Table 1.

Antibodies Used

Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog No., and/or Name of Individual Providing AntibodySpecies Raised in; Monoclonal or PolyclonalDilution UsedRRID
Histone H3 Lys27Human histone H3aa 1-100 (acetyl K27)Anti-acetyl histone H3 Lys27 (H3K27ac)Abcam, Ab4729Rabbit; polyclonal1:200AB_2118291
β-ActinN-terminus of human β-actinβ-Actin (ACTB)Cell Signaling Technology, catalog no. 4967Rabbit; polyclonal1:2000AB_330288
Rabbit IgGRabbit IgG, whole antibodyHorseradish peroxidase-linked anti-rabbit IgGAmersham Biosciences, catalog no. 934Donkey1:3000AB_772211
TRβMNYCMPEVHE VCPAASSNCY MQVTDYLAYL EDSPALSGRD VQAVPSSSIYAnti-thyroid hormone receptor-βAbcam, Ab104417Rabbit; polyclonal1:250AB_10716599
Histone H3 Lys27ARacKSA, in which acK corresponds to acetyl lysine27 of yest histone H3Anti-acetyl histone H3 Lys27 (H3K27ac)Millipore, catalog no. 07-360Rabbit; polyclonal1:250AB_310550
No targetNormal rabbit serum (IgG)Cell Signaling Technology, catalog no. 2729Rabbit1:500AB_2617119
Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog No., and/or Name of Individual Providing AntibodySpecies Raised in; Monoclonal or PolyclonalDilution UsedRRID
Histone H3 Lys27Human histone H3aa 1-100 (acetyl K27)Anti-acetyl histone H3 Lys27 (H3K27ac)Abcam, Ab4729Rabbit; polyclonal1:200AB_2118291
β-ActinN-terminus of human β-actinβ-Actin (ACTB)Cell Signaling Technology, catalog no. 4967Rabbit; polyclonal1:2000AB_330288
Rabbit IgGRabbit IgG, whole antibodyHorseradish peroxidase-linked anti-rabbit IgGAmersham Biosciences, catalog no. 934Donkey1:3000AB_772211
TRβMNYCMPEVHE VCPAASSNCY MQVTDYLAYL EDSPALSGRD VQAVPSSSIYAnti-thyroid hormone receptor-βAbcam, Ab104417Rabbit; polyclonal1:250AB_10716599
Histone H3 Lys27ARacKSA, in which acK corresponds to acetyl lysine27 of yest histone H3Anti-acetyl histone H3 Lys27 (H3K27ac)Millipore, catalog no. 07-360Rabbit; polyclonal1:250AB_310550
No targetNormal rabbit serum (IgG)Cell Signaling Technology, catalog no. 2729Rabbit1:500AB_2617119

Abbreviation: RRID, Resource Identification.

Table 1.

Antibodies Used

Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog No., and/or Name of Individual Providing AntibodySpecies Raised in; Monoclonal or PolyclonalDilution UsedRRID
Histone H3 Lys27Human histone H3aa 1-100 (acetyl K27)Anti-acetyl histone H3 Lys27 (H3K27ac)Abcam, Ab4729Rabbit; polyclonal1:200AB_2118291
β-ActinN-terminus of human β-actinβ-Actin (ACTB)Cell Signaling Technology, catalog no. 4967Rabbit; polyclonal1:2000AB_330288
Rabbit IgGRabbit IgG, whole antibodyHorseradish peroxidase-linked anti-rabbit IgGAmersham Biosciences, catalog no. 934Donkey1:3000AB_772211
TRβMNYCMPEVHE VCPAASSNCY MQVTDYLAYL EDSPALSGRD VQAVPSSSIYAnti-thyroid hormone receptor-βAbcam, Ab104417Rabbit; polyclonal1:250AB_10716599
Histone H3 Lys27ARacKSA, in which acK corresponds to acetyl lysine27 of yest histone H3Anti-acetyl histone H3 Lys27 (H3K27ac)Millipore, catalog no. 07-360Rabbit; polyclonal1:250AB_310550
No targetNormal rabbit serum (IgG)Cell Signaling Technology, catalog no. 2729Rabbit1:500AB_2617119
Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog No., and/or Name of Individual Providing AntibodySpecies Raised in; Monoclonal or PolyclonalDilution UsedRRID
Histone H3 Lys27Human histone H3aa 1-100 (acetyl K27)Anti-acetyl histone H3 Lys27 (H3K27ac)Abcam, Ab4729Rabbit; polyclonal1:200AB_2118291
β-ActinN-terminus of human β-actinβ-Actin (ACTB)Cell Signaling Technology, catalog no. 4967Rabbit; polyclonal1:2000AB_330288
Rabbit IgGRabbit IgG, whole antibodyHorseradish peroxidase-linked anti-rabbit IgGAmersham Biosciences, catalog no. 934Donkey1:3000AB_772211
TRβMNYCMPEVHE VCPAASSNCY MQVTDYLAYL EDSPALSGRD VQAVPSSSIYAnti-thyroid hormone receptor-βAbcam, Ab104417Rabbit; polyclonal1:250AB_10716599
Histone H3 Lys27ARacKSA, in which acK corresponds to acetyl lysine27 of yest histone H3Anti-acetyl histone H3 Lys27 (H3K27ac)Millipore, catalog no. 07-360Rabbit; polyclonal1:250AB_310550
No targetNormal rabbit serum (IgG)Cell Signaling Technology, catalog no. 2729Rabbit1:500AB_2617119

Abbreviation: RRID, Resource Identification.

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