Table 1.
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Inhibition spectra of PR66 against enteroviruses or influenza viruses

Cell line or virus strainPR66
CC50 (μM)aIC50 (μM)bSIc
Cytotoxic effectd
 A549>100
 MDCK3.466 ± 2.089
 RD>100
Enterovirus
 EV71 BrCr (genotype A)0.028 ± 0.009+++
 EV71 TW/2231/98 (genotype C)0.019 ± 0.001+++
 EV71 TW/4643/98 (genotype C)0.026 ± 0.005+++
 EV71 TW/51126/12 (genotype B)0.024 ± 0.010+++
 EV71 TW/2557/12 (genotype B)0.028 ± 0.010+++
 EV71 TW/50995/12 (genotype B)0.058 ± 0.002+++
 EV71 TW/51045/12 (genotype B)0.032 ± 0.001+++
 EV71 TW/184/12 (genotype C)0.008 ± 0.003++++
 EV71 TW/73/12 (genotype C)0.006 ± 0.003++++
 EV-D68>10−
 coxsackievirus A160.188 ± 0.062++
 coxsackievirus B1>25−
 coxsackievirus B2>25−
 coxsackievirus B3>25−
 echovirus 9>25−
 human rhinovirus sp.>25
Influenza virus
 A/WSN/33 (H1N1)>5−
 B/TW/70325/05>5−
Herpes simplex virus-1>10−
Adenovirus>10−
Cell line or virus strainPR66
CC50 (μM)aIC50 (μM)bSIc
Cytotoxic effectd
 A549>100
 MDCK3.466 ± 2.089
 RD>100
Enterovirus
 EV71 BrCr (genotype A)0.028 ± 0.009+++
 EV71 TW/2231/98 (genotype C)0.019 ± 0.001+++
 EV71 TW/4643/98 (genotype C)0.026 ± 0.005+++
 EV71 TW/51126/12 (genotype B)0.024 ± 0.010+++
 EV71 TW/2557/12 (genotype B)0.028 ± 0.010+++
 EV71 TW/50995/12 (genotype B)0.058 ± 0.002+++
 EV71 TW/51045/12 (genotype B)0.032 ± 0.001+++
 EV71 TW/184/12 (genotype C)0.008 ± 0.003++++
 EV71 TW/73/12 (genotype C)0.006 ± 0.003++++
 EV-D68>10−
 coxsackievirus A160.188 ± 0.062++
 coxsackievirus B1>25−
 coxsackievirus B2>25−
 coxsackievirus B3>25−
 echovirus 9>25−
 human rhinovirus sp.>25
Influenza virus
 A/WSN/33 (H1N1)>5−
 B/TW/70325/05>5−
Herpes simplex virus-1>10−
Adenovirus>10−

Values are the mean ± SD of the results from two to three independent experiments.

aCC50 was determined with an MTT assay.

bIC50 was determined with an anti-CPE (neutralization) assay.

cSelectivity index (SI) was defined as the ratio of CC50 to IC50 values: ++, SI >100; +++, SI >1000; ++++, SI >10 000; and −, not available for determination.

dCytotoxicities of enteroviruses and human rhinovirus were determined in RD cells, whereas those of influenza viruses were studied in MDCK cells and those of herpes simplex virus-1 and adenovirus were studied in A549 cells.

Table 1.
Open in new tab

Inhibition spectra of PR66 against enteroviruses or influenza viruses

Cell line or virus strainPR66
CC50 (μM)aIC50 (μM)bSIc
Cytotoxic effectd
 A549>100
 MDCK3.466 ± 2.089
 RD>100
Enterovirus
 EV71 BrCr (genotype A)0.028 ± 0.009+++
 EV71 TW/2231/98 (genotype C)0.019 ± 0.001+++
 EV71 TW/4643/98 (genotype C)0.026 ± 0.005+++
 EV71 TW/51126/12 (genotype B)0.024 ± 0.010+++
 EV71 TW/2557/12 (genotype B)0.028 ± 0.010+++
 EV71 TW/50995/12 (genotype B)0.058 ± 0.002+++
 EV71 TW/51045/12 (genotype B)0.032 ± 0.001+++
 EV71 TW/184/12 (genotype C)0.008 ± 0.003++++
 EV71 TW/73/12 (genotype C)0.006 ± 0.003++++
 EV-D68>10−
 coxsackievirus A160.188 ± 0.062++
 coxsackievirus B1>25−
 coxsackievirus B2>25−
 coxsackievirus B3>25−
 echovirus 9>25−
 human rhinovirus sp.>25
Influenza virus
 A/WSN/33 (H1N1)>5−
 B/TW/70325/05>5−
Herpes simplex virus-1>10−
Adenovirus>10−
Cell line or virus strainPR66
CC50 (μM)aIC50 (μM)bSIc
Cytotoxic effectd
 A549>100
 MDCK3.466 ± 2.089
 RD>100
Enterovirus
 EV71 BrCr (genotype A)0.028 ± 0.009+++
 EV71 TW/2231/98 (genotype C)0.019 ± 0.001+++
 EV71 TW/4643/98 (genotype C)0.026 ± 0.005+++
 EV71 TW/51126/12 (genotype B)0.024 ± 0.010+++
 EV71 TW/2557/12 (genotype B)0.028 ± 0.010+++
 EV71 TW/50995/12 (genotype B)0.058 ± 0.002+++
 EV71 TW/51045/12 (genotype B)0.032 ± 0.001+++
 EV71 TW/184/12 (genotype C)0.008 ± 0.003++++
 EV71 TW/73/12 (genotype C)0.006 ± 0.003++++
 EV-D68>10−
 coxsackievirus A160.188 ± 0.062++
 coxsackievirus B1>25−
 coxsackievirus B2>25−
 coxsackievirus B3>25−
 echovirus 9>25−
 human rhinovirus sp.>25
Influenza virus
 A/WSN/33 (H1N1)>5−
 B/TW/70325/05>5−
Herpes simplex virus-1>10−
Adenovirus>10−

Values are the mean ± SD of the results from two to three independent experiments.

aCC50 was determined with an MTT assay.

bIC50 was determined with an anti-CPE (neutralization) assay.

cSelectivity index (SI) was defined as the ratio of CC50 to IC50 values: ++, SI >100; +++, SI >1000; ++++, SI >10 000; and −, not available for determination.

dCytotoxicities of enteroviruses and human rhinovirus were determined in RD cells, whereas those of influenza viruses were studied in MDCK cells and those of herpes simplex virus-1 and adenovirus were studied in A549 cells.

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