674	24	OM	VM(L)	Yes/18	Yes/19	8.75 / 20828.41
27	hypothetical protein PGN_1744	PGN_1744	PG_1823	940	36	U	VM	Yes/43	NO	8.82 / 19941.71
28	Omp41 precursor	PGN_0729	PG_0695	650	15	OM	VM	Yes/20	NO	7.88 / 41300.66
29	hypothetical protein PGN_1744	PGN_1744	PG_1823	155	4	U	VM	Yes/43	NO	8.82 / 19941.71
30	Omp41 precursor	PGN_0729	PG_0695	816	29	OM	VM	Yes/20	NO	7.88 / 41300.66
31	zinc carboxypeptidase CPG70	PGN_0335	PG_0232	1515	76	U	EC-CTD	Yes/24	NO	5.07 / 88849.44
32	arginine-specific cysteine proteinase RgpB	PGN_1466	PG_0506	1372	72	U	EC-CTD	Yes/24	Yes/19	5.34 / 78372.87
33	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1163	36	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
34	TPR domain protein	PGN_0876	PG_1385	1304	31	U	VL	Yes/21	NO	5.17 / 43621.43
35	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1590	49	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
36	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	873	37	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
37	TPR domain protein	PGN_0876	PG_1385	1579	68	U	VL	Yes/21	NO	5.17 / 43621.43
38	Por secretion system protein porV (pg27, lptO)	PGN_0023	PG_0027	1377	58	OM	VM	Yes/26	NO	4.76 / 40312.12
39	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	870	28	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
40	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1144	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
41	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	635	15	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
42	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	902	38	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
43	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1032	40	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
44	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1163	54	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
45	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1173	60	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
46	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	608	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
47	hemagglutinin protein HagA	PGN_1733	PG_1837	594	27	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
48	hemagglutinin protein HagA	PGN_1733	PG_1837	438	40	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
49	immunoreactive 46 kDa antigen	PGN_1767	PG_1798	797	44	U	EC-CTD	U	NO	5.66 / 47696.75
50	hypothetical protein PGN_1816	PGN_1816	PG_1889	481	24	U	VM(L)	NO	Yes/20	5.65 / 24995.63
51	hypothetical protein PGN_1129	PGN_1129	PG_1341	709	49	U	VM(L)	Yes/18	Yes/19	6.38 / 15644.45
52	hemagglutinin protein HagA	PGN_1733	PG_1837	561	21	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
53	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	607	20	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40

Spot	Protein names^#2	Locus tags in	Locus tags in	Mascot	Peptide	Subcellular	Localization	Presence/length of	lipoprotein	Theoretical
1	receptor antigen A, RagA	PGN_0293	PG_0185	3404	237	OM	VM	Yes/20	NO	5.59 / 112196.92
2	minor componet FimD	PGN_0184	PG_2135	1082	30	U	ND	NO	NO	6.19 / 75848.09
3	Mfa1 fimbrillin	PGN_0287	ND	1291	92	U	ND	NO	Yes/19	5.62 / 60786.40
4	TonB-linked receptor Tlr	PGN_0683	PG_1552	1731	39	OM	VM	Yes/19	NO	7.31 / 76544.35
5	TonB-linked receptor Tlr	PGN_0683	PG_1552	1315	28	OM	VM	Yes/19	NO	7.31 / 76544.35
6	minor compent FimE	PGN_0185	PG_2136	1598	43	U	ND	Yes/24	Yes/21	6.17 / 58060.40
7	hypothetical protein PGN_0477	PGN_0477	PG_1626	1734	54	OM	VM	Yes/33	NO	6.07 / 57581.94
8	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1366	38	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
9	hypothetical protein PGN_0477	PGN_0477	PG_1626	1459	22	OM	VM	Yes/33	NO	6.07 / 57581.94
10	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	5419	126	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
11	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1418	116	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
12	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1326	45	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
13	receptor antigen B, RagB	PGN_0294	PG_0186	2224	137	U	VM(L)	Yes/24	Yes/19	5.30 / 54143.04
14	Por secretion system protein porN/gldN	PGN_1673	PG_0291	186	4	U	UL	Yes/22	NO	9.39 / 39290.31
15	Omp41 precursor	PGN_0729	PG_0695	272	6	OM	VM	Yes/20	NO	7.88 / 41300.66
16	Omp40 precursor	PGN_0728	PG_0694	1251	38	OM	VM	Yes/21	Yes/14	6.44 / 40370.50
17	FimA type I fimbrillin	PGN_0180	PG_2132	1250	164	EC	VM(L)	Yes/20	Yes/18	5.40 / 39211.97
18	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1551	89	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
19	hypothetical protein PGN_1557	PGN_1557	PG_0409	779	28	U	VM	Yes/21	NO	5.94 / 27289.94
20	heme-binding protein FetB	PGN_0705	PG_0669	795	16	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
21	heme-binding protein FetB	PGN_0705	PG_0669	1449	55	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
22	hypothetical protein PGN_0290	PGN_0290	PG_0181	1361	51	U	VM(L)	NO	NO	5.32 / 37110.19
23	hypothetical protein PGN_0860	PGN_0860	PG_1382	587	11	U	VM	Yes/21	Yes/19	8.54 / 36516.32
24	Omp41 precursor	PGN_0729	PG_0695	706	16	OM	VM	Yes/20	NO	7.88 / 41300.66
25	hypothetical protein PGN_0156	PGN_0156	PG_2106	891	29	U	VM	Yes/22	NO	9.12 / 22105.45
26	hypothetical protein PGN_1998	PGN_1998	PG_2054	674	24	OM	VM(L)	Yes/18	Yes/19	8.75 / 20828.41
27	hypothetical protein PGN_1744	PGN_1744	PG_1823	940	36	U	VM	Yes/43	NO	8.82 / 19941.71
28	Omp41 precursor	PGN_0729	PG_0695	650	15	OM	VM	Yes/20	NO	7.88 / 41300.66
29	hypothetical protein PGN_1744	PGN_1744	PG_1823	155	4	U	VM	Yes/43	NO	8.82 / 19941.71
30	Omp41 precursor	PGN_0729	PG_0695	816	29	OM	VM	Yes/20	NO	7.88 / 41300.66
31	zinc carboxypeptidase CPG70	PGN_0335	PG_0232	1515	76	U	EC-CTD	Yes/24	NO	5.07 / 88849.44
32	arginine-specific cysteine proteinase RgpB	PGN_1466	PG_0506	1372	72	U	EC-CTD	Yes/24	Yes/19	5.34 / 78372.87
33	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1163	36	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
34	TPR domain protein	PGN_0876	PG_1385	1304	31	U	VL	Yes/21	NO	5.17 / 43621.43
35	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1590	49	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
36	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	873	37	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
37	TPR domain protein	PGN_0876	PG_1385	1579	68	U	VL	Yes/21	NO	5.17 / 43621.43
38	Por secretion system protein porV (pg27, lptO)	PGN_0023	PG_0027	1377	58	OM	VM	Yes/26	NO	4.76 / 40312.12
39	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	870	28	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
40	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1144	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
41	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	635	15	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
42	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	902	38	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
43	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1032	40	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
44	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1163	54	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
45	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1173	60	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
46	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	608	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
47	hemagglutinin protein HagA	PGN_1733	PG_1837	594	27	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
48	hemagglutinin protein HagA	PGN_1733	PG_1837	438	40	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
49	immunoreactive 46 kDa antigen	PGN_1767	PG_1798	797	44	U	EC-CTD	U	NO	5.66 / 47696.75
50	hypothetical protein PGN_1816	PGN_1816	PG_1889	481	24	U	VM(L)	NO	Yes/20	5.65 / 24995.63
51	hypothetical protein PGN_1129	PGN_1129	PG_1341	709	49	U	VM(L)	Yes/18	Yes/19	6.38 / 15644.45
52	hemagglutinin protein HagA	PGN_1733	PG_1837	561	21	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
53	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	607	20	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40

Table 1.

The list of OMV proteins appeared on a 2D-PAGE gel.

Spot	Protein names^#2	Locus tags in	Locus tags in	Mascot	Peptide	Subcellular	Localization	Presence/length of	lipoprotein	Theoretical
nos.^#1		ATCC 33277	W83	scores	numbers	localization^#5	in OMVs^#6	signal peptide^#7	signal	pI/Mw^#9
		database^#3	database^#4						peptide^#8
1	receptor antigen A, RagA	PGN_0293	PG_0185	3404	237	OM	VM	Yes/20	NO	5.59 / 112196.92
2	minor componet FimD	PGN_0184	PG_2135	1082	30	U	ND	NO	NO	6.19 / 75848.09
3	Mfa1 fimbrillin	PGN_0287	ND	1291	92	U	ND	NO	Yes/19	5.62 / 60786.40
4	TonB-linked receptor Tlr	PGN_0683	PG_1552	1731	39	OM	VM	Yes/19	NO	7.31 / 76544.35
5	TonB-linked receptor Tlr	PGN_0683	PG_1552	1315	28	OM	VM	Yes/19	NO	7.31 / 76544.35
6	minor compent FimE	PGN_0185	PG_2136	1598	43	U	ND	Yes/24	Yes/21	6.17 / 58060.40
7	hypothetical protein PGN_0477	PGN_0477	PG_1626	1734	54	OM	VM	Yes/33	NO	6.07 / 57581.94
8	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1366	38	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
9	hypothetical protein PGN_0477	PGN_0477	PG_1626	1459	22	OM	VM	Yes/33	NO	6.07 / 57581.94
10	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	5419	126	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
11	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1418	116	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
12	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1326	45	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
13	receptor antigen B, RagB	PGN_0294	PG_0186	2224	137	U	VM(L)	Yes/24	Yes/19	5.30 / 54143.04
14	Por secretion system protein porN/gldN	PGN_1673	PG_0291	186	4	U	UL	Yes/22	NO	9.39 / 39290.31
15	Omp41 precursor	PGN_0729	PG_0695	272	6	OM	VM	Yes/20	NO	7.88 / 41300.66
16	Omp40 precursor	PGN_0728	PG_0694	1251	38	OM	VM	Yes/21	Yes/14	6.44 / 40370.50
17	FimA type I fimbrillin	PGN_0180	PG_2132	1250	164	EC	VM(L)	Yes/20	Yes/18	5.40 / 39211.97
18	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1551	89	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
19	hypothetical protein PGN_1557	PGN_1557	PG_0409	779	28	U	VM	Yes/21	NO	5.94 / 27289.94
20	heme-binding protein FetB	PGN_0705	PG_0669	795	16	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
21	heme-binding protein FetB	PGN_0705	PG_0669	1449	55	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
22	hypothetical protein PGN_0290	PGN_0290	PG_0181	1361	51	U	VM(L)	NO	NO	5.32 / 37110.19
23	hypothetical protein PGN_0860	PGN_0860	PG_1382	587	11	U	VM	Yes/21	Yes/19	8.54 / 36516.32
24	Omp41 precursor	PGN_0729	PG_0695	706	16	OM	VM	Yes/20	NO	7.88 / 41300.66
25	hypothetical protein PGN_0156	PGN_0156	PG_2106	891	29	U	VM	Yes/22	NO	9.12 / 22105.45
26	hypothetical protein PGN_1998	PGN_1998	PG_2054	674	24	OM	VM(L)	Yes/18	Yes/19	8.75 / 20828.41
27	hypothetical protein PGN_1744	PGN_1744	PG_1823	940	36	U	VM	Yes/43	NO	8.82 / 19941.71
28	Omp41 precursor	PGN_0729	PG_0695	650	15	OM	VM	Yes/20	NO	7.88 / 41300.66
29	hypothetical protein PGN_1744	PGN_1744	PG_1823	155	4	U	VM	Yes/43	NO	8.82 / 19941.71
30	Omp41 precursor	PGN_0729	PG_0695	816	29	OM	VM	Yes/20	NO	7.88 / 41300.66
31	zinc carboxypeptidase CPG70	PGN_0335	PG_0232	1515	76	U	EC-CTD	Yes/24	NO	5.07 / 88849.44
32	arginine-specific cysteine proteinase RgpB	PGN_1466	PG_0506	1372	72	U	EC-CTD	Yes/24	Yes/19	5.34 / 78372.87
33	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1163	36	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
34	TPR domain protein	PGN_0876	PG_1385	1304	31	U	VL	Yes/21	NO	5.17 / 43621.43
35	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1590	49	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
36	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	873	37	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
37	TPR domain protein	PGN_0876	PG_1385	1579	68	U	VL	Yes/21	NO	5.17 / 43621.43
38	Por secretion system protein porV (pg27, lptO)	PGN_0023	PG_0027	1377	58	OM	VM	Yes/26	NO	4.76 / 40312.12
39	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	870	28	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
40	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1144	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
41	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	635	15	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
42	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	902	38	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
43	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1032	40	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
44	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1163	54	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
45	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1173	60	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
46	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	608	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
47	hemagglutinin protein HagA	PGN_1733	PG_1837	594	27	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
48	hemagglutinin protein HagA	PGN_1733	PG_1837	438	40	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
49	immunoreactive 46 kDa antigen	PGN_1767	PG_1798	797	44	U	EC-CTD	U	NO	5.66 / 47696.75
50	hypothetical protein PGN_1816	PGN_1816	PG_1889	481	24	U	VM(L)	NO	Yes/20	5.65 / 24995.63
51	hypothetical protein PGN_1129	PGN_1129	PG_1341	709	49	U	VM(L)	Yes/18	Yes/19	6.38 / 15644.45
52	hemagglutinin protein HagA	PGN_1733	PG_1837	561	21	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
53	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	607	20	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40

Spot	Protein names^#2	Locus tags in	Locus tags in	Mascot	Peptide	Subcellular	Localization	Presence/length of	lipoprotein	Theoretical
nos.^#1		ATCC 33277	W83	scores	numbers	localization^#5	in OMVs^#6	signal peptide^#7	signal	pI/Mw^#9
		database^#3	database^#4						peptide^#8
1	receptor antigen A, RagA	PGN_0293	PG_0185	3404	237	OM	VM	Yes/20	NO	5.59 / 112196.92
2	minor componet FimD	PGN_0184	PG_2135	1082	30	U	ND	NO	NO	6.19 / 75848.09
3	Mfa1 fimbrillin	PGN_0287	ND	1291	92	U	ND	NO	Yes/19	5.62 / 60786.40
4	TonB-linked receptor Tlr	PGN_0683	PG_1552	1731	39	OM	VM	Yes/19	NO	7.31 / 76544.35
5	TonB-linked receptor Tlr	PGN_0683	PG_1552	1315	28	OM	VM	Yes/19	NO	7.31 / 76544.35
6	minor compent FimE	PGN_0185	PG_2136	1598	43	U	ND	Yes/24	Yes/21	6.17 / 58060.40
7	hypothetical protein PGN_0477	PGN_0477	PG_1626	1734	54	OM	VM	Yes/33	NO	6.07 / 57581.94
8	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1366	38	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
9	hypothetical protein PGN_0477	PGN_0477	PG_1626	1459	22	OM	VM	Yes/33	NO	6.07 / 57581.94
10	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	5419	126	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
11	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1418	116	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
12	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1326	45	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
13	receptor antigen B, RagB	PGN_0294	PG_0186	2224	137	U	VM(L)	Yes/24	Yes/19	5.30 / 54143.04
14	Por secretion system protein porN/gldN	PGN_1673	PG_0291	186	4	U	UL	Yes/22	NO	9.39 / 39290.31
15	Omp41 precursor	PGN_0729	PG_0695	272	6	OM	VM	Yes/20	NO	7.88 / 41300.66
16	Omp40 precursor	PGN_0728	PG_0694	1251	38	OM	VM	Yes/21	Yes/14	6.44 / 40370.50
17	FimA type I fimbrillin	PGN_0180	PG_2132	1250	164	EC	VM(L)	Yes/20	Yes/18	5.40 / 39211.97
18	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1551	89	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
19	hypothetical protein PGN_1557	PGN_1557	PG_0409	779	28	U	VM	Yes/21	NO	5.94 / 27289.94
20	heme-binding protein FetB	PGN_0705	PG_0669	795	16	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
21	heme-binding protein FetB	PGN_0705	PG_0669	1449	55	U	VM(L)	Yes/23	Yes/18	5.58 / 30189.26
22	hypothetical protein PGN_0290	PGN_0290	PG_0181	1361	51	U	VM(L)	NO	NO	5.32 / 37110.19
23	hypothetical protein PGN_0860	PGN_0860	PG_1382	587	11	U	VM	Yes/21	Yes/19	8.54 / 36516.32
24	Omp41 precursor	PGN_0729	PG_0695	706	16	OM	VM	Yes/20	NO	7.88 / 41300.66
25	hypothetical protein PGN_0156	PGN_0156	PG_2106	891	29	U	VM	Yes/22	NO	9.12 / 22105.45
26	hypothetical protein PGN_1998	PGN_1998	PG_2054	674	24	OM	VM(L)	Yes/18	Yes/19	8.75 / 20828.41
27	hypothetical protein PGN_1744	PGN_1744	PG_1823	940	36	U	VM	Yes/43	NO	8.82 / 19941.71
28	Omp41 precursor	PGN_0729	PG_0695	650	15	OM	VM	Yes/20	NO	7.88 / 41300.66
29	hypothetical protein PGN_1744	PGN_1744	PG_1823	155	4	U	VM	Yes/43	NO	8.82 / 19941.71
30	Omp41 precursor	PGN_0729	PG_0695	816	29	OM	VM	Yes/20	NO	7.88 / 41300.66
31	zinc carboxypeptidase CPG70	PGN_0335	PG_0232	1515	76	U	EC-CTD	Yes/24	NO	5.07 / 88849.44
32	arginine-specific cysteine proteinase RgpB	PGN_1466	PG_0506	1372	72	U	EC-CTD	Yes/24	Yes/19	5.34 / 78372.87
33	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1163	36	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
34	TPR domain protein	PGN_0876	PG_1385	1304	31	U	VL	Yes/21	NO	5.17 / 43621.43
35	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	1590	49	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
36	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	873	37	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
37	TPR domain protein	PGN_0876	PG_1385	1579	68	U	VL	Yes/21	NO	5.17 / 43621.43
38	Por secretion system protein porV (pg27, lptO)	PGN_0023	PG_0027	1377	58	OM	VM	Yes/26	NO	4.76 / 40312.12
39	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	870	28	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
40	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1144	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
41	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	635	15	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40
42	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	902	38	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
43	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1032	40	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
44	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1163	54	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
45	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	1173	60	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
46	arginine-specific cysteine proteinase RgpA	PGN_1970	PG_2024	608	35	EC	EC-CTD	Yes/20	NO	4.96 / 183282.09
47	hemagglutinin protein HagA	PGN_1733	PG_1837	594	27	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
48	hemagglutinin protein HagA	PGN_1733	PG_1837	438	40	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
49	immunoreactive 46 kDa antigen	PGN_1767	PG_1798	797	44	U	EC-CTD	U	NO	5.66 / 47696.75
50	hypothetical protein PGN_1816	PGN_1816	PG_1889	481	24	U	VM(L)	NO	Yes/20	5.65 / 24995.63
51	hypothetical protein PGN_1129	PGN_1129	PG_1341	709	49	U	VM(L)	Yes/18	Yes/19	6.38 / 15644.45
52	hemagglutinin protein HagA	PGN_1733	PG_1837	561	21	U	EC-CTD	Yes/24	Yes/17	4.60 / 280580.19
53	lysine-specific cysteine proteinase Kgp	PGN_1728	PG_1844	607	20	EC	EC-CTD	Yes/21	NO	4.95 / 185049.40

#1: The corresponding spot number on the reference on 2D-PAGE protein map in Fig. 3. The proteins with highest mascot score were shown in the list.

#2: The names of proteins in bold represent CTD proteins.

#3: The accession number in the genome sequence of P. gingivalis ATCC 33277.

#4: The accession number in the genome sequence of P. gingivalis W83.ND; not detected.

#5: Cellular localization predicted by PSORTb program. EC; extracellular, OM; outer membrane, PP; periplasm, IM; inner membrane, CP; cytoplasm, U; unknown.

#6: Localization in OMVs which was predicted and reported by Veith P.D. et al. in 2014. VM; vesicle membrane, VM(L); vesicle membrane (lipoproteins), VL; vesicle lumen, EC-CTD; Extracellular CTD-containing proteins, UL; uncertain location, ND; not detected.

#7: Presence/length of signal peptides predicted by SignalP program.

#8: Lipoprotein signal peptides predicted by LipoP program.

#9: Theoretical pI/molecular weight predicted by ExPaSy.

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