Table 3.
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Frequency of 3′ UTR reporter repression for artificial miRNAs with canonical, bulge or no seed matches
Artificial miRNA seed match in reportermiRNAs with matches (total for all reporters)Reporter repressedReporter not repressed% Reporter repressedP values, Fisher's test
Canonical versus noneCanonical versus bulgeBulge versus none
None362323308.8%< 10−130.100.00031
Canonical2267615033.6%
Bulge88216723.9%
Artificial miRNA seed match in reportermiRNAs with matches (total for all reporters)Reporter repressedReporter not repressed% Reporter repressedP values, Fisher's test
Canonical versus noneCanonical versus bulgeBulge versus none
None362323308.8%< 10−130.100.00031
Canonical2267615033.6%
Bulge88216723.9%

Each pair of artificial miRNA and 3′ UTR reporter (PC, GLS or GAPDH) was classified by whether that miRNA had a canonical, bulge or no seed match in the 3′ UTR of the reporter. For each seed match category, the miRNA / reporter pairs were further classified by whether the miRNA significantly repressed the reporter luciferase activity. The significance of the association between seed match category and reporter repression was calculated using Fisher's exact test for all pairs of seed match categories.

Table 3.
Open in new tab
Frequency of 3′ UTR reporter repression for artificial miRNAs with canonical, bulge or no seed matches
Artificial miRNA seed match in reportermiRNAs with matches (total for all reporters)Reporter repressedReporter not repressed% Reporter repressedP values, Fisher's test
Canonical versus noneCanonical versus bulgeBulge versus none
None362323308.8%< 10−130.100.00031
Canonical2267615033.6%
Bulge88216723.9%
Artificial miRNA seed match in reportermiRNAs with matches (total for all reporters)Reporter repressedReporter not repressed% Reporter repressedP values, Fisher's test
Canonical versus noneCanonical versus bulgeBulge versus none
None362323308.8%< 10−130.100.00031
Canonical2267615033.6%
Bulge88216723.9%

Each pair of artificial miRNA and 3′ UTR reporter (PC, GLS or GAPDH) was classified by whether that miRNA had a canonical, bulge or no seed match in the 3′ UTR of the reporter. For each seed match category, the miRNA / reporter pairs were further classified by whether the miRNA significantly repressed the reporter luciferase activity. The significance of the association between seed match category and reporter repression was calculated using Fisher's exact test for all pairs of seed match categories.

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